Cell Structure and Function

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Cell Structure and Function

• This is a transmission electron micrograph of a
  neutrophil, a cell found in bone marrow
• Color has been added to highlight the various
  organelles (magnification 27,500)

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Life Is Cellular

• Look closely at a part of a living thing, and
  what do you see?
• Hold a blade of grass up against the light, and
  you see tiny lines running the length of the
  blade
• Examine the tip of your finger, and you see the
  ridges and valleys that make up fingerprints
• Place an insect under a microscope, and you see
  the intricate structures of its wings and the
  spikes and bristles that protect its body
• As interesting as these close-up views may be,
  however, they're only the beginning of the story
• Look closer and deeper with a more powerful
  microscope, and you'll see that there is a common
  structure that makes up every living thing the
  cell

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The Discovery of the Cell

• Seeing is believing, an old saying goes
• It would be hard to find a better example of this
  than the discovery of the cell
• Without the instruments to make them visible,
  cells remained out of sight and, therefore, out
  of mind for most of human history
• All of this changed with a dramatic advance in
  technology the invention of the microscope

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Early Microscopes 

• It was not until the mid-1600s that scientists
  began to use microscopes to observe living things
• In 1665, Englishman Robert Hooke used an early
  compound microscope to look at a thin slice of
  cork, a plant material
• Under the microscope, cork seemed to be made of
  thousands of tiny, empty chambers
• Hooke called these chambers cells because they
  reminded him of a monastery's tiny rooms, which
  were called cells
• One of Hooke's illustrations of cells is shown in
  the figure to the right
• The term cell is used in biology to this day
• We now know, however, that cells are not empty
  but contain living matter

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Cork Cells   

• Using an early microscope, Hooke made this
  drawing of cork cells
• In Hooke's drawings, the cells look like empty
  chambers because he was looking at dead plant
  matter
• Today, we know that living cells are made up of
  many structures

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Early Microscopes

• In Holland around the same time, Anton van
  Leeuwenhoek used a single-lens microscope to
  observe pond water and other things
• To his amazement, the microscope revealed a
  fantastic world of tiny living organisms that
  seemed to be everywhere, even in the very water
  he and his neighbors drank

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The Cell Theory 

• Soon, numerous observations made it clear that
  cells were the basic units of life
• In 1838, German botanist Matthias Schleiden
  concluded that all plants were made of cells
• The next year, German biologist Theodor Schwann
  stated that all animals were made of cells
• In 1855, the German physician Rudolf Virchow
  concluded that new cells could be produced only
  from the division of existing cells
• These discoveries, confirmed by other biologists,
  are summarized in the cell theory, a fundamental
  concept of biology

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The Cell Theory

• The cell theory states
• All living things are composed of cells
• Cells are the basic units of structure and
  function in living things
• New cells are produced from existing cells

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Exploring the Cell

• Following in the footsteps of Hooke, Virchow, and
  others, modern biologists still use microscopes
  to explore the cell
• However, today's researchers use microscopes and
  techniques more powerful than the pioneers of
  biology could have imagined
• Researchers can use fluorescent labels and light
  microscopy to follow molecules moving through the
  cell
• Confocal light microscopy, which scans cells with
  a laser beam, makes it possible to build
  three-dimensional images of cells and their parts
• High-resolution video technology makes it easy to
  produce movies of cells as they grow, divide, and
  develop

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Exploring the Cell

• These new technologies make it possible for
  researchers to study the structure and movement
  of living cells in great detail
• Unfortunately, light itself limits the detail, or
  resolution, of images that can be made with the
  light microscope
• Like all forms of radiation, light waves are
  diffracted, or scattered, as they pass through
  matter, making it impossible to visualize tiny
  structures such as proteins and viruses with
  light microscopy

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Exploring the Cell

• By contrast, as shown in the figure below,
  electron microscopes are capable of revealing
  details as much as 1000 times smaller than those
  visible in light microscopes because the
  wavelengths of electrons are much shorter than
  those of light
• Transmission electron microscopes (TEMs) make it
  possible to explore cell structures and large
  protein molecules
• Because beams of electrons can only pass through
  thin samples, cells and tissues must be cut first
  into ultrathin slices before they can be examined
  under a microscope

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Exploring the Cell
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Exploring the Cell

• With scanning electron microscopes (SEMs), a
  pencillike beam of electrons is scanned over the
  surface of a specimen
• For SEM images, specimens do not have to be cut
  into thin slices to be visualized
• The scanning electron microscope produces
  stunning three-dimensional images of cells
• Because electrons are easily scattered by
  molecules in the air, samples examined in both
  types of electron microscopes must be placed in a
  vacuum in order to be studied
• As a result, researchers chemically preserve
  their samples first and then carefully remove all
  of the water before placing them in the
  microscope
• This means that electron microscopy can be used
  to visualize only nonliving, preserved cells and
  tissues

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Exploring the Cell

• In the 1990s, researchers perfected a new class
  of microscopes that produce images by tracing the
  surfaces of samples with a fine probe
• These scanning probe microscopes have
  revolutionized the study of surfaces and made it
  possible to observe single atoms
• Unlike electron microscopes, scanning probe
  microscopes can operate in ordinary air and can
  even show samples in solution
• Researchers have already used scanning probe
  microscopes to image DNA and protein molecules as
  well as a number of important biological
  structures

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Prokaryotes and Eukaryotes

• Cells come in a great variety of shapes and an
  amazing range of sizes
• Although typical cells range from 5 to 50
  micrometers in diameter, the tiniest mycoplasma
  bacteria are only 0.2 micrometers across, so
  small that they are difficult to see under even
  the best light microscopes
• In contrast, the giant amoeba Chaos chaos may be
  1000 micrometers in diameter, large enough to be
  seen with the unaided eye as a tiny speck in pond
  water
• Despite their differences, all cells have two
  characteristics in common
• They are surrounded by a barrier called a cell
  membrane and, at some point in their lives, they
  contain the molecule that carries biological
  informationDNA

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Prokaryotes and Eukaryotes

• Cells fall into two broad categories, depending
  on whether they contain a nucleus
• The nucleus (plural nuclei) is a large
  membrane-enclosed structure that contains the
  cell's genetic material in the form of DNA
• A membrane is a thin layer of material that
  serves as a covering or lining
• The nucleus controls many of the cell's
  activities
• Eukaryotes are cells that contain nuclei
• Prokaryotes are cells that do not contain nuclei
• Both words derive from the Greek words karyon,
  meaning kernel, or nucleus, and eu, meaning
  true, or pro, meaning before
• These words reflect the idea that prokaryotic
  cells evolved before nuclei developed

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Prokaryotes 

• Prokaryotic cells are generally smaller and
  simpler than eukaryotic cells, although there are
  many exceptions to this rule
• Prokaryotic cells have genetic material that is
  not contained in a nucleus
• Some prokaryotes contain internal membranes, but
  prokaryotes are generally less complicated than
  eukaryotes
• NO MEMBRANE BOUND ORGANELLES
• Despite their simplicity, prokaryotes carry out
  every activity associated with living things
• They grow, reproduce, respond to the environment,
  and some can even move by gliding along surfaces
  or swimming through liquids
• The organisms we call bacteria are prokaryotes

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Eukaryotes 

• Eukaryotic cells are generally larger and more
  complex than prokaryotic cells
• Eukaryotic cells generally contain dozens of
  structures and internal membranes, and many are
  highly specialized
• MEMBRANE BOUND ORGANELLES
• Eukaryotic cells contain a nucleus in which their
  genetic material is separated from the rest of
  the cell
• Eukaryotes display great variety
• Some eukaryotes live solitary lives as
  single-celled organisms
• Others form large, multicellular organisms.
  Plants, animals, fungi, and protists are
  eukaryotes

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Cytoplasmic Organelles

• Little organs
• Specialized cellular compartments, each
  performing its own job to maintain the life of
  the cell
• Membranous organelles
• Bounded by a membrane similar in composition to
  the plasma membranre (minus the glycocalyx)
• This membrane enables them to maintain an
  internal environment different from that of the
  surrounding cytosol
• Examples
• Mitochondria
• Peroxisomes
• Lysosomes
• Endoplasmic reticulum
• Golgi apparatus
• Nonmembranous organelles
• Examples
• Cytoskeleton
• Centrioles
• Ribosomes

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Eukaryotic Cell Structure

• In some respects, the eukaryotic cell is like a
  factory
• The first time you look at a microscope image of
  a cell, the cell seems impossibly complex
• Look closely at a eukaryotic cell, however, and
  patterns begin to emerge
• To see those patterns more clearly, we'll look at
  some structures that are common to eukaryotic
  cells, shown in the figure at right
• Because many of these structures act as if they
  are specialized organs, these structures are
  known as organelles, literally little organs

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Eukaryotic Cell Structure
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Characteristics of Cells

• All cells have the same basic parts and some
  common functions
• A generalized human cell contains the plasma
  membrane, the cytoplasm, and the nucleus

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Eukaryotic Cell Structure

• Cell biologists divide the eukaryotic cell into
  two major parts
• Nucleus
• Cytoplasm
• The cytoplasm is the portion of the cell outside
  the nucleus
• As you will see, the nucleus and cytoplasm work
  together in the business of life

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Nucleus

• In the same way that the main office controls a
  large factory, the nucleus is the control center
  of the cell
• The nucleus contains nearly all the cell's DNA
  and with it the coded instructions for making
  proteins and other important molecules
• The structure of the nucleus is shown in the
  figure at right

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THE NUCLEUS

• The nucleus is the control center of the cell and
  contains the cellular DNA
• Most cells have only one nucleus, but very large
  cells may be multinucleate
• Presence of more than one nucleus usually
  signifies that a larger-than-usual cytoplasmic
  mass must be regulated
• All body cells except mature red blood cells
  (anucleate) have nuclei
• The nucleus is larger than the cytoplasmic
  organelles
• It has three regions
• Nuclear envelope (membrane)
• Nucleoli
• Chromatin

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Nucleus
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Nuclear Envelope

• The nucleus is surrounded by a nuclear envelope
  composed of two membranes
• The nuclear envelope is dotted with thousands of
  nuclear pores, which allow material to move into
  and out of the nucleus
• Like messages, instructions, and blueprints
  moving in and out of a main office, a steady
  stream of proteins, RNA, and other molecules move
  through the nuclear pores to and from the rest of
  the cell

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Nuclear Envelope

• Is a double-membrane barrier (separated by a
  fluid-filled space) surrounding the nucleus
• Outer membrane is continuous with the rough ER of
  the cytoplasm and is studded with ribosomes on
  its external face
• Inner membrane is lined by a network of protein
  filaments ( the nuclear lamina) that maintains
  the shape of the nucleus
• At various points, nuclear pores penetrate areas
  where the membranes of the nuclear envelope fuse
• A complex of proteins, called a pore complex,
  lines each nuclear pore and regulates passage of
  large particles into and out of the nucleus
• Like other cell membranes, the nuclear envelope
  is selectively permeable, but here passage of
  substances is much freer than elsewhere
• Protein molecules imported from the cytoplasm and
  RNA molecules exported from the nucleus pass
  easily through the relatively large pores
• The nuclear envelope encloses the fluid and
  solutes of the nucleus, the nucleoplasm

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Chromatin

• The granular material you can see in the nucleus
  is called chromatin
• Chromatin consists of DNA bound to protein
• Most of the time, chromatin is spread throughout
  the nucleus
• When a cell divides, however, chromatin condenses
  to form chromosomes
• These distinct, threadlike structures contain the
  genetic information that is passed from one
  generation of cells to the next

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Chromatin

• (a) Appears as a fine, unevenly stained network,
  but special techniques reveal it as a system of
  bumpy threads weaving their way through the
  nucleoplasm
• Is roughly half DNA, the genetic material of the
  cell, and half globular histone proteins
• Nucleosomes are the fundamental unit of
  chromatin, consisting of discus-shaped cores or
  clusters of eight histone proteins connected like
  beads on a string by a DNA molecule
• DNA winds around each nucleosome and continues on
  to the next cluster via linker DNA segments

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Chromatin

• Histones provide physical means for packing the
  very long DNA molecules in a compact, orderly
  way, they also play an important role in gene
  regulation
• In a nondividing cell, addition of phosphate or
  methyl groups to histone exposes different DNA
  segments, or genes, so that they can dictate the
  specifications for protein synthesis
• When a cell is preparing to divide, chromatin
  condenses into dense, rodlike chromosomes
• Chromosome compactness avoids entanglement and
  breakage of the delicate chromatin strands during
  the movements that occur during cell division

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Nucleolus

• Most nuclei also contain a small, dense region
  known as the nucleolus
• The nucleolus is where the assembly of ribosomes
  begins

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Nucleoli

• Dark-staining spherical bodies within the nucleus
• NOT membrane bound
• There are typically one or two nucleoli per
  nucleus, but there may be more
• Site of the assembly of ribosomal subunits
• Therefore, large in actively growing cells that
  are making large amounts of tissue proteins

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Nucleus
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NUCLEUS
The nucleus is surrounded by a double membrane
called the nuclear envelope. Inside the envelope
is chromatin (combo of DNA and protein) which
will become chromosomes. The nucleolus is the
site where ribosomes are synthesized and
partially assembled. The nucleus is porous and
is the site where our genetic information is
held.
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Endoplasmic Reticulum

• Eukaryotic cells also contain an internal
  membrane system known as the endoplasmic
  reticulum, or ER, as shown in the figure at right
• The endoplasmic reticulum is the site where lipid
  components of the cell membrane are assembled,
  along with proteins and other materials that are
  exported from the cell

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Endoplasmic Reticulum
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Ribosomes

• One of the most important jobs carried out in the
  cellular factory is making proteins
• Proteins are assembled on ribosomes
• Ribosomes are small particles of RNA and protein
  found throughout the cytoplasm
• They produce proteins by following coded
  instructions that come from the nucleus
• Each ribosome, in its own way, is like a small
  machine in a factory, turning out proteins on
  orders that come from its bossthe cell nucleus
• Cells that are active in protein synthesis are
  often packed with ribosomes

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Ribosomes

• (a)Small staining granules consisting of protein
  and ribosomal RNA
• Each ribosome has two globular subunits that fit
  together
• Site of protein synthesis

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Ribosome
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Ribosomes

• Some float freely in the cytoplasm
• Make soluble proteins that function in the
  cytosol
• Some are attached to membranes, forming a complex
  called the rough endoplasmic reticulum
• Synthesize proteins destined either for
  incorporation into cell membranes or for export
  from the cell
• Ribosomes can switch back-and-forth between the
  two types

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Endoplasmic Reticulum

• The portion of the ER involved in the synthesis
  of proteins is called rough endoplasmic
  reticulum, or rough ER
• It is given this name because of the ribosomes
  found on its surface
• Newly made proteins leave these ribosomes and are
  inserted into the rough ER, where they may be
  chemically modified

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Endoplasmic Reticulum

• Proteins that are released, or exported, from the
  cell are synthesized on the rough ER, as are many
  membrane proteins
• Rough ER is abundant in cells that produce large
  amounts of protein for export
• Other cellular proteins are made on free
  ribosomes, which are not attached to membranes

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ER PROTEIN SYNTHESIS
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Endoplasmic Reticulum

• The other portion of the ER is known as smooth
  endoplasmic reticulum (smooth ER) because
  ribosomes are not found on its surface
• In many cells, the smooth ER contains collections
  of enzymes that perform specialized tasks,
  including the synthesis of membrane lipids and
  the detoxification of drugs
• Liver cells, which play a key role in detoxifying
  drugs, often contain large amounts of smooth ER

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Endoplasmic reticulum

• Is an extensive system of interconnected tubes
  and parallel membranes enclosing fluid-filled
  cavities, called cisternae, that coils and twist
  throughout the cytosol
• Continuous with the nuclear membrane
• Two varieties
• Rough ER
• Smooth ER

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ENDOPLASMIC RETICULUM
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Golgi Apparatus

• Proteins produced in the rough ER move next into
  an organelle called the Golgi apparatus,
  discovered by the Italian scientist Camillo Golgi
• As you can see in the figure at right, Golgi
  appears as a stack of closely apposed membranes
• The function of the Golgi apparatus is to modify,
  sort, and package proteins and other materials
  from the endoplasmic reticulum for storage in the
  cell or secretion outside the cell
• The Golgi apparatus is somewhat like a
  customization shop, where the finishing touches
  are put on proteins before they are ready to
  leave the factory
• From the Golgi apparatus, proteins are then
  shipped to their final destinations throughout
  the cell or outside of the cell

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Golgi Apparatus
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Golgi Apparatus

• Is a series of stacked, flattened, membranous
  sacs, shaped like hollow dinner plates,
  associated with swarms of tiny groups of
  membranous vesicles
• The main function of the Golgi apparatus is to
  modify, concentrate, and package the proteins and
  lipids made at the rough ER
• The transport vesicles that bud off from the
  rough ER move to and fuse with the membranes at
  its convex cis face (receiving side), of the
  Golgi apparatus

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GOLGI ROLE
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Lysosomes

• Even the neatest, cleanest factory needs a
  cleanup crew, and that's what lysosomes are
• Lysosomes are small organelles filled with
  enzymes
• One function of lysosomes is the digestion, or
  breakdown, of lipids, carbohydrates, and proteins
  into small molecules that can be used by the rest
  of the cell

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Lysosomes

• Lysosomes are also involved in breaking down
  organelles that have outlived their usefulness
• Lysosomes perform the vital function of removing
  junk that might otherwise accumulate and
  clutter up the cell
• A number of serious human diseases, including
  Tay-Sachs disease, can be traced to lysosomes
  that fail to function properly

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Lysosomes

• Spherical membranous organelles that contain
  digestive enzymes
• Abundant in phagocytes, the cells that dispose of
  invading bacteria and cell debris
• Digest almost all kinds of biological molecules
  functioning best in acidic environments
• Thus called acid hydrolases
• The lysosomal membrane is adapted to serve
  lysosomal functions in two important ways
• 1.Contains H (proton) pumps, ATPases that gather
  hydrogen ions from the surrounding cytosol to
  maintain the organelles acidic pH
• 2.It retains the dangerous acid hydrolases while
  permitting the final products of digestion to
  escape so that they can be used by the cell or
  excreted
• Hence, lysosomes provide sites where digestion
  can proceed safely within a cell

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LYSOSOMES
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LYSOSOMES

• Contains 40 different kinds of digestive enzymes
• Digest food particles, bacteria, and worn-out or
  broken cell parts
• Small, spherical organelles surrounded by a
  single membrane
• Exist primarily in animal and fungal cells
• Role in early (embryonic) development
• Enzymes selectively destroy tissue

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Lysosomes

• Function as a cells demolition crew
• Digesting particles taken in by endocytosis,
  particularly ingested bacteria, viruses, and
  toxins
• Degrading worn-out or nonfunctional organelles
• Performing metabolic functions, such as glycogen
  breakdown and release
• Breaking down nonuseful tissues, such as the webs
  between fingers and toes of a developing fetus
  and the uterine lining during menstruation
• Breaking down bone to release calcium ions into
  the blood

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Vacuoles

• Every factory needs a place to store things, and
  cells contain places for storage as well
• Some kinds of cells contain saclike structures
  called vacuoles that store materials such as
  water, salts, proteins, and carbohydrates
• In many plant cells there is a single, large
  central vacuole filled with liquid
• The pressure of the central vacuole in these
  cells makes it possible for plants to support
  heavy structures such as leaves and flowers

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Vacuoles

• Vacuoles are also found in some single-celled
  organisms and in some animals
• The paramecium contains a vacuole called a
  contractile vacuole
• By contracting rhythmically, this specialized
  vacuole pumps excess water out of the cell
• The control of water content within the cell is
  just one example of an important process known as
  homeostasis
• Homeostasis is the maintenance of a controlled
  internal environment

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VACUOLES

• Fluid-filled cavities or sacs
• Organelles often found in plants
• Store enzymes and waste products (like lysosomes
  in animal cells)
• Some of the waste products are toxic and need to
  be kept away from the rest of the cell
• In a mature cell, makes up about 90 of the volume

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VACUOLES
Vacuoles are a second common characteristic of
plant cells. These are fluid-filled organelles
that store enzymes and wastes.
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Mitochondria and Chloroplasts

• All living things require a source of energy
• Factories are hooked up to the local power
  company, but what about cells?
• Most cells get energy in one of two waysfrom
  food molecules or from the sun

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Mitochondria

• Nearly all eukaryotic cells, including plants,
  contain mitochondria (singular mitochondrion)
• Mitochondria are organelles that convert the
  chemical energy stored in food into compounds
  that are more convenient for the cell to use
• Mitochondria are enclosed by two membranesan
  outer membrane and an inner membrane
• The inner membrane is folded up inside the
  organelle

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Mitochondria

• One of the most interesting aspects of
  mitochondria is the way in which they are
  inherited
• In humans, all or nearly all of our mitochondria
  come from the cytoplasm of the ovum, or egg cell
• This means that when your relatives are
  discussing which side of the family should take
  credit for your best characteristics, you can
  tell them that you got your mitchondria from Mom!

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Mitochondria

• Sausage-shaped membranous organelle
• In living cells they squirm, elongate, and change
  shape almost continuously
• Power plants of the cell, providing most of its
  ATP supply
• Enclosed by two membranes, each with the general
  structure of the plasma membrane
• Outer membrane is smooth and featureless
• Inner membrane folds inward, forming shelflike
  cristae that protrude into the matrix, the
  gel-like substance within the mitochondrion
• Intermediate products of food fuels are broken
  down to water and carbon dioxide by teams of
  enzymes, some dissolved in the mitochondrial
  matrix and others forming part of the crista
  membrane

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MITOCHONDRION
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Mitochondria

• Site of aerobic respiration (requires oxygen)
• Contain their own DNA and RNA and are able to
  reproduce themselves
• Capable of fission
• Contain approximately 37 genes that direct the
  synthesis of some proteins required for
  mitochondrial functions
• Believed that mitochondria arose from bacteria
  that invaded the ancestors of plant and animal
  cells

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PLASTIDS

• Large organelles in plants
• Store food or pigments
• Organelle where solar energy is converted into
  chemical energy and stored
• Types
• Chloroplast
• Contains a green pigment (chlorophyll) that
  absorbs sunlight as the first step in
  photosynthesis
• Chromoplasts
• Synthesis and store pigments such as orange
  carotenes, yellow xanthophylls, and various red
  pigments, some of which function in trapping
  sunlight for energy
• Give certain plants their distinctive colors
• Leucoplasts
• Store food such as starches, proteins, and lipids

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Chloroplasts

• Plants and some other organisms contain
  chloroplasts
• Chloroplasts are organelles that capture the
  energy from sunlight and convert it into chemical
  energy in a process called photosynthesis
• Chloroplasts are the biological equivalents of
  solar power plants
• Like mitochondria, chloroplasts are surrounded by
  two membranes
• Inside the organelle are large stacks of other
  membranes, which contain the green pigment
  chlorophyll

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CHLOROPLAST
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Organelle DNA 

• Unlike other organelles that contain no DNA,
  chloroplasts and mitochondria contain their own
  genetic information in the form of small DNA
  molecules
• Lynn Margulis, an American biologist, has
  suggested that mitochondria and chloroplasts are
  actually the descendants of ancient prokaryotes
• Margulis suggests that the prokaryotic ancestors
  of these organelles evolved a symbiotic
  relationship with early eukaryotes, taking up
  residence within the eukaryotic cell
• One group of prokaryotes had the ability to use
  oxygen to generate ATP
• These prokaryotes evolved into mitochondria
• Other prokaryotes that carried out photosynthesis
  evolved into
• Chloroplasts
• This idea is called the endosymbiotic theory

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Cytoskeleton

• A supporting structure and a transportation
  system complete our picture of the cell as a
  factory
• As you know, a factory building is supported by
  steel or cement beams and by columns that support
  its walls and roof
• Eukaryotic cells have a structurethe
  cytoskeletonthat helps support the cell
• The cytoskeleton is a network of protein
  filaments that helps the cell to maintain its
  shape
• The cytoskeleton is also involved in movement
• Microfilaments and microtubules are two of the
  principal protein filaments that make up the
  cytoskeleton

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Cytoskeleton

• Series of rods running through the cytosol,
  supporting cellular structures and aiding in cell
  movement
• There are three types of rods in the
  cytoskeletonnot covered by membranes
• Microtubules
• Microfilaments
• Intermediate filaments

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Cytoskeleton

• The cytoskeleton is a network of protein
  filaments that helps the cell to maintain its
  shape and is involved in many forms of cell
  movement

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Microfilaments

• Microfilaments are threadlike structures made of
  a protein called actin
• They form extensive networks in some cells and
  produce a tough, flexible framework that supports
  the cell
• Microfilaments also help cells move
• Microfilament assembly and disassembly is
  responsible for the cytoplasmic movements that
  allow cells, such as amoebas, to crawl along
  surfaces

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Microfilaments

• Thinnest elements of the cytoskeleton
• Strands of the protein actin (ray)
• Each cell has its own unique arrangements (NO TWO
  CELLS ARE ALIKE)
• Nearly all cells have a fairly dense cross-linked
  network of microfilaments attached to the
  cytoplasmic side of their plasma membrane that
  strengthens the cell surface

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Microtubules

• Microtubules, as shown in the figure to the
  right, are hollow structures made up of proteins
  known as tubulins
• In many cells, they play critical roles in
  maintaining cell shape
• Microtubules are also important in cell division,
  where they form a structure known as the mitotic
  spindle, which helps to separate chromosomes
• In animal cells, tubulin is also used to form a
  pair of structures known as centrioles
• Centrioles are located near the nucleus and help
  to organize cell division
• Centrioles are not found in plant cells

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Microtubules

• Microtubules also help to build projections from
  the cell surface, which are known as cilia
  (singular cilium) and flagella (singular
  flagellum), that enable cells to swim rapidly
  through liquids
• Cilia and flagella can produce considerable
  force and in some cells they move almost like
  the oars of a boat, pulling or pushing cells
  through the water

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Microtubules

• Largest diameter
• Hollow tubes made of spherical protein subunits
  called tubulins
• Most radiate from a small region of cytoplasm
  near the nucleus called the centrosome
• Constantly growing from the centrosome,
  disassembling, and then reassembling

90
Cytoskeleton
91
Cell Boundaries

• When you first study a country, you may begin by
  examining a map of the country's borders
• Before you can learn anything about a nation,
  it's important to understand where it begins and
  where it ends
• The same principle applies to cells
• Among the most important parts of a cell are its
  borders, which separate the cell from its
  surroundings
• All cells are surrounded by a thin, flexible
  barrier known as the cell membrane
• Many cells also produce a strong supporting layer
  around the membrane known as a cell wall

92
Cell Membrane

• The cell membrane regulates what enters and
  leaves the cell and also provides protection and
  support
• The composition of nearly all cell membranes is a
  double-layered sheet called a lipid bilayer
• As you can see in the figure at right, there are
  two layers of lipids, hence the name bilayer
• The lipid bilayer gives cell membranes a flexible
  structure that forms a strong barrier between the
  cell and its surroundings

93
Cell Membrane

• In addition to lipids, most cell membranes
  contain protein molecules that are embedded in
  the lipid bilayer
• Carbohydrate molecules are attached to many of
  these proteins
• There are so many kinds of molecules in cell
  membranes that scientists describe the membrane
  as a mosaic of different molecules
• A mosaic is a work of art made of individual
  tiles or other pieces assembled to form a picture
  or design
• Some of the proteins form channels and pumps that
  help to move material across the cell membrane
• Many of the carbohydrates act like chemical
  identification cards, allowing individual cells
  to identify one another

94
The Fluid Mosaic Model

• The inward-facing and outward-facing surfaces of
  the plasma membrane differ in the kinds and
  amounts of lipids they contain
• The majority of membrane phospholipids are
  unsaturated (like phosphatidyl choline), a
  condition which kinks their tails (increasing the
  space between them) and increases fluidity
• Glycolipids, phospholipids with attached sugar
  groups, are found only in the outer membrane (5
  of membrane)
• Sugar group makes that end of the glycolipid
  molecule polar, whereas the fatty acid tails are
  nonpolar
• Cholesterol (20 of membrane) stabilizes the
  lipid membrane by wedging its platelike
  hydrocarbon rings between the phospholipid tails
  and restraining movement of the phospholipids
• Lipid rafts (20), dynamic assemblies of
  saturated phospholipids (which pack together
  tightly) associated with unique lipids called
  sphinolipids and lots of cholesterol are also
  found only in the outer membrane
• More stable and orderly and less fluid than the
  rest of the membrane
• Include or exclude specific proteins to various
  extents
• Assumed to function in cell signaling

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96
The Fluid Mosaic Model

• Two distinct populations of membrane proteins
• Integral
• Peripheral

97
Functions of Membrane Proteins

• Proteins make up about 50 of the plasma membrane
  by mass and are responsible for most of the
  specialized membrane functions
• Transport
• Enzymatic activity
• Receptors for signal transduction
• Intercellular joining
• Cell-cell recognition
• Attachment to the cytoskeleton and extracellular
  matrix (ECM)

98
Plasma Membrane Structure

• Plasma membrane (cell membrane) defines the
  extent of the cell, separating two of the bodys
  major fluid compartments
• Intracellular fluid within cells
• Extracellular fluid outside cells

99
Cell Membrane
100
The Fluid Mosaic Model

• Plasma membrane is composed of a double layer of
  phospholipids embedded with small amounts of
  cholesterol and proteins dispersed in it
• The phospolipid bilayer is composed of two layers
  of phospholipids lying tail to tail
• Polar head is charged and hydrophilic
  (hydrowater, philicloving)
• Exposed to water inside (intracellular) and
  outside (extracellular) the cell
• Attracted to water
• Nonpolar tail is made of two fatty acid chains
  and is hydrophobic (phobiahating)
• Avoid water
• Line up in the center of the membrane

101
Cell Walls

• Cell walls are present in many organisms,
  including plants, algae, fungi, and many
  prokaryotes
• Cell walls lie outside the cell membrane
• Most cell walls are porous enough to allow water,
  oxygen, carbon dioxide, and certain other
  substances to pass through easily
• The main function of the cell wall is to provide
  support and protection for the cell

102
Cell Walls

• Most cell walls are made from fibers of
  carbohydrate and protein
• These substances are produced within the cell and
  then released at the surface of the cell membrane
  where they are assembled to form the wall
• Plant cell walls are composed mostly of
  cellulose, a tough carbohydrate fiber
• Cellulose is the principal component of both wood
  and paper, so every time you pick up a sheet of
  paper, you are holding the stuff of cell walls in
  your hand

103
CELL WALL

• Plant cells
• Surround the cell membrane and helps support and
  protect the cell
• Rigid covering
• Made primarily of long chains of cellulose
  embedded in hardening compounds such as pectin
  and lignin
• Pores allow ions and molecules to pass to and
  from the cell membrane

104
CELL WALL

• Two types both give strength to the cell
• Primary
• Formed during cell growth
• Secondary
• Formed after growth has ceased

105
PLANT CELL DIVISIONCELL WALL FORMATION

• Middle lamella forms between the new cells
• Intercellular glue of pectin (substance that
  makes jelly gel)
• Primary wall is formed between the middle lamella
  and the cell membrane
• Cellulose fibers are laid down in layers (all of
  the fibers in one direction)
• Growing cell is thus able to expand sequentially
  at a right angle to the most recent fiber layer
• Functions both to protect and allow the cell to
  grow
• Secondary wall forms after growth of the primary
  wall ceases
• Made of cellulose and lignin (makes wall woody)
  fibers interwoven so that no further expansion of
  the cell is possible
• Functions to strengthen the mature cell
• When you hold a piece of wood, you are holding
  secondary cell walls

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107
Diffusion Through Cell Boundaries

• Every living cell exists in a liquid environment
  that it needs to survive
• It may not always seem that way yet even in the
  dust and heat of a desert, the cells of cactus
  plants, scorpions, and vultures are bathed in
  liquid
• One of the most important functions of the cell
  membrane is to regulate the movement of dissolved
  molecules from the liquid on one side of the
  membrane to the liquid on the other side

108
Measuring Concentration 

• The cytoplasm of a cell contains a solution of
  many different substances in water
• Recall that a solution is a mixture of two or
  more substances
• The substances dissolved in the solution are
  called solutes
• The concentration of a solution is the mass of
  solute in a given volume of solution, or
  mass/volume
• Example
• If you dissolved 12 grams of salt in 3 liters of
  water, the concentration of the solution would be
  12 g/3 L, or 4 g/L (grams per liter)
• If you had 12 grams of salt in 6 liters of water,
  the concentration would be 12 g/6 L, or 2 g/L
• The first solution is twice as concentrated as
  the second solution

109
Diffusion

• In a solution, particles move constantly
• They collide with one another and tend to spread
  out randomly
• As a result, the particles tend to move from an
  area where they are more concentrated to an area
  where they are less concentrated, a process known
  as diffusion
• When the concentration of the solute is the same
  throughout a system, the system has reached
  equilibrium

110
Passive ProcessDiffusion

• Diffusion is a process in which substances
  scatter evenly throughout the environment from an
  area of higher concentration to an area of lower
  concentration
• Molecules move randomly, collide and ricochet off
  one another, changing direction with each
  collision
• Overall effect of this erratic movement is that
  molecules or ions move away from areas where they
  are in higher concentration to areas where their
  concentration is lower, so we say that molecule
  diffuse along, or down, their concentration
  gradient until equilibrium
• The greater the difference in concentration
  between the two areas, the faster the net
  diffusion of the particles

111
Passive ProcessDiffusion

• Driving force is the kinetic energy of the
  molecules themselves
• The speed is influenced by
• Molecular size
• Smaller the faster
• Temperature
• Warmer, the faster
• Example
• Peeling an onion, releases volatile substances
  that diffuse through the air, dissolving in the
  fluid film covering your eyes forming irritating
  sulfuric acid

112
Diffusion

• What do diffusion and equilibrium have to do with
  cell membranes?
• Suppose a substance is present in unequal
  concentrations on either side of a cell membrane,
  as shown in the figure at right
• If the substance can cross the cell membrane, its
  particles will tend to move toward the area where
  it is less concentrated until equilibrium is
  reached
• At that point, the concentration of the substance
  on both sides of the cell membrane will be the
  same

113
Diffusion

• Because diffusion depends upon random particle
  movements, substances diffuse across membranes
  without requiring the cell to use energy
• Even when equilibrium is reached, particles of a
  solution will continue to move across the
  membrane in both directions
• However, because almost equal numbers of
  particles move in each direction, there is no
  further change in concentration

114
Diffusion
115
Passive ProcessSimple Diffusion

• Nonpolar and lipid-soluble substances diffuse
  directly through the lipid bilayer
• Examples oxygen, carbon dioxide, fat-soluble
  vitamins, and alcohol

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Osmosis

• Although many substances can diffuse across
  biological membranes, some are too large or too
  strongly charged to cross the lipid bilayer
• If a substance is able to diffuse across a
  membrane, the membrane is said to be permeable to
  it
• A membrane is impermeable to substances that
  cannot pass across it
• Most biological membranes are selectively
  permeable, meaning that some substances can pass
  across them and others cannot

117
Osmosis

• Water passes quite easily across most membranes,
  even though many solute molecules cannot
• An important process known as osmosis is the
  result
• Osmosis is the diffusion of water through a
  selectively permeable membrane

118
How Osmosis Works 

• Look at the beaker on the left in the figure at
  right
• There are more sugar molecules on the left side
  of the selectively permeable membrane than on the
  right side
• That means that the concentration of water is
  lower on the left than it is on the right
• The membrane is permeable to water but not to
  sugar
• This means that water can cross the membrane in
  both directions, but sugar cannot
• As a result, there is a net movement of water
  from the area of high concentration to the area
  of low concentration

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How Osmosis Works
120
Osmolarity

• When equal volumes of aqueous solutions of
  different osmolarity are separated by a membrane
  that is permeable to all molecules in the system,
  net diffusion of both solute and water occurs,
  each moving down its own concentration gradient
• Eventually, equilibrium is reached when the water
  concentration on the left equals that on the
  right, and the solute concentration on both sides
  is the same

121
How Osmosis Works

• Water will tend to move across the membrane to
  the left until equilibrium is reached
• At that point, the concentrations of water and
  sugar will be the same on both sides of the
  membrane
• When this happens, the two solutions will be
  isotonic, which means same strength
• When the experiment began, the more concentrated
  sugar solution was hypertonic, which means above
  strength, as compared to the dilute sugar
  solution
• The dilute sugar solution was hypotonic, or
  below strength

122
Osmolarity

• If we consider the same system, but make the
  membrane impermeable to solute molecules, we see
  quite a different result
• Water quickly diffuses from the left to the right
  compartment and continues to do so until its
  concentration is the same on the two sides of the
  membrane
• Notice that in this case equilibrium results from
  the movement of WATER ALONE (the solutes are
  prevented from moving)
• Also the movement of water leads to dramatic
  changes in the volumes of the two compartments

123
Osmotic Pressure 

• For organisms to survive, they must have a way to
  balance the intake and loss of water
• Osmosis exerts a pressure known as osmotic
  pressure on the hypertonic side of a selectively
  permeable membrane
• Osmotic pressure can cause serious problems for a
  cell
• Because the cell is filled with salts, sugars,
  proteins, and other molecules, it will almost
  always be hypertonic to fresh water
• This means that osmotic pressure should produce a
  net movement of water into a typical cell that is
  surrounded by fresh water
• If that happens, the volume of a cell will
  increase until the cell becomes swollen
• Eventually, the cell may burst like an
  overinflated balloon
• The effects of osmosis are shown in the figure at
  right

124
Osmotic Pressure
125
Osmotic Pressure

• Fortunately, cells in large organisms are not in
  danger of bursting
• Most cells in such organisms do not come in
  contact with fresh water
• Instead, the cells are bathed in fluids, such as
  blood, that are isotonic
• These isotonic fluids have concentrations of
  dissolved materials roughly equal to those in the
  cells themselves

126
Osmotic Pressure

• Other cells, such as plant cells and bacteria,
  which do come into contact with fresh water, are
  surrounded by tough cell walls
• The cell walls prevent the cells from expanding,
  even under tremendous osmotic pressure
• However, the increased osmotic pressure makes the
  cells extremely vulnerable to injuries to their
  cell walls

127
Facilitated Diffusion

• A few molecules, such as the sugar glucose, seem
  to pass through the cell membrane much more
  quickly than they should
• One might think that these molecules are too
  large or too strongly charged to cross the
  membrane, and yet they diffuse across quite
  easily

128
Passive ProcessFacilitated Diffusion

• In facilitated diffusion substances are moved
  through, even though they are unable to pass
  through the lipid bilayer of the plasma membrane,
  by either
• Binding to protein carriers in the membrane
• Moving through channels
• Examples glucose and other sugars, amino acids,
  and ions

129
Facilitated Diffusion

• How does this happen?
• The answer is that cell membranes have protein
  channels that make it easy for certain molecules
  to cross the membrane
• Red blood cells, for example, have a cell
  membrane protein with an internal channel that
  allows glucose to pass through it
• Only glucose can pass through this channel, and
  it can move through in either direction
• This cell membrane protein is said to facilitate,
  or help, the diffusion of glucose across the
  membrane
• The process, shown to the right, is known as
  facilitated diffusion
• Hundreds of different protein channels have been
  found that allow particular substances to cross
  different membranes

130
Facilitated Diffusion

• During facilitated diffusion, molecules, such as
  glucose, that cannot diffuse across the cell
  membrane's lipid bilayer on their own move
  through protein channels instead

131
Facilitated Diffusion

• Although facilitated diffusion is fast and
  specific, it is still diffusion
• Therefore, a net movement of molecules across a
  cell membrane will occur only if there is a
  higher concentration of the particular molecules
  on one side than on the other side
• This movement does not require the use of the
  cell's energy

132
Facilitated DiffusionCarriers

• Is a transmembrane integral protein (sometimes
  called a permease) that shows specificity for
  molecules of a certain polar substance or class
  of substances that are too large to pass through
  membrane channels
• Examples sugars and amino acids
• Mechanism Carrier-Mediated Facilitated Diffusion
• Changes in shape of the carrier allow it to first
  envelop and then release the transported
  substance, shielding it en route from the
  nonpolar regions of the membrane
• Essentially, the binding site is moved from one
  face of the membrane to the other by changes in
  the conformation of the carrier protein

133
Facilitated DiffusionChannels

• Transmembrane proteins that serve to transport
  substances, usually ions or water, through
  aqueous channels from one side of the membrane to
  the other
• Types of Channels are
• Open Channels
• Are always open (leakage channels) and simply
  allow ion or water fluxes according to
  concentration gradients
• Gated and Controlled Channels
• Gated Binding or association sites exist within
  the channel and the channel is selective due to
  pore size and the charges of the amino acids
  lining the channel
• Controlled open or close by various chemical or
  electrical signals

134
Facilitated Diffusion
135
Integral Membrane Proteins

• Firmly inserted into the plasma membrane
• Some protrude from one membrane face only, BUT
  most are transmembrane proteins that span the
  entire width of the membrane and protrude on BOTH
  sides
• All have BOTH hydrophobic and hydrophilic
  regions
• This allows them to interact BOTH with the
  nonpolar lipid tails buried in the membrane and
  with water inside and outside the cell

136
Integral Membrane Proteins

• Mainly involved in transport
• Some cluster together to form channels, or pores,
  through which small, water-soluble molecules or
  ions can move, thus bypassing the lipid part of
  the membrane
• Some act as carriers that bind to a substance and
  then move it through the membrane
• Some are receptors for hormones or other chemical
  messengers and relay messages to the cell
  interior (process called signal transduction)

137
Active Transport

• As powerful as diffusion is, cells sometimes must
  move materials in the opposite directionagainst
  a concentration difference
• This is accomplished by a process known as active
  transport
• As its name implies, active transport requires
  energy
• The active transport of small molecules or ions
  across a cell membrane is generally carried out
  by transport proteins or pumps that are found
  in the membrane itself
• Larger molecules and clumps of material can also
  be actively transported across the cell membrane
  by processes known as endocytosis and exocytosis
• The transport of these larger materials sometimes
  involves changes in the shape of the cell
  membrane

138
Active Transport

• Similar to facilitated diffusion in that both
  require carrier proteins that combine
  specifically and reversibly with the transported
  substances
• HOWEVER, facilitated diffusion always honors
  concentration gradients because its driving force
  is kinetic energy
• IN CONTRAST, the active transporters or solute
  pumps move solutes, most importantly ions (such
  as Na, K, and Ca2), uphill against a
  concentration gradient
• To do this work, cells must expend the energy of
  ATP
• Very selective involving chemicals that cannot
  pass by diffusion
• Classified according to their energy source

139
Primary Active Transport

• Because Na and K leak slowly but continuously
  through channels in the plasma membrane along
  their concentration gradient (and cross rapidly
  in stimulated muscles and nerve cells), the
  Na-K pump operates more or less continuously as
  an antiport to simultaneously drive Na out of
  the cell against a steep concentration gradient
  and pump K back in

140
Molecular Transport Active Transport

• Small molecules and ions are carried across
  membranes by proteins in the membrane that act
  like energy-requiring pumps
• Many cells use such proteins to move calcium,
  potassium, and sodium ions across cell membranes
• Changes in protein shape, as shown in the figure
  at right, seem to play an important role in the
  pumping process
• A considerable portion of the energy used by
  cells in their daily activities is devoted to
  providing the energy to keep this form of active
  transport working.
• The use of energy in these systems enables cells
  to concentrate substances in a particular
  location, even when the forces of diffusion might
  tend to move these substances in the opposite
  direction

141
Molecular Transport Active Transport
142
Endocytosis  

• Larger molecules and even solid clumps of
  material may be transported by movements of the
  cell membrane
• One of these movements is called endocytosis
• Endocytosis is the process of taking material
  into the cell by means of infoldings, or pockets,
  of the cell membrane
• The pocket that results breaks loose from the
  outer portion of the cell membrane and forms a
  vacuole within the cytoplasm
• Large molecules, clumps of food, and even whole
  cells can be taken up in this way
• Two examples of endocytosis are phagocytosis and
  pinocytosis

143
ENDOCYTOSIS
144
Endocytosis Phagocytosis  

• Phagocytosis means cell eating
• In phagocytosis, extensions of cytoplasm surround
  a particle and package it within a food vacuole
• The cell then engulfs it
• Amoebas use this method of taking in food
• Engulfing material in this way requires a
  considerable amount of energy and, therefore, is
  correctly considered a form of active transport

145
Phagocytosis

• Type of endocytosis in which some relatively
  large or solid material, such as a clump of
  bacteria or cell debris, is engulfed by the cell
• Cytoplasmic extensions called pseudopods form and
  flow around the particle and engulf it
• Vesicle formed is called a phagosome
• In most cases, the phagosome then fuses with a
  lysosome and its contents are digested
• In humans, certain WBC and macrophages

146
Endocytosis Pinocytosis  

• In a process similar to endocytosis, many cells
  take up liquid from the surrounding environment
• Tiny pockets form along the cell membrane, fill
  with liquid, and pinch off to form vacuoles
  within the cell
• This process is known as pinocytosis

147
Pinocytosis

• Also called fluid-phase endocytosis (cell
  drinking)
• Bit of in