Probiotics

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Probiotics
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There are up to 400 different bacterial species
in the colon and the vast majority (99.9) are
strict anaerobes.

• Genus Log10 bacteria per gram
  feces
• Non-sporing anaerobes
• Bacteriodes spp. 10- 11
• Bifidobacterium spp. 10- 11
• Eubacterium spp. 9 - 11
• Propionibacterium spp. 9 - 11
• Veillonella spp. 5 - 8
• Sporing anaerobes
• Clostridium spp. 5 - 9
• Sporing aerobes
• Bacillus spp.
• Microaerophiles
• Lactobacillus spp. 7 - 9
• Streptococcus spp. 7 - 9
• Enterococci 5 - 7

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• For context Total Cells
• Them 100,000 billion.
• You 10,000 billion.

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Microbiotic therapy

• Indications for use
• Crohn's disease, ulcerative colitis, intestinal
  disturbances, gas and bloating, constipation,
  diarrhea, dysbiosis, yeast-overgrowth, parasites,
  irritable bowel syndrome, autoimmune conditions,
  MS, lupus, AIDS, rheumatoid arthritis, during and
  post antibiotic use, post chemotherapy, post
  radiation, food allergies, chronic fatigue
  syndrome and pharmaceutical drug use.

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The Big players

• Lactobacillus acidophilus
• Lactobacillus acidophilus is found primarily in
  the small intestine
• It produces natural antibiotics (lactocidin
  acidophilin), thereby, increasing your immune
  system's resistance against bad bacteria and
  fungus i.e. Candida albicans, Salmonella, E.
  coli, and Staphylococcus aureus
• It implants itself on the intestinal walls, the
  lining of the vagina, cervix and urethra
• For years it was assumed that Lactobacillus
  acidophilus was the most beneficial form of the
  good bacteria. However, recent research has
  revealed that Lactobacillus rhamnosus is just as
  important for good bacteria supplementation.
• Bifidobacterium bifidum
• Bifidobacterium bifidum reside mainly in the
  mucous membrane of the large intestine and
  vaginal tract
• Digests lactose
• Produces the most beneficial form of lactic acid
  and acetic acid
• Ferments indigestible fibers which produces more
  energy
• Synthesizes some vitamins and aids mineral
  absorption (calcium, magnesium and zinc)
• Inhibits the growth of Salmonella sp., Listeria,
  Shigella, E. coli and Clostridium per fringens by
  crowding out these bad bacteria and eating their
  nutrients
• Fights the bad bacteria in your body by lowering
  the intestinal pH through production of fatty
  acids
• Absorbs large quantities of ferrous ions,
  inhibiting the growth of bad bacteria
• Aids constipation and diarrhea with lactic acid
  production
• Lactobacillus rhamnosus 
• Lactobacillus rhamnosus is a healthier species of
  good bacteria and colonizes in your gut mucosa

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More Players

• Lactobacillus plantarum
• Helps produce lactolin, a natural antibiotic
• Synthesizes the amino acid, L-lysine, which is
  anti-viral
• Bifidobacterium infantis
• Present in the intestines of infants and adults
• Present in the vaginal tract
• Lactobacillus salivarius
• Destroys harmful bacteria
• Aids in bowel disorders
• Lactobacillus bulgaricus
• Used in fermenting natural yogurt
• Produces lactic acid
• Provides a great environment for Lactobacilli and
  Bifidobacterium to grow
• Lactobacillus casei
• Close relative to Lactobacillus acidophilus

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• Prebiotics?

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Fructo-oligo-saccharides

• Fructo-Oligo-Saccharides (FOS) is a
  naturally-occurring water-soluble fiber from
  fruit and vegetables which feeds the friendly
  intestinal bacteria.
• FOS generaly ensure rapid establishment of the
  Lactobacilli in the upper small intestine and
  bifido bacteria in the lower small intestine.
• Some, research suggests that there may be health
  benefits of supplementing the diet with between 5
  and l0g of FOS,
• It, HMF product, also provides naturally-occurring
  beta-carotene from the whole freeze-dried
  apricot. The apricot is used for its soothing
  effect on the intestinal walls (and great flavor
  in the powders).
• Townsend letter article http//www.findarticles.co
  m/p/articles/mi_m0ISW/is_2003_June/ai_102372155

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Inulin

• Inulin
• Inulin is a multi-chain fructooligosaccharide
  derived from chicory root
• It is a chain of fructose (a carbohydrate) that
  is not digestible by your body, therefore, it can
  pass through the body into the large intestine
  where it can feed your good bacteria cells
• Some, research has suggested that Inulin (9 chain
  links) is more effective than FOS (3 to 4 chain
  links) at feeding your body's good bacteria.
  Also, Inulin may be better tolerated by humans
  and bad bacteria are less likely to feed on it.

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• Human Micro Flora HMF

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Genestra Brands Form SeroyalNew! Oct 2004, all
HMF probiotics contain a substantial increase in
potency (double) and no price increase.

• HMF Replete contains
• Lactobacillus acidophilus
• Lactobacillus salivarius
• Bifidobacterium bifidum
• Bifidobacterium lactis
• Fructo-oligosaccharides
• high concentrations ensure rapid establishment
  and dominance of probiotic cultures in the GI
• Indicated during and following antibiotic
  therapy and chemotherapy. 

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Genestra Brands

• HMF Replete new and improved formula!
• Each sachet (20 g) of HMF Replete supplies
• Lactobacillus acidophilus .........75 billion CFU
• Bifidobacterium Bifidum .......12.5 billion CFU
• Bifidobacterium lactis ........... 12.5 billion
  CFU
• Lactobacillus salivarius ........... 30 billion
  CFU
• Fructo-oligosaccharides .................... 10 g
• May contain up to .................. 170 billion
  CFU

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HMF Recommendations

• Intensive Phase
• Take 1 packet daily (½ packet twice daily) after
  meals, mixed in water or off the spoon for 7-14
  days.
• Clinical Considerations
• During and Post-antibiotic therapy
• Acute intestinal upset
• Candidiasis
• Digestive disturbances (bloating, gas)
• Parasitic infections
• Chronic Fatigue Syndrome
• Bowel problems (constipation, diarrhea, IBS)

• Intermediate Phase
• Take ½ packet daily after meals mixed in water or
  off the spoon.
• Clinical Considerations
• General illness
• Stressful life style
• Parasitic infections
• Digestive problems (bloating, gas)
• Constipation
• Excessive alcohol consumption
• Bowel problems (constipation, diarrhea, IBS)
• Chronic Fatigue Syndrome
• Candidiasis

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Genestra Brands

• HMF Replete Features
• HMF replete is the strongest HMF probiotic
• prolonged stability allowing for storage up to 3
  months at room temperature
• resistant to stomach acidity and bile acids
• scientific evidence with clinical data to confirm
  efficacy
• laboratory tested to confirm potency and purity
• exceptional ability to attach to intestinal cells
  due to human sourced lactobacilli and
  bifidobacteria

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Genestra Brands

• Super Powder is a high-level maintenance
  probiotic formula.
• Indicated for individuals who use antibiotics,
  oral contraceptives, steroids and hormonal
  replacement therapy, and for extreme fatigue,
  allergies, yeast overgrowth, stress,
  hypercholesterolemia and indigestion. 
• HMF Super Powder is highly recommended for
  ongoing maintenance in individuals who are
  immuno-compromised.
• Ideal for children and adults who dislike or
  have difficulty swallowing capsules. 
• Powder is ideal for vegans

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Genestra Brands

• Each 1/4 teaspoon (1 gram) of HMF Super Powder
  supplies
• Lactobacillus acidophilus (2 strains) ....... 6
  billion CFU
• Bifidobacterium bifidum .........................
  1 billion CFU
• Bifidobacterium lactis ...........................
  .. 1 billion CFU
• Fructo-oligosaccharides ..........................
  ...... 800 mg
• May contain up to ................................
  . 12 billion CFU

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• Intensive Phase Take ½ teaspoon twice daily
  after meals for one month. Please note, for more
  severe intestinal upsets you may safely take 1
  Tablespoon twice daily.
• Clinical Considerations
• During and post-antibiotic use
• Acute intestinal upset (food poisoning, stomach
  ache)
• Digestive disturbances (bloating, gas)
• Candidiasis (yeast infections)
• Bacterial Vaginosis
• Parasitic infections
• Chronic Fatigue Syndrome
• Bowel problems (constipation, diarrhea, IBS)

• Intermediate PhaseTake ¼ teaspoon one or twice
  daily for one month.
• Clinical Considerations
• General illness (cold, flu, mild infection)
• Stressful life style
• Parasitic infections
• Digestive problems (bloating, gas)
• Constipation
• Excessive alcohol consumption
• Bowel problems (constipation, diarrhea, IBS)
• Chronic Fatigue Syndrome
• Candidiasis

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Genestra Brands

• Each vegetable capsule contains
• Lactobacillus acidophilus (two human derived
  strains)....... 6 billion CFU
• Bifidobacterium bifidum.......1 billion CFU
• Bifidobacterium lactis.......1 billion CFU
• Fructo-oligo-saccharides (FOS)....... 200 mg
• May contain up to.......12 billion CFUAll HMF
  probiotics are gluten and casein free.

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Genestra Brands Forte Tx recommendations

• Intermediate Phase Take 4 capsules daily (2 caps
  twice daily after meals)
• Clinical Considerations
• General illness
• Stressful life style
• Parasitic infections
• Digestive problems (bloating, gas)
• Constipation
• Excessive alcohol consumption
• Bowel problems (diarrhea, IBS)
• Chronic Fatigue Syndrome
• Candidiasis

• Maintenance Phase1 or more capsules daily after
  meals
• Clinical Considerations
• Maintain Health of the digestive system
• Prevention of colds and flu
• Stressful life style
• Digestive problems (bloating, gas)
• Occasional Constipation
• Prevention of Candidiasis yeast infections

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Genestra BrandsHMF Natogen (previously Neogen)

•  Ingredients and Suggested Use
• Each Scoop (100 mg) Contains
• Lactobacillus acidophilus, guaranteed 1 billion
  CFU
• Lactobacillus casei 1 billion CFU
• Bifidobacterium infantis May contain up to 4.5
  billion CFU
• Lactose base (an important energy source for
  breast and bottle fed babies derived from milk
  but free of casein)
• Recommended DosageInfants Add one level scoop
  daily into the feeding, or dip your baby finger
  into scoop and give to infant to suckle on
  throughout the day.All HMF probiotics are
  gluten and casein free.

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Genestra price list

• Probiotics Prebiotics
• HMF______________________ 60 caps _ 10.50
• HMF Forte _________________ 60 caps _ 15.00 HMF
  Forte ________________120 caps _ 27.50 HMF Neogen
  _________________ 6 g __ 10.50
• HMF Powder_________________ 60 g __ 13.25
• HMF Replete _______________ 7 sachets 17.25 HMF
  Super Powder ___________120 g __ 26.00
• FOS L-Glutamine___________ 60 caps _ 8.25

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Genestra Brands

• Seroyal, a Natural pharmaceutical company founded
  in 1984, manufactures and distributes the
  extensive line of Genestra Brands nutritional and
  botanical products and is the North American
  distributor for the renowned Unda Homeopathic
  line. Ask your registered practitioner for more
  information.
• Genestra Product Groups
• Amino Acids Proteins Antioxidants Oral
  Chelations Botanical Combinations Single
  Tinctures Calcium-Magnesium Complexes
  Digestives Probiotics Essential Fatty Acids
  Phospholipids Female Prenatal Formulas
  Homeopathic, Endocrine Glandular Extracts
  Minerals Glucosamines Standardized
  Phytonutrients Topical Care, Creams Clinical
  Aids Vitamin-Mineral Complexes Vitamins A, C, E
  B Complexltgt

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Genestra philosophy

• Our products are based on scientific rationale
  using only the purest, highest quality, natural,
  raw ingredients available. We work closely with
  you and your peers to incorporate the latest
  research and clinical findings into all our
  products.Seroyals products are made with care
  and attention to every detail. To ensure product
  uniformity, each ingredient is accurately
  weighed, tested and precisely mixed. To retain
  the full healing properties of each component,
  all tablets are cold-pressed and a low pressure
  compressing process is used. This unique method
  does not involve the use of heat as is the
  standard practice in the industry. No lubricants
  or fillers are used during our encapsulation
  process whenever possible. We use the purest
  source of lubricant available, such as
  biodegradable vegetable cellulose, to maintain
  consistent capsule weight and content conformity.
  Genestras vegetable capsules are free of
  pesticides, preservatives and additives, tested
  by government-approved independent laboratories
  to ensure quick dissolution and disintegration.
  We guarantee that the disintegration time does
  not exceed 20 minutes from the time of ingestion
  for capsules, and 30 minutes for all our tablets.
  We regularly test our capsules and tablets to
  confirm weight variation and ingredient
  potency.When manufacturing the Genestra
  products, we follow the procedures outlined in
  the Good Manufacturing Practices. We ensure
  precision and objectivity during testing by
  contracting government-approved independent
  laboratories to assay and verify each ingredient
  for every product in each lot. We reconfirm each
  ingredients potency, weight variation,
  disintegration time and the absence of heavy
  metals, bacteria, E. coli, salmonella, yeasts,
  mold and other contaminants.

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About Genestra Brands

• Are HMF products shipped with an ice pack?No.
  But, after extensive product testing and new
  freeze drying technology performed by Genestra,
  it was concluded that the ice pack did not make a
  difference during shipping since the product
  potency was still well over 100 label claims
  when shipped with ice or no ice. The manufacturer
  states that it is no longer necessary to ship the
  HMF products with an ice pack for these reasons
• Genestra has added in 50 more probiotic than
  what the label guarantees per bottle. For
  example, HMF Forte is guaranteed to have 6
  billion L. acidophilus per serving, but there are
  12 billion present at time of manufacturing and
  it is kept frozen until shipping.
• October 2004 The manufacturer has increased
  product stability again, allowing the probiotics
  to last up to 6 months at room temperature
  holding their GUARANTEED minimum potency on
  label!
• You will receive your HMF probiotic directly from
  the manufacturer in about 3-5 days. This product
  is very fresh and has not been sitting around on
  our shelves. And, you are guaranteed it will
  contain 50 more than what the label states.
• How do I travel with my HMF probiotics?We highly
  recommend you take your probiotics with you on
  all trips and vacations since their potency will
  last for up to 6 months at room temperature
  un-refrigerated. But, when possible and if
  convenient, we recommend you store your
  probiotics in the freezer or refrigerator,
  especially if you plan to preserve freshness of
  your product for months or years.

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What is Clostridium Difficile (C.difficile)?

• Clostridium difficile, or C. difficile (a
  gram-positive anaerobic, SPORE forming bacterium)
  
• Is now recognized as the major causative agent
  of colitis (inflammation of the colon) and
  diarrhea that may occur following antibiotic
  intake. C. difficile infection represents one of
  the most common hospital (nosocomial) infections
  around the world. Laboratory studies show that
  when C. difficile colonize the gut, they release
  two potent toxins, toxin A and toxin B, which
  bind to certain receptors in the lining of the
  colon and ultimately cause diarrhea and
  inflammation of the large intestine, or colon
  (colitis). This bacterium is primarily acquired
  in hospitals and chronic care facilities
  following antibiotic therapy (symptoms may be
  delayed until 6-8 weeks after antibiotic use). In
  addition to antibiotic use, chemotherapy and
  hospitalization, another risk factor for
  developing C.difficile diarrhea is simply
  advancing age. One study conducted in Sweden
  found that individuals older than 60 years of age
  are 20 to 100 times more likely to have
  C.difficile in their stools compared to 10-20
  year olds (Barlett, 2002).
• Symptoms of C.difficile?
• Symptoms of C.difficile diarrhea vary widely in
  severity. Some individuals experience recurrent
  mild-to-moderate diarrhea, wither others suffer
  from recurrent bouts of severe abdominal and back
  cramping, frequent loose and watery, greenish,
  foul-smelling stools, flatulence, fever, as well
  as variable amounts of mucus and blood in the
  stool. C.difficile diarrhea with accompanying
  inflammation of the colon can lead to toxic
  dilation of the colon, perforation of the colon,
  dehydration, loss of water volume, shock, and
  death. The toxins produced by C.difficile can
  also trigger a whole host of extra-intestinal
  complications, including joint pain and sleep
  disorders.

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C.Difficile Tx

• C.difficile be treated effectively?Although C.
  difficile infection usually responds to
  antibiotic treatment with metronidazole or
  vancomycin, approximately 20 to 25 of patients
  will experience re-appearance of diarrhea and
  other symptoms weeks or even months after initial
  therapy has been discontinued. When antibiotics
  are used to eradicate C.difficile, the
  antibiotics kill many of the good
  gastrointestinal flora species, but only kill
  non-spore forms of C.difficile. The C.difficile
  spores are still present. In order to eliminate
  C.difficile spores you need to have some good
  normal flora present. How can I re-establish my
  gastrointestinal flora to eliminate
  C.difficile?Numerous of studies have proven
  certain probiotics to be a successful solution
  for recurrent C.difficile diarrhea.

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Saccharomyces boulardii

• Saccharomyces boulardii
• A non-disease causing yeast that survives passage
  through the intestinal tract, unaffected by
  antibiotics (although it will be killed by
  antifungal drugs).
• Based on preliminary data indicating that this
  yeast could prevent antibiotic-associated
  diarrhea.
• Numerous studies, have gone on to prove
  Saccharomyces boulardii, as an effective
  treatment for active C.difficile diarrhea.
• Saccharomyces boulardii disrupts toxins that
  would normally irritate the intestines,
• In addition, Saccharomyces boulardii stimulates
  the intestinal immune system to secrete
  C.difficile toxin A-specific antibodies (IgA) in
  the gut (Qamar et al., 2001).
• It is then eliminated after supplementation is
  stopped.

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Allergy Research Group

• http//www.allergyresearchgroup.com/proddesc/categ
  ory/microbal.htm

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Thorne Lactobacillus sporogenes

• Lactobacillus sporogenes represents a
  breakthrough in Lactobacillus supplementation
• a safe, stable, effective, L()-lactic-acid-produc
  ing organism.
• Lactobacillus sporogenes binds cholesterol in the
  gut, and may inhibit the cholesterol-producing
  enzyme HMG CoA Reductase.
• It requires no refrigeration or special
  handling.

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Resources

• http//www.rockwellnutrition.net/home.asp
• http//www.gla.ac.uk/departments/humannutrition/st
  udents/resources/meden/Infection.pdf
• Manufacture sites
• http//www.seroyal.com/seroyal/Navigation/ProductL
  inePublic/Genestra_Line.htm
• http//www.allergyresearchgroup.com/proddesc/categ
  ory/microbal.htm
• http//www.thorne.com/altmedrev/fulltext/micro.htm
  l
• Microbial Ecology and Dysbiosis in Human
  Medicine Excellent Article

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• jefechi.com/probiotics