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Title: Probiotics

There are up to 400 different bacterial species
in the colon and the vast majority (99.9) are
strict anaerobes.
  • Genus Log10 bacteria per gram
  • Non-sporing anaerobes
  • Bacteriodes spp. 10- 11
  • Bifidobacterium spp. 10- 11
  • Eubacterium spp. 9 - 11
  • Propionibacterium spp. 9 - 11
  • Veillonella spp. 5 - 8
  • Sporing anaerobes
  • Clostridium spp. 5 - 9
  • Sporing aerobes
  • Bacillus spp.
  • Microaerophiles
  • Lactobacillus spp. 7 - 9
  • Streptococcus spp. 7 - 9
  • Enterococci 5 - 7

  • For context Total Cells
  • Them 100,000 billion.
  • You 10,000 billion.

Microbiotic therapy
  • Indications for use
  • Crohn's disease, ulcerative colitis, intestinal
    disturbances, gas and bloating, constipation,
    diarrhea, dysbiosis, yeast-overgrowth, parasites,
    irritable bowel syndrome, autoimmune conditions,
    MS, lupus, AIDS, rheumatoid arthritis, during and
    post antibiotic use, post chemotherapy, post
    radiation, food allergies, chronic fatigue
    syndrome and pharmaceutical drug use.

The Big players
  • Lactobacillus acidophilus
  • Lactobacillus acidophilus is found primarily in
    the small intestine
  • It produces natural antibiotics (lactocidin
    acidophilin), thereby, increasing your immune
    system's resistance against bad bacteria and
    fungus i.e. Candida albicans, Salmonella, E.
    coli, and Staphylococcus aureus
  • It implants itself on the intestinal walls, the
    lining of the vagina, cervix and urethra
  • For years it was assumed that Lactobacillus
    acidophilus was the most beneficial form of the
    good bacteria. However, recent research has
    revealed that Lactobacillus rhamnosus is just as
    important for good bacteria supplementation.
  • Bifidobacterium bifidum
  • Bifidobacterium bifidum reside mainly in the
    mucous membrane of the large intestine and
    vaginal tract
  • Digests lactose
  • Produces the most beneficial form of lactic acid
    and acetic acid
  • Ferments indigestible fibers which produces more
  • Synthesizes some vitamins and aids mineral
    absorption (calcium, magnesium and zinc)
  • Inhibits the growth of Salmonella sp., Listeria,
    Shigella, E. coli and Clostridium per fringens by
    crowding out these bad bacteria and eating their
  • Fights the bad bacteria in your body by lowering
    the intestinal pH through production of fatty
  • Absorbs large quantities of ferrous ions,
    inhibiting the growth of bad bacteria
  • Aids constipation and diarrhea with lactic acid
  • Lactobacillus rhamnosus 
  • Lactobacillus rhamnosus is a healthier species of
    good bacteria and colonizes in your gut mucosa

More Players
  • Lactobacillus plantarum
  • Helps produce lactolin, a natural antibiotic
  • Synthesizes the amino acid, L-lysine, which is
  • Bifidobacterium infantis
  • Present in the intestines of infants and adults
  • Present in the vaginal tract
  • Lactobacillus salivarius
  • Destroys harmful bacteria
  • Aids in bowel disorders
  • Lactobacillus bulgaricus
  • Used in fermenting natural yogurt
  • Produces lactic acid
  • Provides a great environment for Lactobacilli and
    Bifidobacterium to grow
  • Lactobacillus casei
  • Close relative to Lactobacillus acidophilus

  • Prebiotics?

  • Fructo-Oligo-Saccharides (FOS) is a
    naturally-occurring water-soluble fiber from
    fruit and vegetables which feeds the friendly
    intestinal bacteria.
  • FOS generaly ensure rapid establishment of the
    Lactobacilli in the upper small intestine and
    bifido bacteria in the lower small intestine.
  • Some, research suggests that there may be health
    benefits of supplementing the diet with between 5
    and l0g of FOS,
  • It, HMF product, also provides naturally-occurring
    beta-carotene from the whole freeze-dried
    apricot. The apricot is used for its soothing
    effect on the intestinal walls (and great flavor
    in the powders).
  • Townsend letter article http//

  • Inulin
  • Inulin is a multi-chain fructooligosaccharide
    derived from chicory root
  • It is a chain of fructose (a carbohydrate) that
    is not digestible by your body, therefore, it can
    pass through the body into the large intestine
    where it can feed your good bacteria cells
  • Some, research has suggested that Inulin (9 chain
    links) is more effective than FOS (3 to 4 chain
    links) at feeding your body's good bacteria.
    Also, Inulin may be better tolerated by humans
    and bad bacteria are less likely to feed on it.

  • Human Micro Flora HMF

Genestra Brands Form SeroyalNew! Oct 2004, all
HMF probiotics contain a substantial increase in
potency (double) and no price increase.
  • HMF Replete contains
  • Lactobacillus acidophilus
  • Lactobacillus salivarius
  • Bifidobacterium bifidum
  • Bifidobacterium lactis
  • Fructo-oligosaccharides
  • high concentrations ensure rapid establishment
    and dominance of probiotic cultures in the GI
  • Indicated during and following antibiotic
    therapy and chemotherapy. 

Genestra Brands
  • HMF Replete new and improved formula!
  • Each sachet (20 g) of HMF Replete supplies
  • Lactobacillus acidophilus .........75 billion CFU
  • Bifidobacterium Bifidum .......12.5 billion CFU
  • Bifidobacterium lactis ........... 12.5 billion
  • Lactobacillus salivarius ........... 30 billion
  • Fructo-oligosaccharides .................... 10 g
  • May contain up to .................. 170 billion

HMF Recommendations
  • Intensive Phase
  • Take 1 packet daily (½ packet twice daily) after
    meals, mixed in water or off the spoon for 7-14
  • Clinical Considerations
  • During and Post-antibiotic therapy
  • Acute intestinal upset
  • Candidiasis
  • Digestive disturbances (bloating, gas)
  • Parasitic infections
  • Chronic Fatigue Syndrome
  • Bowel problems (constipation, diarrhea, IBS)
  • Intermediate Phase
  • Take ½ packet daily after meals mixed in water or
    off the spoon.
  • Clinical Considerations
  • General illness
  • Stressful life style
  • Parasitic infections
  • Digestive problems (bloating, gas)
  • Constipation
  • Excessive alcohol consumption
  • Bowel problems (constipation, diarrhea, IBS)
  • Chronic Fatigue Syndrome
  • Candidiasis

Genestra Brands
  • HMF Replete Features
  • HMF replete is the strongest HMF probiotic
  • prolonged stability allowing for storage up to 3
    months at room temperature
  • resistant to stomach acidity and bile acids
  • scientific evidence with clinical data to confirm
  • laboratory tested to confirm potency and purity
  • exceptional ability to attach to intestinal cells
    due to human sourced lactobacilli and

Genestra Brands
  • Super Powder is a high-level maintenance
    probiotic formula.
  • Indicated for individuals who use antibiotics,
    oral contraceptives, steroids and hormonal
    replacement therapy, and for extreme fatigue,
    allergies, yeast overgrowth, stress,
    hypercholesterolemia and indigestion. 
  • HMF Super Powder is highly recommended for
    ongoing maintenance in individuals who are
  • Ideal for children and adults who dislike or
    have difficulty swallowing capsules. 
  • Powder is ideal for vegans

Genestra Brands
  • Each 1/4 teaspoon (1 gram) of HMF Super Powder
  • Lactobacillus acidophilus (2 strains) ....... 6
    billion CFU
  • Bifidobacterium bifidum .........................
    1 billion CFU
  • Bifidobacterium lactis ...........................
    .. 1 billion CFU
  • Fructo-oligosaccharides ..........................
    ...... 800 mg
  • May contain up to ................................
    . 12 billion CFU

  • Intensive Phase Take ½ teaspoon twice daily
    after meals for one month. Please note, for more
    severe intestinal upsets you may safely take 1
    Tablespoon twice daily.
  • Clinical Considerations
  • During and post-antibiotic use
  • Acute intestinal upset (food poisoning, stomach
  • Digestive disturbances (bloating, gas)
  • Candidiasis (yeast infections)
  • Bacterial Vaginosis
  • Parasitic infections
  • Chronic Fatigue Syndrome
  • Bowel problems (constipation, diarrhea, IBS)
  • Intermediate PhaseTake ¼ teaspoon one or twice
    daily for one month.
  • Clinical Considerations
  • General illness (cold, flu, mild infection)
  • Stressful life style
  • Parasitic infections
  • Digestive problems (bloating, gas)
  • Constipation
  • Excessive alcohol consumption
  • Bowel problems (constipation, diarrhea, IBS)
  • Chronic Fatigue Syndrome
  • Candidiasis

Genestra Brands
  • Each vegetable capsule contains
  • Lactobacillus acidophilus (two human derived
    strains)....... 6 billion CFU
  • Bifidobacterium bifidum.......1 billion CFU
  • Bifidobacterium lactis.......1 billion CFU
  • Fructo-oligo-saccharides (FOS)....... 200 mg
  • May contain up to.......12 billion CFUAll HMF
    probiotics are gluten and casein free.

Genestra Brands Forte Tx recommendations
  • Intermediate Phase Take 4 capsules daily (2 caps
    twice daily after meals)
  • Clinical Considerations
  • General illness
  • Stressful life style
  • Parasitic infections
  • Digestive problems (bloating, gas)
  • Constipation
  • Excessive alcohol consumption
  • Bowel problems (diarrhea, IBS)
  • Chronic Fatigue Syndrome
  • Candidiasis
  • Maintenance Phase1 or more capsules daily after
  • Clinical Considerations
  • Maintain Health of the digestive system
  • Prevention of colds and flu
  • Stressful life style
  • Digestive problems (bloating, gas)
  • Occasional Constipation
  • Prevention of Candidiasis yeast infections

Genestra BrandsHMF Natogen (previously Neogen)
  •  Ingredients and Suggested Use
  • Each Scoop (100 mg) Contains
  • Lactobacillus acidophilus, guaranteed 1 billion
  • Lactobacillus casei 1 billion CFU
  • Bifidobacterium infantis May contain up to 4.5
    billion CFU
  • Lactose base (an important energy source for
    breast and bottle fed babies derived from milk
    but free of casein)
  • Recommended DosageInfants Add one level scoop
    daily into the feeding, or dip your baby finger
    into scoop and give to infant to suckle on
    throughout the day.All HMF probiotics are
    gluten and casein free.

Genestra price list
  • Probiotics Prebiotics
  • HMF______________________ 60 caps _ 10.50
  • HMF Forte _________________ 60 caps _ 15.00 HMF
    Forte ________________120 caps _ 27.50 HMF Neogen
    _________________ 6 g __ 10.50
  • HMF Powder_________________ 60 g __ 13.25
  • HMF Replete _______________ 7 sachets 17.25 HMF
    Super Powder ___________120 g __ 26.00
  • FOS L-Glutamine___________ 60 caps _ 8.25

Genestra Brands
  • Seroyal, a Natural pharmaceutical company founded
    in 1984, manufactures and distributes the
    extensive line of Genestra Brands nutritional and
    botanical products and is the North American
    distributor for the renowned Unda Homeopathic
    line. Ask your registered practitioner for more
  • Genestra Product Groups
  • Amino Acids Proteins Antioxidants Oral
    Chelations Botanical Combinations Single
    Tinctures Calcium-Magnesium Complexes
    Digestives Probiotics Essential Fatty Acids
    Phospholipids Female Prenatal Formulas
    Homeopathic, Endocrine Glandular Extracts
    Minerals Glucosamines Standardized
    Phytonutrients Topical Care, Creams Clinical
    Aids Vitamin-Mineral Complexes Vitamins A, C, E
    B Complexltgt

Genestra philosophy
  • Our products are based on scientific rationale
    using only the purest, highest quality, natural,
    raw ingredients available. We work closely with
    you and your peers to incorporate the latest
    research and clinical findings into all our
    products.Seroyals products are made with care
    and attention to every detail. To ensure product
    uniformity, each ingredient is accurately
    weighed, tested and precisely mixed. To retain
    the full healing properties of each component,
    all tablets are cold-pressed and a low pressure
    compressing process is used. This unique method
    does not involve the use of heat as is the
    standard practice in the industry. No lubricants
    or fillers are used during our encapsulation
    process whenever possible. We use the purest
    source of lubricant available, such as
    biodegradable vegetable cellulose, to maintain
    consistent capsule weight and content conformity.
    Genestras vegetable capsules are free of
    pesticides, preservatives and additives, tested
    by government-approved independent laboratories
    to ensure quick dissolution and disintegration.
    We guarantee that the disintegration time does
    not exceed 20 minutes from the time of ingestion
    for capsules, and 30 minutes for all our tablets.
    We regularly test our capsules and tablets to
    confirm weight variation and ingredient
    potency.When manufacturing the Genestra
    products, we follow the procedures outlined in
    the Good Manufacturing Practices. We ensure
    precision and objectivity during testing by
    contracting government-approved independent
    laboratories to assay and verify each ingredient
    for every product in each lot. We reconfirm each
    ingredients potency, weight variation,
    disintegration time and the absence of heavy
    metals, bacteria, E. coli, salmonella, yeasts,
    mold and other contaminants.

About Genestra Brands
  • Are HMF products shipped with an ice pack?No.
    But, after extensive product testing and new
    freeze drying technology performed by Genestra,
    it was concluded that the ice pack did not make a
    difference during shipping since the product
    potency was still well over 100 label claims
    when shipped with ice or no ice. The manufacturer
    states that it is no longer necessary to ship the
    HMF products with an ice pack for these reasons
  • Genestra has added in 50 more probiotic than
    what the label guarantees per bottle. For
    example, HMF Forte is guaranteed to have 6
    billion L. acidophilus per serving, but there are
    12 billion present at time of manufacturing and
    it is kept frozen until shipping.
  • October 2004 The manufacturer has increased
    product stability again, allowing the probiotics
    to last up to 6 months at room temperature
    holding their GUARANTEED minimum potency on
  • You will receive your HMF probiotic directly from
    the manufacturer in about 3-5 days. This product
    is very fresh and has not been sitting around on
    our shelves. And, you are guaranteed it will
    contain 50 more than what the label states.
  • How do I travel with my HMF probiotics?We highly
    recommend you take your probiotics with you on
    all trips and vacations since their potency will
    last for up to 6 months at room temperature
    un-refrigerated. But, when possible and if
    convenient, we recommend you store your
    probiotics in the freezer or refrigerator,
    especially if you plan to preserve freshness of
    your product for months or years.

What is Clostridium Difficile (C.difficile)?
  • Clostridium difficile, or C. difficile (a
    gram-positive anaerobic, SPORE forming bacterium)
  • Is now recognized as the major causative agent
    of colitis (inflammation of the colon) and
    diarrhea that may occur following antibiotic
    intake. C. difficile infection represents one of
    the most common hospital (nosocomial) infections
    around the world. Laboratory studies show that
    when C. difficile colonize the gut, they release
    two potent toxins, toxin A and toxin B, which
    bind to certain receptors in the lining of the
    colon and ultimately cause diarrhea and
    inflammation of the large intestine, or colon
    (colitis). This bacterium is primarily acquired
    in hospitals and chronic care facilities
    following antibiotic therapy (symptoms may be
    delayed until 6-8 weeks after antibiotic use). In
    addition to antibiotic use, chemotherapy and
    hospitalization, another risk factor for
    developing C.difficile diarrhea is simply
    advancing age. One study conducted in Sweden
    found that individuals older than 60 years of age
    are 20 to 100 times more likely to have
    C.difficile in their stools compared to 10-20
    year olds (Barlett, 2002).
  • Symptoms of C.difficile?
  • Symptoms of C.difficile diarrhea vary widely in
    severity. Some individuals experience recurrent
    mild-to-moderate diarrhea, wither others suffer
    from recurrent bouts of severe abdominal and back
    cramping, frequent loose and watery, greenish,
    foul-smelling stools, flatulence, fever, as well
    as variable amounts of mucus and blood in the
    stool. C.difficile diarrhea with accompanying
    inflammation of the colon can lead to toxic
    dilation of the colon, perforation of the colon,
    dehydration, loss of water volume, shock, and
    death. The toxins produced by C.difficile can
    also trigger a whole host of extra-intestinal
    complications, including joint pain and sleep

C.Difficile Tx
  • C.difficile be treated effectively?Although C.
    difficile infection usually responds to
    antibiotic treatment with metronidazole or
    vancomycin, approximately 20 to 25 of patients
    will experience re-appearance of diarrhea and
    other symptoms weeks or even months after initial
    therapy has been discontinued. When antibiotics
    are used to eradicate C.difficile, the
    antibiotics kill many of the good
    gastrointestinal flora species, but only kill
    non-spore forms of C.difficile. The C.difficile
    spores are still present. In order to eliminate
    C.difficile spores you need to have some good
    normal flora present. How can I re-establish my
    gastrointestinal flora to eliminate
    C.difficile?Numerous of studies have proven
    certain probiotics to be a successful solution
    for recurrent C.difficile diarrhea.

Saccharomyces boulardii
  • Saccharomyces boulardii
  • A non-disease causing yeast that survives passage
    through the intestinal tract, unaffected by
    antibiotics (although it will be killed by
    antifungal drugs).
  • Based on preliminary data indicating that this
    yeast could prevent antibiotic-associated
  • Numerous studies, have gone on to prove
    Saccharomyces boulardii, as an effective
    treatment for active C.difficile diarrhea.
  • Saccharomyces boulardii disrupts toxins that
    would normally irritate the intestines,
  • In addition, Saccharomyces boulardii stimulates
    the intestinal immune system to secrete
    C.difficile toxin A-specific antibodies (IgA) in
    the gut (Qamar et al., 2001).
  • It is then eliminated after supplementation is

Allergy Research Group
  • http//

Thorne Lactobacillus sporogenes
  • Lactobacillus sporogenes represents a
    breakthrough in Lactobacillus supplementation
  • a safe, stable, effective, L()-lactic-acid-produc
    ing organism.
  • Lactobacillus sporogenes binds cholesterol in the
    gut, and may inhibit the cholesterol-producing
    enzyme HMG CoA Reductase.
  • It requires no refrigeration or special

  • http//
  • http//
  • Manufacture sites
  • http//
  • http//
  • http//
  • Microbial Ecology and Dysbiosis in Human
    Medicine Excellent Article