S.R. Eddy (2001) Noncoding RNA genes and the modern RNA world, Nature Reviews Genetics, 2:919-929

1
Transcription in Eukaryotes
Three major classes of RNA
Type Encodes Copies/genome Total
RNA mRNA proteins Single or few
10 tRNA functional RNA Few
20 rRNA functional RNA Many 70
But there are more!!!
S.R. Eddy (2001) Noncoding RNA genes and the
modern RNA world, Nature Reviews Genetics,
2919-929
2
Epitope Tagging of RNA Polymerase II
Genetically tag Polymerase II subunit Grow
cells in radioactively labeled amino acids Allow
assembly of active polymerase Add antibody that
binds epitope Grab antibody and what ever it
is bound to SDS electrophoresis
3
Transcription
RNA polymerase II
4
Transcription
RNA polymerase II
5
Transcription in Eukaryotes
Class II promoters
Core Promoter TATA box TFIIB Recognition
Element (BRE) Initiator (Inr) Downstream
Promoter Element (DPE) Upstream Promoter Element
6
Transcription Initiation in Eukaryotes
General Transcription Factors and the
Pre-initiation complex
7
Transcription Initiation in Eukaryotes
TBP binding
8
Transcription Initiation in Eukaryotes
9
Transcription Initiation in Eukaryotes
10
Schematic Model
Elongation of Transcription in Eukaryotes