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Properties of Water

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pH and pKs exists in Amino Acids. NH2 CHR COOH. NH3 CHR COO. Zwitterion. Buffers- organic acid and its conjugate base. CH3COOH. CH3COO Na ... – PowerPoint PPT presentation

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Title: Properties of Water


1

Properties of Water

2

Noncovalent Bonding Ionic interaction Hydrogen
Bonds van der Waals Forces Thermal Properties of
Water Solvent Properties of Water Hydrophilic,
hydrophobic, and amphipathic molecules Osmotic
pressure

3

Ionization of Water Acids, bases, and
pH Buffers Physiological buffers

4

Water is Polar

-

5

Hydrogen Bonding
I I I I I I I

I I I I I I I
6

NaCL
Na Cl-

Na
Cl-
7

Hydrophobic Bonding

8

9

Osmosis

10

Isotonic

Hypotonic
Hypertonic
11

Isotonic

Hypotonic
Hypertonic
12

pH

13

Ionization of Water


-
2H2O    H3O OH
14

OH-
H30

15

pH - log ( H3O ) pOH - log ( OH- )

at 25 degrees C Kw ( H3O ) (OH- ) 1 X 10
-14th M
pH pOH 14 so the pH scale is 0-14
16

Acids and Bases

17

Equilibrium
Base
Acid

18

Acid
Base ?

Acid
Equilibrium ?
Acid
19

Phosphoric Acid H3PO4

pK1 2.1 pK2 6.7 pK3 12.3
20

The Biological Importence of

pH and pKs exists in Amino Acids
NH2 CHR COOH
NH3 CHR COO
Zwitterion
21

Buffers- organic acid and its conjugate base

CH3COOH CH3COO Na
1) Buffer curves have similar shapes and only
shift along the pH axis 2) The pH at each
midpoint is equal to the PKa, also called the pI
3) The slope near the pKa is lower and means that
the buffering capacity is best
22

Buffering Capacity Buffer Strength

O.05 M vs 0.10 M Buffers
23

Physiological Buffers

CO2 / H2CO3

CO2 H2O H HCO3
pKa 6.37
Blood and Interstitial Fluid
24

Physiological Buffers
2

H2PO4 OH HPO4
H2O
pKa 7.2
Intracellular Buffer
25

Physiological Buffers

Amino Acids and Proteins
Zwitterions - Amino Acids can act as an acid or
base
NH3 CHR COO
pKa ca. 7
Proteins - Serum Albumin
26
End Water, pH and Buffers
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