Diapositivo 1

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Biologia Sintética um modelo para produção de
hidrogénio Paula Tamagnini

• Microbiologia Celular e Aplicada
• Pedro Moradas-Ferreira
• Bioremediação e bioprodutos
• Stress oxidativo (bactérias leveduras)

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BioModularH2 (FP6, NEST-2005-Path-SYN, Contract
043340) Engineered Modular Bacterial
Photoproduction of Hydrogen
Members of the consortium Instituto de Biologia
Molecular e Celular (Portugal) École
Polytechnique (France) Universidad Politécnica
de Valencia (Spain) Uppsala Universitet
(Sweden) University of Sheffield (UK) Weizmann
Institute of Science (Israel)
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Standard parts and modules that will be
introduced into complex systems
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Parts
e. g. physiological conditions nutrients
levels, etc.
MIT database of biological parts and devices
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e.g. BioModularH2 devices
high O2 pressure
low O2 pressure
Oxygen sensor device
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Photoautotrophic chassis
Synechocystis sp. PCC 6803

• unicellular non-N2-fixing cyanobacterium
• naturally transformable
• well studied
• vectors for gene transfer available

Synechocystis sp PCC 6803 Chromosome 1 GC
Content 47 Phenotype Photosynthetic, Ocean
carbon cycle, Motile,
Photoautotroph, Facultative Size 3573 Kb, 3168
orfs
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Biomodular H2
In silico analysis Computational design of
parts Computational design of devices
Synthesis of parts Assembly of devices Preparation
of the chassis
Characterization of parts and devices Introduction
into the chassis Evaluation of the final product
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Final goal
Our custom cyanobacterium chassis together with
the designed devices will harvest solar energy
for H2 production.