Genetic variation of the Bloom-Forming Cyanobacterium Microcystis aeruginosa within and among Lakes: Implications for Harmful Algal Blooms

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Genetic variation of the Bloom-Forming
Cyanobacterium Microcystis aeruginosa within and
among LakesImplications for Harmful Algal Blooms

• APPLIED AND ENVIRONMENTAL MICROBIOLOGY
• Oct. 2005, p. 6126-6133
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• ????????- Microcystis aeruginosa
• ?? ???????
• ????
• ????(???????????)
• ????????
• ???????????

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???? (??????)
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(BG-11, n3)
(WC, n2)
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G-test

• G-test, although it is also sometimes called a
  log-likelihood test or a likelihood ratio test.
• With sample sizes between 50 and 1000, it
  generally doesn't make much difference which test
  you use, so you shouldn't criticize someone for
  using the chi-square or G-test
• The G-test of independence is used for two
  nominal variables, not one.

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• Survival rate
• Range 0-30
• Mean SD , 7.4 2.1
• Relationship
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• ??????
• (G 38.7, df4, plt0.001)

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• PCR result of HIP primers

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DNA???????

• ?? ???Seq 1 AATTAATTCG
  ?ATTSeq 2 AACCAATTCG ?ACCSeq 3 AGTTAATTCG
  ?GTT
• Seq 1?Seq 2???????,Seq 1?Seq 3???????????????Wrigh
  ts F???????????alleles?

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AMOVA
10 colonies ? 3 stranis

• Microcystis aeruginosa
• Population
• Colony
• Strain

8 colonies ? 8 stranis
7 colonies ? 1 stranis
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• http//www.docstoc.com/docs/2370106/Analysis-of-Mo
  lecular-Variance-(AMOVA)

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???????

• ???()
• Percent distinct genotypes
• Range 42-100
• Mean SD , 78 7.8

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• AMOVA
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• p value of Fsc

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• AMOVA
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• 41???????????????
• 59???????????????

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1. ?(p value of Fsc)????????
2. ???

Paper Course Note
G-test ?2 ???,??????
AMOVA ANOVA ????????
F-test (or F-statistics) F-statistics ?????
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• Q A section

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Phi coefficient
the phi coefficient f or rf is a measure of
association for two binary variables.