R.A.P.I.D.

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R.A.P.I.D. LT Food Security System
Dennis DAlfonso National Sales Manager
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Idaho Technology (ITI)

• Company Profile
• Idaho Falls, ID (1990-1999)
• Salt Lake City, UT (1999-PRESENT)
• 200 Employees
• Hardware, Firmware, Software
• RD Molecular Biology, Chemistry
• IT Biochemistry and Freeze Drying
• Production Assembly
• Sales, Marketing, and Customer Service
• 3 Facilities Salt Lake City
• Research and Development
• Software Engineering
• Chemistry Production

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Proven Technology IN and OUT of Lab
R.A.P.I.D. System is a rugged, portable BW
detection system for the field and Lab. Freeze
dried reagents for Pathogen detection.
1991
RapidCycler
1996
LightCycler
RAZOR System is a rugged, portable and battery
powered BW detection system for the front line
soldier and first responder.
1999
R.A.P.I.D. Reagents
RAZOR
2002
The R.A.P.I.D. LT is a real-time PCR instrument
capable of analyzing samples for the presence of
targeted nucleic acid sequences.
R.A.P.I.D. LT
2006
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ITI Customers

• US Government
• DoD
• DHS
• USDA
• EPA
• US Commercial
• Fortune 200 companies
• International
• Europe
• Italian Government
• Czech Republic
• UK
• Switzerland
• Finland
• Australasia
• Australia
• Singapore
• Japan

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R.A.P.I.D. LT Food Security System
Benefits

• Sensitive
• Specific
• Very fast PCR (in less than an hour!)
• Designed for minimally trained users
• Freeze-dried reagents
• Simple set-up
• Point and click automated software
• Pooling enabled
• Expands testing by 5X
• Reduces cost in a negative world
• Simultaneous testing of all targets

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Daily Capacity
55 minute run Single Unit Capacity 8 hr day
256 reactions 16 hr 512 reactions 24 hr 768
reactions Pooling Capacity 8 hr day 1280
reactions 16 hr day 2560 reactions 24 hr day
3840 reactions
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R.A.P.I.D. LT Workflow
Continues to Step 3 4 gtgt
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R.A.P.I.D. LT Workflow
Continues to Step 5 6 gtgt
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R.A.P.I.D. LT Workflow
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Enrichment and Preparation

• Enrichment Times

Target Time
Salmonella 16 hours
E.coli O157H7 8 hours
Listeria spp. 24 hours
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Freeze Dried Reagents

• Freeze-Dried Reagents contain all components in a
  ready-to-use product
• Contains all reaction components
• Room temperature storage
• Reduces set-up time and minimizes operator error
• Cost savings in time and expertise

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R.A.P.I.D. LT Software Features

• Easy-to-use step-by-step Wizard User Interface

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Real-Time Fluorescent PCR

• Temperature cycling
• Fluorimeter acquisition
• Real time read out

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Automated Data Interpretation

• Positives results indicated by Warning dialog box
• Verify by following appropriate BAM or FSIS
  guidelines

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Idaho Technology Assays

• Food Pathogen Assays available (Bio-Defense RAPID
  RAZOR)
• Salmonella
• E. coli O157
• Listeria monocytogenes
• Campylobacter
• Food Security System AOAC Approval Timeline
• March 2008 Salmonella (AOAC-RI Approval 030803)
• January 209 Listeria spp. (AOAC-RI Approval
  010901)
• Spring 2009 E. Coli O157H7
• Other Assays Available
• -Anthrax -Avian Influenza
• -Plague -Tularemia
• -Botulism -FMDV
• -Ricin -Cryptospridium
• -Smallpox -Brucella

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Training and Customer Support

• Training programs
• Initial training included with system purchase
• Most lab techs trained in ½ day
• 24/7 technical support available
• RAPID LT includes 1 year warranty

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In Summary
The R.A.P.I.D. LT FSS offers

• Complete product solution hardware, chemistry,
  software and technical support
• Accuracy and specificity
• Ease of use
• Extensive scientific support and expertise
• Shortened enrichment times faster total time to
  result
• Pooling capability, cost efficiency
• Only true walk-away platform on the market
• Freeze-dried reagents/no proprietary media
• Ability to run other commercially available
  products

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Follow up Contact

• Idaho Technology, Inc.
• 390 Wakara Way
• Salt Lake City, Utah 84108, USA
• 801-736-6354 Main Phone Number
• www.idahotech.com
• Dennis DAlfonso
• National Sales Manager
• 801-230-8614 Cellular Phone Number
• dennis_dalfonso_at_idahotech.com