Sarcofluor

1
Sarcofluor

• P.C. Duarte, B.M. Daft, P.A. Conrad, A.E.
  Packham, I.A. Gardner
• Department of Medicine and Epidemiology,
  Department of Pathology, Microbiology
  Immunology, California Animal Health Food
  Safety Laboratory System, School of Veterinary
  Medicine, University of California

2
Sarcofluor

• Quantitative IFAT test
• Merozoites of UCD-1 Sarcocystis neurona isolate
  (Marsh et al., 1998)
• Serum (110) and/or CSF (15)
• Using 48 gold-standard infected non-infected
  horses Sensitivity 88.9, specificity 100
  (Duarte et al., 2003)
• Field validation using 110 gold-standards Serum
  (80) sensitivity 83.3, specificity 96.9
  CSF ( 5) sensitivity 100, specificity
  99 Titer-specific LRs 0.03-187 for
  titers lt10-640 (Duarte et al., 2004)

3
Sarcofluor

• Titer-specific probabilities of EPM

CSF titer Probability of EPM
lt 5 5 lt 1 92
Serum titer Probability of EPM
10 20 40 80 160 320 640 7 16 33 55 76 89 95
4
Sarcofluor

• Cross-reactivity to other equine Sarcocystis
  species Experimental S. fayeri infection
  (Saville et al., 2004)
• CSF blood contamination (Finno et al., 2006)