The%20Mechanism%20of%20Protein%20Splicing: - PowerPoint PPT Presentation

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The%20Mechanism%20of%20Protein%20Splicing:

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Title: The%20Mechanism%20of%20Protein%20Splicing:


1
The Mechanism of Protein Splicing the
movie Animation by Maurice W. Southworth
As seen in InBase, Francine Perler, Curator
2
(No Transcript)
3
SH
SH
O
CH2
CH2
C
C
NH
NH
N-extein
Cys
Asn
Cys
O
Intein
C-extein
4
SH
SH
O
CH2
CH2
C
C
NH
NH
N-extein
Cys
Asn
Cys
O
Intein
C-extein
5
O
SH
SH
C
CH2
CH2
N-extein
C
NH
NH
Cys
Asn
Cys
O
Intein
C-extein
6
O
SH
C
S
CH2
N-extein
CH2
C
NH
NH2
Cys
Asn
Cys
O
Intein
C-extein
7
O
C
SH
S
N-extein
CH2
CH2
C
NH
NH2
Asn
Cys
Cys
O
Intein
C-extein
8
O
SH
C
CH2
N-extein
S
CH2
NH
C-extein
C
NH2
Asn
Cys
O
Intein
9
SH
O
CH2
C
N-extein
S
C-extein
CH2
NH
C
NH2
Asn
Cys
O
Intein
10
O
SH
C
CH2
N-extein
S
C-extein
CH2
NH
C
NH2
Asn
Cys
O
Intein
11
O
SH
CH2
C
N-extein
S
C-extein
CH2
NH
C
NH2
Cys
Asn
O
Intein
12
SH
O
CH2
C
N-extein
S
C-extein
NH
CH2
C
NH2
Cys
Asn
O
Intein
13
O
SH
CH2
C
N-extein
S
C-extein
NH
CH2
C
NH2
Cys
Asn
O
Intein
14
O
S
C
CH2
N-extein
SH
C-extein
NH
CH2
C
NH2
Cys
Asn
O
Intein
15
O
S
C
CH2
N-extein
SH
C-extein
CH2
NH
C
NH2
Cys
Asn
O
Intein
16
O
S
C
CH2
N-extein
SH
C-extein
NH
CH2
C
NH2
Cys
Asn
O
Intein
17
O
C
S
N-extein
CH2
SH
C-extein
CH2
NH
C
NH2
Cys
Asn
O
Intein
18
O
C
N-extein
S
CH2
SH
C-extein
CH2
NH
C
NH2
Cys
Asn
O
Intein
19
(No Transcript)
20
(No Transcript)
21
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22
O
C
N-extein
S
CH2
NH2
SH
C-extein
C
O
NH
CH2
CH2
C
NH2
O
Cys
Asn
Intein
23
O
C
N-extein
S
CH2
NH2
SH
C-extein
C
O
NH
CH2
CH2
C
NH2
O
Cys
Asn
Intein
24
O
C
N-extein
S
CH2
NH2
SH
C-extein
C
O
NH
CH2
CH2
C
NH2
O
Cys
Asn
Intein
25
O
C
N-extein
S
CH2
NH2
O
SH
C-extein
C
NH
CH2
CH2
C
NH2
O
Cys
Asn
Intein
26
O
C
N-extein
S
CH2
NH2
O
SH
C-extein
C
NH
CH2
CH2
C
NH2
O
Cys
Asn
Intein
27
O
C
N-extein
S
CH2
O
NH2
SH
C-extein
C
NH
CH2
CH2
C
NH2
O
Cys
Asn
Intein
28
O
C
N-extein
S
CH2
O
C-extein
SH
NH2
C
NH
CH2
CH2
C
NH2
O
Cys
Asn
Intein
29
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
O
Cys
Asn
Intein
30
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
31
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
32
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
33
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
34
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
35
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
36
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
37
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
38
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
39
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
40
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
41
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
42
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
43
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH
NH2
CH2
CH2
C
NH2
O
Cys
Asn
Intein
44
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
45
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
46
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
47
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
48
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
49
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
50
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
51
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
52
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
53
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
54
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
55
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
56
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
57
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
58
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
59
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
60
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
61
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
62
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
63
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
64
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
65
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
66
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
67
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
68
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
69
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
70
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
71
O
C
N-extein
S
CH2
O
C-extein
SH
C
NH2
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
72
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
73
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
74
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
75
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
76
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
77
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
78
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
79
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
80
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
81
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
82
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
83
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
84
O
C
N-extein
S
CH2
NH2
O
Cys
C-extein
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
85
O
C
S
N-extein
CH2
NH2
O
C-extein
Cys
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
86
O
C
S
N-extein
CH2
NH2
O
C-extein
Cys
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
87
O
C
S
N-extein
CH2
NH2
O
C-extein
Cys
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
88
O
C
S
N-extein
CH2
NH2
O
C-extein
Cys
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
89
O
SH
C
N-extein
CH2
NH
O
C-extein
Cys
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
90
O
SH
C
N-extein
CH2
NH
O
C-extein
Cys
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
91
SH
O
CH2
C
N-extein
NH
O
C-extein
Cys
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
92
SH
O
CH2
C
N-extein
NH
O
C-extein
Cys
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
93
SH
O
CH2
C
N-extein
NH
O
C-extein
Cys
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
94
SH
O
CH2
C
NH
N-extein
Cys
C-extein
O
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
95
SH
O
CH2
C
NH
N-extein
Cys
C-extein
O
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
96
SH
O
CH2
C
NH
N-extein
Cys
C-extein
O
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
97
SH
O
CH2
C
NH
N-extein
Cys
C-extein
O
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
98
SH
O
CH2
C
NH
N-extein
Cys
C-extein
O
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
99
SH
O
CH2
C
NH
N-extein
Cys
C-extein
O
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
100
SH
O
CH2
C
NH
N-extein
Cys
C-extein
O
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
101
SH
O
CH2
C
NH
N-extein
Cys
C-extein
O
SH
C
NH
CH2
CH2
C
NH2
Cys
Asn
O
Intein
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