Using a restriction enzyme and DNA ligase to make recombinant DNA

1
Using a restriction enzyme and DNA ligase to make
recombinant DNA
Restriction site
5?
3?
G A A T T C
DNA
3?
5?
C T T A A G
Restriction enzyme cutsthe sugar-phosphatebackbo
nes at each arrow
G
A A T T C
C T T A A
G
Sticky end
A A T T C
G
G
DNA fragment from another source is added. Base
pairing of sticky ends produces various
combinations.
C T T A A
Fragment from differentDNA molecule cut by
thesame restriction enzyme
G
G
A A T T
C
A A T T C
T T A A
C T T A A
C
G
G
One possible combination
DNA ligaseseals the strands.
Recombinant DNA molecule
2
Using the Ti plasmid to produce transgenic plants