Informationsverarbeitung%20in%20Bakterien - PowerPoint PPT Presentation

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Informationsverarbeitung%20in%20Bakterien

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Assay of LacZ activities on. plates and in liquid media. Promoter ... ori colE1. IR. tnp. BcuI. ldh. MPN673 -10 -35 -35 -10 81 -160 81 -118 81 -84 81 -53 ... – PowerPoint PPT presentation

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Title: Informationsverarbeitung%20in%20Bakterien


1
Promoter Analyses in Mycoplasma pneumoniae
Cloning of potential promoter fragments Integrati
on of the lacZ-fusion into the chromosome
by transposition Assay of LacZ activities on
plates and in liquid media
2
Analysis of the ldh Promoter
HindIII
HindIII
ldh
-10
-35
-35
-10
MPN673
81
-160
81
-118
81
-84
81
-53
1
-85
-160
81
The lacZ fusion reflects promoter activity
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