Steinernema ashiunense sp' n', a new entomopathogenic nematode in the genus Steinernema Travassos, 1

1
Steinernema ashiunense sp. n., a new
entomopathogenic nematode in the genus
Steinernema Travassos, 1927 from Japan

• Long K. Phan, Shuhei Takemoto Kazuyoshi Futai

2
Life cycle of entomopathogenic nematodes
3
Identification methods
Morphology and morphometric light microscopy and
SEM
Molecular ITS sequences
Software Clustal X 1.64 and Paup 4.0
4
feltiae-kraussei-oregonense group IJ length
600-1000 µm Lateral line 5-8 lines
glaseri group IJ length gt900 µm Lateral
field 9 lines
carpocapsae group IJ length lt 500 µm
bicornutum group IJ with two horn like
structure in the head
afine group Lateral field 4 lines
5
IJ body length 768 (720-800) µm
6
Phylogenetic relationships within the
feltiae-kraussei-oregonense group
A Single unrooted Maximum Parsimony tree B
Single rooted Maximum Parsimony tree
7
Diagnosis and relationships with other species
The lateral field of this new species has five
equally developed ridges that can be
distinguished from all species in the
feltiae-kraussei-oregonense
Steinernema kraussei and S. oregonense have five
equally developed ridges and two weak
submarginal ones (total 7 ridges)
Steinernema feltiae, S. robustispiculum and S.
sangi are characterised by 8 ridges but two sub
marginal and two central ridges less distinct
Steinernema akhursti and S. jollieti have 6
equally developed ridges,
S. litorale, S. silvaticum and S. weiseri have 8
equally developed ridges.
8
Multiple sequence alignment of the ITS1 5.8S
ITS2 of Steinernema ashiunense sp. n. with two
closely related species, S. robustispiculum and
S. monticulum
9
The new species differs from two closely species
by 9 unique substitutions in the ITS1 8
unique substitutions in the ITS2
10
Thank you