Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum)

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Effects of 2,4-D on Anthocyanin Pigments in Red
Norland Potatoes (Solanum tuberosum)

• Shannon Engelman 2007

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University of MNDepartment of Horticulture
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2,4-dichlorophenoxyacetic acid (2,4-D)
http//files.agrichem.net/agrbv/artinfo/foto.org/2
,4,20d20Amine20a.JPG
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(No Transcript)
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Background

• Light intensity and hue angle decreases (Rosen,
  et al.)

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Components of Color

• Light intensity
• Chroma (saturation)
• Hue angle

www.xrite.com/documents/literature/en/L11-029_colo
r_guide_en.pdf
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Components of Color

• Light intensity
• Chroma (saturation)
• Hue angle

www.xrite.com/documents/literature/en/L11-029_colo
r_guide_en.pdf
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Components of Color

• Light intensity
• Chroma (saturation)
• Hue angle

www.xrite.com/documents/literature/en/L11-029_colo
r_guide_en.pdf
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Components of Color

• Light intensity
• Chroma (saturation)
• Hue angle

www.xrite.com/documents/literature/en/L11-029_colo
r_guide_en.pdf
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Background

• Light intensity and hue angle decreases (Rosen,
  et al.)
• Coloration affects sales (Fults, et al.)

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Background

• Light intensity and hue angle decreases (Rosen,
  et al.)
• Coloration affects sales (Fults, et al.)
• 2,4-D harmful, LD50 of 1500 ppm (MSDS found at
  http//fscimage.fishersci.com/msds/96919.htm)

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Goal

• To determine visual color, anthocyanin
  accumulation, and UFGT accumulation differences

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Anthocyanin Biosynthetic Pathway

• LDOX UFGT
• leucocyanidin?anthocyanidin?anthocyanin
  3-glucoside
• (Keifenheim, et al.)

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Hypotheses

• With 2,4-D application
• Increase in UFGT accumulation

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Hypotheses

• With 2,4-D application
• Increase in UFGT accumulation
• Change in color (Rosen, et al.)

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Hypotheses

• With 2,4-D application
• Increase in UFGT accumulation
• Change in color (Rosen, et al.)
• Increase in anthocyanin accumulation

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Methods
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Colorimetry
http//www.recreonics.com/images3/pocket_colorimet
er.gif
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Components of Color

• Light intensity
• Chroma (saturation)
• Hue angle

www.xrite.com/documents/literature/en/L11-029_colo
r_guide_en.pdf
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Anthocyanin Accumulation
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Quantifying UFGT

RNA
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Quantifying UFGT

cDNA
RNA
reverse transcription
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Quantifying UFGT

Amplified UFGT
cDNA
RNA
reverse transcription
qPCR
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Results
S
S
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Light Intensity, Chroma, Hue Angle, Anthocyanin
Accumulation
Field Study
Greenhouse Study
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Light Intensity, Chroma, Hue Angle, Anthocyanin
Accumulation
Field Study
Greenhouse Study
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Light Intensity, Chroma, Hue Angle, Anthocyanin
Accumulation
Field Study
Greenhouse Study
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Light Intensity, Chroma, Hue Angle, Anthocyanin
Accumulation
Field Study
Greenhouse Study
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Light Intensity, Chroma, Hue Angle, Anthocyanin
Accumulation
Field Study
Greenhouse Study
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UFGT Field Data
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UFGT Greenhouse Data
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Conclusion

• Visual color significantly changed (light
  intensity, hue angle)

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Conclusion

• Visual color significantly changed (light
  intensity, hue angle)
• Anthocyanin accumulation unchanged

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Conclusion

• Visual color significantly changed (light
  intensity, hue angle)
• Anthocyanin accumulation unchanged
• UFGT accumulation decreased

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Future Work

• Look at petunidin (purple) vs. pelargonidin (red)
  anthocyanins

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Acknowledgements

• Dr. Cindy Tong
• Harpartap Mann
• Dr. Carl Rosen
• Ms. Fruen
• Team Research

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Effects of 2,4-D on Anthocyanin Pigments in Red
Norland Potatoes (Solanum tuberosum)

• Shannon Engelman 2007