Measuring Alcohol and Stress Interactions with Structural and Perfusion MRI

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Measuring Alcohol and Stress Interactions with
Structural and Perfusion MRI

• Chris Wyatt
• External Collaboration

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Introduction

• Our goal is to use MRI to examine the effects of
  chronic alcohol self-administration on brain
  structure and function in a group of monkeys that
  have been mother-reared or nursery-reared.
• Team
• Jim Daunais (WFUHS PI)
• Bob Kraft (WFUHS MR Physicist)
• Chris Wyatt (VT Engineering)
• Vidya Rajagopalan (VT Engineering)
• Killian Pohl (NA-MIC Core 1)

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12.3
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Size comparison of human, rhesus, and cynomolgus
brain.
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NA-MIC Related Goal

• Implement and validate algorithms for
• brain extraction
• white-gray matter segmentation
• subcortical structure segmentation

Size comparison of human, rhesus, and cynomolgus
brain.
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Vervet model

• The animals for this project were divided into
  multiple serial cohorts from a separate project.
• That project switched from a rhesus to a vervet
  animal model.
• So we started over with a vervet model in March.

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Methods Atlas Formation
Pass 1 based on 1
Pass 2 Slicer 3 EMSegment
Initial Tissue Atlas
Final Tissue Atlas
Manual Subcortical Delineation
Final Atlas
Training Subject Images
1 M. Styner et al. Automatic brain segmentation
in rhesus monkeys. Proc SPIE Medical Imaging
Conference, Proc SPIE Vol 6512 Medical Imaging
2007.
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Methods Segmentation
Brain Extraction
Deformable Registration of Priors
EMSegment in Slicer 3
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Products

• Vervet template, tissue, and subcortical atlas
• Tutorial Using Slicer3 to segment vervet images

http//www.bsl.ece.vt.edu/data/vervet_atlas/vervet
.php
http//www.bsl.ece.vt.edu/tutorials.php
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Future

• Acquisition of baseline vervet images for atlas
  development
• Vervet atlas completed
• Training and Induction 3-4 months
• ad-lib access for 12 months
• Final structural scans August 2009