Comparative proteomics of HIV1 latently infected and uninfected cells: upregulation of antiapoptotic

1
Comparative proteomics of HIV-1 latently infected
and uninfected cells upregulation of
anti-apoptotic proteins as a marker for latency
Objective Identification of surface markers by
performing comparative proteomics of established
cell models of latently HIV-1 infected cells with
parental cell lines.
Method
A)
B)
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1 2 3 4
2
Surface proteins differentially expressed in CEM
and ACH2
3
Confirmation of MALDI results
B)
A)
BTK-GFP
4
Infected cells are more dependent on
anti-apoptotic proteins then uninfected cells
5
Infected cells are more sensitive to drugs
targeting anti-apoptotic proteins
6

• Conclusion
• Using a biotin-directed affinity purification
  method, we identified membrane or membrane
  associated proteins that can be divided into two
  categories cell-adhesion/structure, and cell
  survival.
• The survival protein BTK and the anti-apoptotic
  proteins XIAP were found to be up-regulated in
  HIV latently infected cells, suggesting that
  infected cells were more dependent on
  anti-apoptotic mechanisms to maintain their
  survival.
• Using drugs that decrease the expression of
  anti-apoptotic proteins, we showed that HIV-1
  latently infected cells were more sensitive to
  drug treatment than uninfected cells.

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