Micropipettes

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Micropipettes

• Measuring volumes which are what we scientists
  call
• very, very small

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MICROPIPETTE RULES

• Dont set the pipettes past their intended range
  (high or low), even though you can.
• ALWAYS return the dial to the highest setting
  before putting the pipette away.
• ALWAYS return the pipette to its correct storage
  bin

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High Range 100-1000 µL Use large blue or white
tips Digits from top to bottom represent
1000s 100s 10s The 1s are indicated by the
tiny white tick marks in the dial with the 10s.
Marks are in increments of 2 µL This pipette is
set to 1000 µL
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This pipette is set to 998 µL
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This pipette is set to 990 µL
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Mid-range 5.0-50.0 µL 10.0-100.0 µL,
20.0-200.0 µL Range is printed on the button or
on the side Use medium sized yellow or white tips
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10.0-100.0 µL This pipette is set to its maximum
100.0 µL Digits represent 100s 10s 1s Tick
marks represent 0.1s in increments of 0.2 µL
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Low Range 0.50-10.00 µL 2.00-20.00 µL Digits
represent 10s 1s 0.1s Tick marks are 0.01s
in increments of 0.02 µL Use small white tips
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Rainin 0.50-10.00 µL pipettes use a unique
tip Clear tip has an extra wide mouth that
surrounds and goes over the barrel and eject
bar. These tips can ONLY be used with the Rainin
0.5-10
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• Feel the stops before you begin taking up or
  dispensing any liquid.

1st stop Exact volume to which the pipette is
set. Press to this stop before immersing pipette
tip in the desired solution. 2nd stop Use when
dispensing solution. Pushes an extra volume of
air to dislodge any solution adhering to the tip.
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How to pipette
Great summary
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• Extract and dispense SLOWLY.
• When dispensing, touch the tip
• to the side or bottom of the tube,
• or if there is liquid already in the
• tube, touch the tip to the liquid.
• When taking up or dispensing
• liquid, dont put the tip any deeper
• into the liquid than necessary.

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• NEVER hold pipette so that the tip is higher than
  the handle or liquid leaks into the barrel (BAD!)
• Always hold pipettes vertically
• when in use.
• Check to be sure the tip is on securely.
• Only open the tip box when getting a tip DONT
  LEAVE IT OPEN!