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Weaning mice

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Weaning mice Weaning mice Remove weaning-age mice (usually 21 days) from the parent cage. Separate the pups by sex. Mark the parent cage with a separation card; mark ... – PowerPoint PPT presentation

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Title: Weaning mice


1
Weaning mice
2
Weaning mice
  • Remove weaning-age mice (usually 21 days) from
    the parent cage.
  • Separate the pups by sex.
  • Mark the parent cage with a separation card mark
    the weaned cages with separation stickers

3
Before you start
  • Check the problem log to see if you expect to
    have any separations
  • Check the room for green OC cards

4
Before you start
  • Check your watch!! What time is it?
  • If you dont have a watch, check the time on your
    pager

5
Why are you checking the time?
  • So you can fill out the Special Services Log when
    you are done.

Date Principal Investigators Name Types of Service Cage Separation ( of cages) Types of Service Other Services Number of Hours Performed By (Print Name) Initials



6
Before you start
  • Double-check the OC card
  • Is it a supervisor check?
  • Double-check that the weaning due date is
    correct- look at the weaning date calendar.

7
Weaning mice
  • Remove weaning-age mice (usually 21 days) from
    the parent cage.
  • Separate the pups by sex.
  • Complete the necessary paperwork

8
Can you tell sex by color?
9
Can you tell sex from how theyre dressed?
10
We determine the sex of mice by examining the
genitals
11
It isnt so easy to sex pups
Female
Male
Female
  • For pups, the ano-genital distance is used to
    determine sex.

12
The ano-genital distance is longer in males than
in females
13
  • Female pups have a shorter anogenital distance
  • Female pups have nipples (which may not be
    visible if the pups are furry)

14
What sex is this pup?
l
l
Male Female
15
Sex pups 2 at a time
  • If they look the same, try again until you get
    one that looks different.
  • The one with the longer anogenital distance is
    the male. The other is the female.
  • Keep one pup of known sex, and use it to compare
    against the rest of the pups.

16
What sex is this pup?
Female Male
17
What sex is this pup?
Female Male
18
Male Female
19
Male Female
20
Female Male
l
l
21
Weaning set-up
  • Parent cage
  • Male pup cage
  • Female pup cage
  • Even if you have more than 10 pups, start by
    separating the pups by sex into two cages.

22
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23
Put some food on the floor for the pups
24
Housing pups
  • 5 females per cage
  • 4 males per cage
  • Recheck the sex
  • Put pellets of food on the cage floor
  • Complete the green separation cards and stickers
  • House the separated cages on the separation shelf

25
Separation cards
  • The white sticker (with the separation number)
    goes on the green card.
  • Write the date and the number of new cages
    created on the white sticker

26
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27
  • Write the separation number on
  • The white cage card
  • Each of the green separation stickers

28
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29
Put the separated cages on the separation shelf
30
Before you finish
  • Update the census
  • Initial the addressed column in the problem log
  • Fill out the Special Services Log.

31
Update the census
  • Write the number of new cages created in the plus
    () column of the census.

32
Initial the addressed column in the problem log
33
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34
Fill out the Special Services Log.
  • Fill out the Log. Put the number of new cages
    created in the separation column.
  • Look at your watch to determine the time to
    complete the separation. The whole time
    (including paperwork) counts.

35
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The end
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