MnSOD from E. coli

1
Structure of Active site of MnSOD from E.
coli Free and bound to Azide (N3)
Gln 145 Trp 168
Tyr 34
OH Cu
N3
His 170
Thr 33 His 30
MnSOD from E. coli PDB structure 1VEW
Azide bound to MnSOD from E. coli PDB
structure 1MNG
The bound N3 anion suggests how O2 approaches
the active site.
2
Orientation of azide differs in structures of
Fe- and MnSOD from E. coli
N3
Azide bound to FeSOD from E. coli PDB
structure 1ISB
Azide bound to MnSOD from E. coli PDB
structure 1MNG
Azide chanes orientation in Fe structure, raises
question about active site flexibility.
3
The active site of FeSOD from M. tuberculosis is
very similar to that of MnSOD from E. coli
Gln 145
His 145
OH Cu
His 170
FeSOD from M. tuberculosis PDB structure 1IDS
MnSOD from E. coli
Similarity of structures suggests that inhibitors
of E coli MnSOD could be used against M.
tuberculosis.
4
Comparison of CuZnSOD active sties with and
without azide
Asn 139
Glu 133
Gly 139
Glu 133
Lys 136
Ser 140
Lys 136
Arg 141
Pro 62
Pro 62

• Azide complexed Bovine CuZnSOD Human
  CuZnSOD
• PDB 1SXZ PDB 1PU0