Chapter 14 Definitions of the Gene - PowerPoint PPT Presentation

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Chapter 14 Definitions of the Gene

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... homologous ssDNA to find each other TCA TC/T TGC TTC ACA ATG GTA/G CTC/T TTC/T CCA/G GAT TC S S C F T M V L F P D TC GAA TCC/T GGA/G AAA/G AGT/C ACC ATT ... – PowerPoint PPT presentation

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Title: Chapter 14 Definitions of the Gene


1
Almost all of molecular biology techniques
are based on this paper
2
Figure 1
  • Slow cooling was better
  • 2) More DNA more transformation
  • activity

3
Figure 2
DNA absorption at heated temp
DNA absorption after cooling
Activity correlates to change in absorption of
cooled DNA not warm DNA
4
Figure 3
5
Figure 4
Fast cooling?
1ug/ml to 20ug/ml
6
Figure 5
Salt dependent Annealing by Repressing the
repulsive Effect of phosphates By shielding them.
7
Figure 6
Non-homologous DNA makes it Harder for
homologous ssDNA to find each other
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TCA TC/T TGC TTC ACA ATG GTA/G CTC/T TTC/T CCA/G
GAT TC
S S C F T M V L F P D TC
GAA TCC/T GGA/G AAA/G AGT/C ACC ATT GTG AAG
CAA/G ATG
E S G K S T I V K Q M
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E S G K S T I V K Q M
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