??????????????F1-ATPase????? ??????????

1
??????????????F1-ATPase???????????????

• ?? ??
• ????????????????
• (??????????????)

2
?????????

• ??10nm(10??????????)
• ???????????????????????????????????
• ??????????????????
• ?????(??????????) ??? ??

3
??????????
??????1000km????????????
4
????????

• ATP?????F1-ATPase
• ???????????????

5
ATP????????????
????????? ????? ???(DNA,??????????)
????????ATP??
6
ATP???????
ATP??? (ADP?ATP)
???????????
7
ATP??????????
??
?
??
(F1-ATPase)
8
F1-ATPase????????
F1-ATPase
9
F1-ATPase???????!
(?????????????1.3µm)
Noji,H., Yasuda, R., Yoshida, M. Kinosita, K.
(1997) Nature
10
F1-ATPase???????!
(?????????????2µm)
11
1??ATP?120?????
Yasuda, R. et al. (1998) Cell
12
?????????????
120????????????????
?????????
ATP?????????????
13
?????????????
3µm
2µm
1µm
14
?????????????
3µm
2µm
1µm
15
?????????????
3µm
2µm
1µm
16
?????????????
120????????????????
?????????
ATP?????????????
100 !!

Yasuda, R. et al. (1998) Cell
17
??????
18
??????1???
?????????? ?????(??8000????) ?ATP??120?????
267????
Yasuda, R. et al. (2001) Nature
19
????????????
(1000????)
120???????90?????30?????
20
????????
ADP??
ATP??
ATP??
120?
ATP?? ??
90?
0?
ATP?? ??
Yasuda, R. et al. (2001) Nature
21
????????Ca2????????
????????

1. ??????????
2. ??????????
3. ??????????

????????
22
???????Ca2???????????
????
????? ???????
????
????
23
1???????Ca2????????????
24
????????Ca2????????
????????????
????
25
Ca2???????????
CaMKII
Ca2
??
26
Ca2??????????????????????
?????
Ca2?????????????
???
???
???????
????????????????