Preventative and Therapeutic treatment of resveratrol on Alzheimer

1
Preventative and Therapeutic treatment of
resveratrol on Alzheimers Disease using CL2006
and CL4176 C. elegan models

• Bradford Greaves

2
Need
http//www.searo.who.int/en/Section1174/Section119
9/Section1567/Section1823_8066.htm
3
Amyloid Beta Production
http//www.cnsspectrums.com/userdocs/articleimages
/62/Christensen_BigFig.jpg
4
Alzheimers Disease cont.
http//www.nia.nih.gov/NR/rdonlyres/A294D332-71A2-
4866-BDD7-A0DF216DAAA4/0/Alzheimers_Disease_Unrave
ling_the_Mystery.pdf
5
Oxidative Damage
http//www.nia.nih.gov/NR/rdonlyres/A294D332-71A2-
4866-BDD7-A0DF216DAAA4/0/Alzheimers_Disease_Unrave
ling_the_Mystery.pdf
6
The APP Processing
Resveratrol Promotes Clearance of Alzheimers
Disease Amyloid- Peptides, Marambaud 2005
Modified from Laurén et al., Nature (2009)
Senile Plaques
Figure 1
The ß amyloid peptide can act as a modular
aggregation domain, Link 2008
7
Soy isoflavone glycitein protects against beta
amyloid-induced toxicity and oxidative stress in
transgenic Caenorhabditis elegans,
Gutierrez-Zepeda 2005
Transgenic C. elegans as a Model in Alzheimers
Research, Wu 2005
Figure 2
8
Purpose

• To preventatively and therapeutically treat
  Alzheimers Disease with resveratrol using CL2006
  and CL4176 C. elegan models

9
Hypothesis

• Alternate Hypothesis Both preventive and
  therapeutic treatment of resveratrol on CL2006
  will lead to a decrease in amyloid beta
  production and for CL4176 there will be a delay
  of the onset of paralysis.
• Null Hypothesis There will be no change in
  amyloid beta production nor paralysis with the
  addition of resveratrol.

10
Resveratrols Coloration With Alzheimers Disease
http//www.chiro.org/nutrition/ABSTRACTS/Resveratr
ol_in_Prevention_and_Treatment.pdf
11
(No Transcript)
12
Vendor Cat Product Quantity Unit Total
Sigma-Aldrich Online Catalog R5010-500MG Resveratrol 1 281.50 281.50
Sigma-Aldrich Online Catalog S2188-1MG ?-Secretase inhibitor 1 267.50 267.50
Sigma-Aldrich Online Catalog P6148 Paraformaldehyde 1 28.00 28.00
Sigma-Aldrich Online Catalog P5368 PBS 1 42.60 42.60
Sigma-Aldrich Online Catalog M6250 2-Mercaptoethanol 1 23.00 23.00
Sigma-Aldrich Online Catalog T9284 Triton X-100 1 48.70 48.70
Sigma-Aldrich Online Catalog T0574 Trizma Buffer solution ? 1 25.90 25.90
Thiflavin S ?
Carolina 2009 173525 C. elegan culture kit 1 73.00 73.00
Carolina 2009 173500 N2 control 3 10.95 32.85
13
Do Ability

• CL2006 and CL4176 Staining and Paralysis
• Control food and Resveratrol available
• Fluorescent Microscope at school
• Prism not known (GFP)

14
Bibliography

• Bundschuh, K. "The worm in humans." 11 Nov.
  2008. Biotechnology and Life Sciences in
  Baden-Württemberg. 6 May 2009 lthttp//www.bio-pro.
  de/magazin/wissenschaft/archiv_2008/index.html?lan
  genartikelid/artikel/00452/index.htmlgt.
• Cheng, Louse. Compound in Red Wine May Fight
  Alzeimers. WebMD. 4 Nov. 2005. 15 Apr. 2009
  lthttp//www.webmd.com/alzheimers/news/20051104/com
  pound-in-red-wine-may-fight-alzheimergt.
• Duchen, Michael R. "Roles of Mitochondria in
  Health and Disease." Whereas some of the genes
  identified in the control of longevity appear to
  target mitochondria or at least to alter
  antioxidant defenses of the cell (Duchen, 2004.
  2004. Diabetes. 7 May 2009 lthttp//diabetes.diabet
  esjournals.org/cgi/content/full/53/suppl_1/S96gt.
• Halliwell, Barry, and John M.C. Gutteridge. Free
  Radicals in Biology and Medicine. 3rd ed. New
  York Oxford UP, 2004.
• Lagouge, Marie, Carmen Argmann, Zachary
  Gerhart-Hines, Hamid Meziane, Carles Lerin,
  Frederic Daussin, Nadia Messadeq, Jill Milne,
  Philip Lambert, Peter Elliott, Bernard Geny,
  Markku Laakso, Pere Puigserver, and Johan Auwerx.
  "Resveratrol Improves Mitochondrial Function and
  Protects Against Metabolic Disease by Activating
  SIRT1 and PGC-1a." Cell (2006). 20 May 2008.
• Lenker, David P. Professional Guide to Diseases.
  8th ed. New York Williams Wilkins., 2005
• Marambaud, Phillipe. Wine Compound May Help Cut
  Risk of Alzheimers Disease. 16 Nov. 2005. 24
  Apr. 2009 lthttp//www.sciencedaily.com/releases/20
  08/06/080617165716.htmgt.
• McKenzie, Maureen A. "Insulin Receptor-Like
  Protein." Patent Storm. 5 Oct. 1992. 18 Apr.
  2008.
• O,Neill, Bill. "In Methuselahs Mould." Feature
  2 (2004) 0014-017. PLoS Biology.
  lthttp//www.culturagalega.org/imaxes/docs/plos_lon
  xevidade.pdfgt.
• "Resveratrol Studies Suggest Broad Implications
  for SIRT1 Activation,in Neurodegenerative
  Diseases of Aging Such as Alzheimer's Disease (
  whether any of the Company's product ca...)."