Digesting macromolecules lab

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Digesting macromolecules lab
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Outline of the day

• Turn in your lab reports at the front
• More than 10 minutes late bad
• Any questions on last weeks lab?
• Quiz
• Introduction to the lab
• Lab!
• Check out
• Get a stamp
• Make sure I mark you down for attendance

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Quiz

• Ends 10 minutes after its started
• Ends at ____

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Lab this week!

• Exploring digestion!
• How carbohydrates, proteins, and fats are
  digested
• Effect of enzyme inhibitors on carbohydrate
  digestion
• Effect of pH on protein digestion
• Requirements for fat digestion

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Why do we eat?
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What are we doing when we eat?

• Digestion Dissolving food and breaking it down
  into smaller molecules
• Absorption The entry of molecules across the gut
  epithelium into the body

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What types of macromolecules are there?

• How do we digest them?

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Digesting food

• Dozens of enzymes help during digestion
• Enzymes make reactions go faster (and be more
  likely to occur)

Missing image Image showing enzymes reducing the
energy require for a reaction to progress.
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Today were going to see what effects the
digestion of macromolecules

• Using indicators to test for various steps of
  digestion
• E.g., Iodine changes color in the presence of
  starch
• Blue/black starch present
• Brown no starch present

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Detecting the breakdown of starch with iodine
Missing image IKI test samples
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Detecting the breakdown of starch by testing for
the presence of sugar
Missing image Benedicts test
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Detecting the breakdown of protein into amino
acids with egg whites
Missing image Egg white test
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Detecting the breakdown of fat into glycerol and
fatty acids
Missing text Description of lipid test
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Before you leave

• Clean up your work area
• Wash glassware and store upside down
• Show me your lab report so I can stamp it
• Need to have all data fields filled in
• Complete at home and then turn in at the
  beginning of next lab
• Remember that well have a quiz at the beginning
  of the next class
• 6-7 questions on todays lab
• 3-4 questions on the lab well do next week

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Notes for the instructor

• Add any relevant cleanup instructions to the
  final slide (that slide is a generic one Im
  adding to each presentation).
• The last few slides (on each biochemical test)
  were based on excellent slides from Kelli Elliott
  (of Orange Coast College) contact her for the
  images and other text that go along with them.
• Note also that the first few slides of the
  presentation are designed to be used to
  facilitate class discussion (I create lists of
  the answers to each question on the board as the
  class provides them)

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License information

• This work is licensed under the Creative Commons
  Attribution-NonCommercial-ShareAlike 3.0 License.
  To view a copy of this license, visit
  http//creativecommons.org/licenses/by-nc-sa/3.0/u
  s/ or send a letter to Creative Commons, 171
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  California, 94105, USA.
• The slides in this presentation were originally
  created by Marc C. Perkins (http//faculty.orangec
  oastcollege.edu/mperkins).
• You are free to use, modify, and distribute these
  slides according to the terms of the Creative
  Commons license (e.g., you must attribute the
  slides, no commercial uses are allowed, and
  future distributions must be licensed under a
  similar license).
• Attribution should be given to Marc C. Perkins
  (and any later editors), including a link back to
  Marcs current website. This applies both while
  distributing the slides and during use of the
  slides attribution during use can be satisfied
  by, for instance, placing small text on at least
  one of the slides that has been shown (see below
  for an example).

Slides in this presentation based on those
created by Marc C. Perkins. http//faculty.orangec
oastcollege.edu/mperkins
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History

• August 2007 Marc Perkins released first version.
  http//faculty.orangecoastcollege.edu/mperkins

(If you modify these slides and redistribute
them, add your information to the list)