?????(Flow Cytometer) ???? - PowerPoint PPT Presentation

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?????(Flow Cytometer) ????

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Title: Flow Cytometry) Author: Chscist Last modified by: lilei Created Date: 3/1/2005 12:16:44 PM Document presentation format – PowerPoint PPT presentation

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Title: ?????(Flow Cytometer) ????


1
?????(Flow Cytometer)????
  • ??????????
  • ??????????????
  • 2005?3? ???

2
????
  • ???????
  • ??????????
  • ??????????

3
????????
????????
BD FACSVantage
BD-Calibur
4
  • ?????????

5
?FCM???
  • ???????,???(?????)???????????????,??????
    ?????????,???????,?????????????????

6
  • ??
  • ??????????????

7
(No Transcript)
8
  • ????
  • (1)????????????
  • (2)????
  • ???99??
  • ?????1/10000????????
  • ???????

(3)???(????). ??150,000?/ ?
??100,000?/?
9
  • ????
  • ????
  • ???????
  • ????
  • ???????
  • ??????
  • ??

70????????????? ?? Becton-Dickson Coulter-Beck
man DOCytomation ?????????????
10
?????????
?????
???????
???
????
11
??????
12
?????
?????,??,?????PMT??????????
13
????
???
FSC SSC FL1 FL2 FL3
???
?? ?? ?? ?? ??
????,????
(??,??,??)
????,????
14
??????????
  • ?????

???
????
15
?????????????????
16
  • ????

???????????????
???? ??????
?????????? ???????????,???????
17
PIAnnexin-V??????
DNA??-PI
Annexin-V
18
PEFITC???????????????
?????(linear)
????? (linear)
19
  • ????

20
??????????
  • ??
  • ??????????????
  • ????0.2uM-300uM?
  • ????
  • ????
  • (1) ??????
  • (2)??
  • (3)??
  • (4)???????,?????
  • ?????
  • ??????????????????????

21
  • ???????????????????
  • 1)????
  • ??,?????,??????,??,???????
  • 2)??????
  • ????
  • ??????,???,???
  • ?????H(???PH?),Na,K,Ca2(????
  • ?? ?)?
  • ??DNA??,DNA??,DNA??,RNA,??????
  • ????
  • ??????????
  • ??
  • ??????

22
???? ??? ???? ???? ???? ??? ??????? ?????
..
????????????????????????,?????????????
23
??????
???
DNA ??
G1
S phase
M
G0/1
G2
G1
G2/M
S
1
2 1
DNA??
??????
24
???????
2
1
4
3
?????
5
1 / 3
6
Hoechst 33258 (AT)
X 8
7 recip 9-22
13
Y 18
9-12
14 15 16 17
20
21
19
22
Ph
Chromomycin A3 (GC)
25
????????????
  • ??BD FACSVantage SE
  • ????10,000?/?,??7,000?/???488nm,UV???????
  • ??1406
  • ??????64839306 ? 64862503
  • ????lcc_at_moon.ibp.ac.cn
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