Recombinant DNA technology is used in all aspects of biology:

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Recombinant DNA technology is used in all aspects
of biology
Medicine

1. Insulin,
2. HGH
3. Vaccines
4. Factor 8

e) Forensic DNA fingerprinting, f) Gene
therapy g) Disease diagnosis
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Medicine

1. Insulin,
2. HGH
3. Vaccines
4. Factor 8

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Forensic DNA fingerprinting
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Production of valuable pharmaceuticals in
transgenic sheep milk
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Human genome project
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Agriculture Development of novel varieties of
plants

1. Increased protein and yield
2. Increased vitamin content
3. Pest and disease resistance.

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Agriculture Development of novel varieties of
plants

1. Increased protein and yield
2. Increased vitamin content
3. Pest and disease resistance.

Golden rice with increased levels of vitamin A
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This technology is called
Recombinant DNA, Gene Cloning, Genetic
Engineering, Transgenic technology
Ability to take a gene from one organism, and
stably insert it into another with a view to
expression
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Recombinant DNA technology involves
1) A method of cutting DNA molecules
2) A method for isolating DNA molecules
2) A method of joining DNA molecules
3) A method of introducing DNA molecules into
cells and organisms
4) A method of identifying recombinants
5) A method to drive expression of the foreign
gene in an alien environment.
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1) A method of cutting DNA molecules
Restriction Enzymes.
Restriction enzymes are bacterial enzymes which
have evolved to protect Bacteria from phage
attack.
Incoming phage DNA is cleaved, but bacterial DNA
is not because it is methylated.
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Restriction enzymes are named after the bacterium
from which they were first isolated
Eco R1 Escherichia coli G
A A T T C 6 bp
Bam H1 Bacillus amyloliquefaciens G G A T C C
6 bp
Sau 3A Staphylococcus aureus G A T C
4 bp
Not 1 Nocardia
- G C G G C C G C 8 bp
Hind 3 Haemophilus influenzae A A G C
T T 6 bp.
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Restriction enzymes cleave DNA at SPECIFIC
sequences
To give Blunt (flush) ends and
Sticky (cohesive) ends