F?S????S????? ????S MALDI

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F?S????S????? ????S MALDI TOF

• ?a??a??? G??????

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???sd????sµ?? ????µe???? ??????

• ???sd????sµ?? ????a??? ??????
• ???µat???af?a ?????s?? ???t??
• ?????as? F?t??
• Osµ?µet??a
• ???d?µet??a
• ???sd????sµ?? ????????a? ????µe??? ????d??
• Fasµat?s??p?a ??R
• ???sd????sµ?? ?e????? ?µ?d??
• ??t??d?t?s?
• Fasµat?s??p?a NMR
• Fasµat?s??p?a FT - IR
• ???sd????sµ?? ?a?a??t?ta?
• St???e??µet???? ?????s?
• Fasµat?s??p?a NMR
• Fasµat?s??p?a FT - IR

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???sd????sµ?? ?? µe Fasµat?s??p?a ???a? (Mass
Spectroscopy)

• ???s?µ?p??e?ta? st?? a????s? ?? ta te?e?ta?a 50
  ?????a
• ?p?? s?et??? ???a??????a
• ?e???? a???ße?a
• G??????
• a???...

• ? ?F????G? ??S ????? ???????S???? S? S??T?????
  ???????? ??? ??G??? ????????!
• ???? ?aµ???? ?t?t???t?ta? ?
• Te?µ???? ?st??e?a? t?? e??se?? a?t??

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?e?t??se?? ?a? pa?a??a??? t?? te?????? MS.

• ?e?????? ?p??? ????sµ??
• Chemical Ionization
• Secondary-Ion Mass Spectroscopy
• Field Desorption
• Fast Atom Bombardment
• Matrix-assisted Laser Desorption/Ionization -
  Mass Spectroscopy

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Matrix-assisted Laser Desorption/Ionization
????d?? MALDI
?????e?t?? TOF-MS
Time Of Flight

• ?p?t??pe? t?? p??sd????sµ? µa??? s???et????
• p???µe??? µe
• ?? gt 200.000 Daltons (Da) µ?s? ????sµ?? ?a?
  atµ?p???s??,
• ????? ?µ?? t?? ap????d?µ?s? t???,
• ??????a
• µe a???ße?a
• p??sd????sµ?? µe????? a???µ?? ?a?a?t???st???? t??
  p???µe????

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?e????? ?p??? ????sµ?? MALDI

• ???µ??? ???s?? - µ?t?a? ?a? p???µe???? se
  ?at?????? d?a??t?.( Cmatrix 100 x Cpolymer )
• ?p?µ?????s? d?a??t?, d?µ??????a ?µ??e????
  ???st????? µe ?µ???µ??f? d?asp??? µ?????
  p???µe????
• ?etaf??? t?? e????e?a? t?? pa?µ?? t?? a?t??a?
  laser st?? µ?t?a, µe???? atµ?p???s? t?? µe
  a??????? µetaf??? ????t?? µ????? p???µe???? st??
  a???a f?s? ?a? pa?a???? f??t?sµ???? a??s?d??
  p???µe????.

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???? ?e?t?????a? fasµat????f?? MALDI - TOF
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S??p?? ???s?µ?p???s?? µ?t?a?

• ?p????f?s? t?? e????e?a? t?? a?t??a? t?? laser,
  ?a? ep?µ???? ap?f??? ap????d?µ?s?? t??
  p???µe????.
• ?s???? ap????f?s? st? µ???? ??µat?? ?e?t?????a?
  t?? laser, st? ?p??? ? a?a??t?? de? ap????f?
  s?µa?t???.
• ?p?µ???s? t?? e??? µ????? t?? p???µe???? ap? ta
  ???a.
• ??a??st?p???s? t?? d?aµ???a??? a????ep?d??se??
  p??a ap? a?t?? µ?t?a? a?a??t?, ??a µe??s?
  e????e?a? e?a???s??.
• S?µµet??e? st?? d?µ??????a t?? ???t?? e?te sa?
  µ?s? p??t????s??(a????e?s? ?at???t??) ? µ?s?
  ap?p??t????s?? (a????e?s? a????t??).
• ?????e?s? s???e?t??se?? t?? t???? t?? picomoles ?
  ?a? µ????te?e?.

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?d??t?te? ???s?? - ??t?a?

• ?a e??a? d?a??t? se d????µa µe t?? a?a??t?.
• ?a ??e? µe???? s??te?est? ap????f?s?? st?
  s???e???µ??? µ???? ??µat?? ?e?t?????a? t?? laser.
  
• ? µ?t?a ?a p??pe? ?a µ?? eµfa???e? ??µ???
  d?ast???t?ta µe t?? a?a??t?.
• ?a ??e? µ???? t?s? e?????s??

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??t?e? MALDI
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??t?e? MALDI
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??t?e? MALDI
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??ad??as?a ????sµ?? ?e??µat??
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Fasµat????f?? ???a? ?????? ?t?s?? (TOF MS)

• St????e? t?? ?e?t?????a t?? st? s??s? µeta??
  ????? µ??a? p??? f??t?? e??? f??t?sµ????
  s?µat?d??? ?a? ?????? p?? apa?te?ta? ??a ?a
  d?a??se? µ?a s???e???µ??? ap?stas? ?ta?
  ep?ta????e? d?a µ?s?? sta?e??? ?µ??e???? ped???.

?p?? m µ??a s?µat?d???, e ??e?t???? f??t??, E
??tas? as???µe??? ??e?t??stat??? ped??, d
µ???? pe?????? ep?t????s?? L µ???? pe??????
e?e??e??? ??e?t????? ped??? ?a? Vo efa?µ???µe??
d??aµ???.
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??e??e?t?µata a????e?t? TOF MS

• Te???t??? ??e? ?pe??? e???? t?µ?? a????e??µe???
  µa???.
• ?da????? ?p?? ????µe ????sµ? p???a???µe?? ap?
  pa?µ? ? a?t?? e??a? ????ta???? pe?????sµ????.
• ????????µ??? f?sµa µa??? ??a ???e pe??pt?s?
  ????sµ??.
• ????? a???ße?a ???? ?????? ta??t?ta? µet?ßas??
  ???t?? p??? t?? a????e?t?.
• ?e? ?p???e? a????? s???s?? t?? a?t??a? ???t??.
• ?? f?sµa ?aµß??eta? ?a? µe e?a??et??? µ?????
  p?s?t?te? de??µat??(lt10-18 mole sta p?? s??????a
  ???a?a).
• S?et??? ?aµ??? ??st??.

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???? ?e?t?????a? TOF-?S e?????aµµ?? ????s??
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Sf??µata st?? TOF MS e?????aµµ?? ????s??

• ?ata??µ? st? ?????.

• ?ata??µ? st? ????.

• ?ata??µ? ????t???? e????e?a?.

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??a??st?p???s? sf??µat??

• ?????e?t?? a????as?? ???t??
• ?p??? ped???

• ??p??? ped???
• ?et?a??????? ped???

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?etat??p? Fasµ?t?? MALDI-TOF MS
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??a???? ?a? epe?e??as?a ded?µ????
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??a???? ?a? epe?e??as?a ded?µ????
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????µe?? S?µp????s??
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????µe?? S?µp????s??
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THE END