another example:

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another example Carbonic Anhydrase
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Zn
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Active site in a -carbonic anhydrase
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Active site and mechanism in b -carbonic anhydrase
B(GLU)
HB(GLU)
START
Nucleophilic attack on CO2
Charge stabilization
deprotonation
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Carbonic Anhydrase is critically important in
global C- cycle
CO2 --gt glucose
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Carbonic Anhydrase important in global C- cycle
because catalyzes both directions! Phytoplankton
do this HCO3- ? CO2 Diatoms do this CO2 ?
HCO3-
CO2 lt-- CO32-
CO2 --gt CO32-
CA importance means these critters are growth
limited when theres too little Zn, can use Cd
and Co!!
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Acid/base chemisty in catalysis of Mo enzymes
oxidation of SO32-
ditto
See in this step how Mo charge affects H2O acidity
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