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Laboratory Safety


Laboratory Safety Why Lab Safety ... Regulations that Affect You Occupational Exposures to Hazardous Chemicals in Laboratories ... weather emergency or laboratory ... – PowerPoint PPT presentation

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Title: Laboratory Safety

Laboratory Safety
Why Lab Safety?
  • Protect yourself from laboratory hazards
  • Protect others from laboratory hazards
  • Comply with State and Federal regulations

Laboratory Safety TrainingOverview
  • Introduction to the Lab Standard
  • Responsibilities
  • Protecting yourself from Laboratory hazards
  • Toxicity/Compounds of unknown toxicity
  • Spills and Spill Response
  • Mandatory work practices

Regulations that Affect You
  • Occupational Exposures to Hazardous Chemicals in
  • The OSHA Lab Standard (29CFR1910.1450)
  • HAZWOPPER (Chemical Spills)
  • Hazardous Waste Operations Standard
  • Bloodborne Pathogens
  • (29CFR1910.)

Contents of the Lab Standard
  • Scope and Application
  • Definitions
  • Permissible Exposure Determination
  • Chemical Hygiene Plan
  • Employee Information and Training
  • Medical Consultation/Examinations
  • Hazard Identification
  • Use of Respirators
  • Recordkeeping
  • Dates
  • Appendices

Scope and application of the Lab Standard
  • Applies only to laboratories . workplace where
    relatively small quantities of chemicals are used
    on a non-production basis
  • Supercedes for labs, the requirements in
    29CFR1910 subpart Z , but requires protection to
    PEL limits and prohibition of eye and skin
    contact as specified by other OSHA standards

Permissible Exposure Limitsand Exposure
  • The employer shall assure that laboratory
    employees exposure to OSHA regulated substances
    do not exceed the Permissible Exposure Limits set
    in 29 CFR1910, subpart Z
  • Employer must monitor employees exposure to
    substances regulated by an OSHA Standard if there
    is reason to believe that exposure levels for
    that substance routinely exceed action levels or

Employer Responsibilities
  • Keep records of employee exposures to regulated
    hazardous chemicals
  • Provide Information and Training
  • Prepare, implement and maintain a written
    Chemical Hygiene Plan (CHP)
  • Provide Personal Protective Equipment (PPE)
  • Hazard Identification
  • Provide for medical consultations\exams
  • Recordkeeping

Risk Assessment
  • As a research scientist, you are responsible for
    assessing the potential Safety and Environmental
    hazards presented by your work
  • When unusual or uncontrolled hazards may be
    predicted, you should consult with your
    supervisor and the Safety and Environmental

New Equipment PurchaseRisk Assessment for Hazards
  • When you contemplate purchasing new equipment for
    your lab, please
  • use the risk assessment guide below to identify
    potential hazards.
  • Do you have space to use this equipment safely?
  • Does this equipment require special ventilation?
  • Does this equipment contain any radioactive
    sources or lasers?
  • Are there any special PPE (personal protective
    equipment) requirements when using this
  • Are all points where injury could occur guarded?
  • Do you require any special heating, cooling or
    electrical components to use this equipment?
  • If you have questions regarding hazards youve
  • please call Safety.

Chemical Hygiene Plan
  • What is a Chemical Hygiene Plan?
  • Where can you find it?
  • Why is important to you?

Use of Chemicalsand Biological Agents
  • Know the properties of chemicals and biological
    agents you use before you use or transport them
  • Toxicity
  • Flammability
  • Reactivity/Incompatibilities
  • Corrosive
  • Unstable
  • Radioactive
  • Clean up procedures

Where to find information on Chemical Hazards
  • Where do you look to find information on new
    chemicals BEFORE you use them?
  • Books available on Site
  • Merck Index
  • Aldrich Catalog
  • Prudent Practices in the Laboratory
  • Saxs Dangerous Properties of Industrial Materials

Internet Sources for Chemical Hazard Information
  • American Chemical Society (ACS)
  • http//
  • Howard Hughes Institute
  • American Biological Safety Association
  • National Institute of Occupational Safety and
    Health (NIOSH)
  • Center for Disease Control (CDC)

MSDSMaterial Safety Data Sheets
  • MSDS provide you with the following
  • Chemical and Physical properties
  • Toxicity Information
  • Computability/Incompatibility
  • Appropriate spill and fire response
  • and much more information......................

Chemical Storage
  • Acids are incompatible with bases, flammable
    solvents, oxidizers
  • Cyanides should stored separately from acids
  • Water reactive materials should be stored
  • Flammable materials with very low flashpoints
    should be stored in an explosion proof
  • Peroxidizable chemicals must be dated when
    opened, disposed of when required per safety
  • Keep on hand only those chemicals that you have
    room to store properly.

Chemical Spills Nuisance Spills
  • Spills of less than 4 L. of material that you
    know the hazards of and are comfortable cleaning
  • Assess the hazard
  • Wear appropriate PPE
  • If you are unsure of the hazard of a spill
  • or need assistance with PPE selection,
  • call the Safety Office

Chemical Spills Potentially Hazardous Spills
  • Spills of
  • greater than 4L
  • smaller spills of materials of
  • low LD50,
  • carcinogens,
  • flammable
  • unknown toxicity

Emergency Lab Evacuation
  • In the event of a fire, severe weather emergency
    or laboratory evacuation scientists should
  • stabilize reactions in progress
  • close fume hood sashes
  • notify safety if any unusual hazards exist

HazMat Team
  • 30 Team members, 10 First Responders from
    Facilities, Engineering, Safety and Scientific
  • First Responders go to the location of all
    shower, eyewash, hood and ventilation alarms.
    They evaluate and decide how to deal with each

Fire Extinguishers in Your Lab
  • Know where all fire
  • extinguishers are located in your lab
  • Visually inspect them each time you walk through
    the lab
  • are extinguishers in proper location?
  • has extinguisher been discharged?
  • Call Maintenance
  • if extinguishers need to be replaced or repaired

  • Personal Protective Equipment (PPE)
  • Do you know what PPE is appropriate for the work
    you are doing?
  • What PPE is provided?
  • Gloves - rubber, butyl, latex
  • Eye Protection - glasses, goggles, faceshields
  • Labcoats, aprons, scrubs
  • Safety shoes
  • Respirators

  • select gloves appropriate for the task
  • check gloves for leaks
  • double glove if necessary
  • be alert to unusual sensations in your hands
  • do not touch your face, telephone, etc with
    contaminated gloves
  • use clean hand / dirty hand technique

Safety GlassesEye protection must be worn in
any area where there is the potential for eye
  • All eye protection used must be ANSI approved
    (Z87 is stamped on the sidebar of ANSI
    approved eyewear)
  • If your prescription glasses are not ANSI
    approved, you must wear safety glasses, safety
    goggles or a full face shield over them

Available Eye Protection
  • Safety glasses
  • Splash goggles
  • Full face shields
  • All are available
  • Prescription safety glasses are available by
    contacting Occupational Health

Respirator UseRespirator Standard 29CFR1910.134
  • Required under the Standard
  • exposures exceed the exposure limit
  • Required by the Department
    (Not required under the standard)
  • Not Required

Respirator Use when not required by the Standard
or the Department
  • Respirators are an effective method of protection
    against designated hazards when properly selected
    and worn.
  • Voluntary use of disposable respirators is
    encouraged where they will provide an additional
    level of comfort and protection for Colleagues.
  • If a respirator is used improperly or not kept
    clean, the respirator can become a hazard to you.

Proper Use of Respirators
  • When using respirators that are provided for
    voluntary use, you need to take the following
    precautions to be sure that the respirator itself
    does not present a hazard
  • Read and follow all instructions provided by the
    manufacturer on use, maintenance, cleaning and
    care, and warnings regarding the limitations of
    the respirator
  • Do not wear your respirator into atmospheres
    containing contaminants for which your respirator
    is not designed to prevent against. For example,
    a respirator designed to filter dust particles
    will not protect you against gasses or vapors
  • Keep track of your respirator so that you do not
    mistakenly use someone elses respirator.

Obtaining a Respirator
  • Disposable respirators for nuisance levels of
    particulate matter, dust, dirt and dander are
    available in the apparatus storeroom
  • Respirators for use when required by the Standard
    are available by contacting the Safety Office.

Biosafety Cabinets
  • Class I and IIa,b cabinets, when used in
    conjunction with good microbiological techniques,
    provide an effective containment system for
    moderate to high risk microorganisms.
  • ClassI protects worker and environment
  • Class II protects worker, environment and
    research materials in the cabinet
  • Class IIa exhausts air back into the room do
    not use with volatile or toxic chemicals
  • Class IIb is hard ducted to the facility exhaust
  • All fume hoods and Biosafety cabinets are
    inspected yearly

Fume HoodsThe most expensive piece of real
estate in your lab!
  • Use Properly
  • keep sashes down
  • dont store flammable liquids, equipment in hood
  • limit traffic behind you

Mercury Spills
  • All mercury spills should be cleaned up in a
    timely fashion
  • To clean Hg spills, use the mercury vacuum
  • Call Safety or Environmental if you have any

  • rinse all visible residues and chemicals from
  • decontaminate/chemically destroy any biologically
    active or noxious materials before sending items
    to glassware
  • ENSURE that no sharps go in glassware bins
  • do not overload glassware bins
  • visit Glasswash facility

  • The dose determines level of toxicity
  • Dose Concentration X exposure Time
  • Acute vs. Chronic
  • alcohol consumption
  • Local vs. Systemic
  • pet flea shampoo

Toxicology Definitions
  • PEL-Permissible Exposure Level
  • Levels established by OSHA for personnel exposure
    to air contaminants.
  • IDLH-Imminently Dangerous to Life and Health
  • NIOSH recommendation refers to acute respiratory
    exposure that poses an immediate threat of loss
    of life or immediate or delayed irreversible
    effects on health

Routes of Exposure
  • Inhalation
  • Ingestion
  • Skin Absorption
  • Injection

Toxicity Definitions
  • Asphyxiant - prevents oxygen from reaching body
  • carbon monoxide, carbon dioxide.
  • Allergen/Sensitizer - causes allergic reaction
    which can be lethal (anaphylaxsis)
  • DCC, isocyanates, organophosphates, heavy metals
  • Carcinogen - known to, or suspected of, causing
  • benzene
  • Teratogen - reproductive toxins which may do
    damage to the fetus
  • dioxins, DES
  • Mutagen - reproductive toxins which may damage or
    interfere with genetic material

Select Carcinogenslisted in your Safety Manual
  • OSHAs Thirteen Carcinogens
  • NTP listed Known to be Carcinogens
  • IARC Group 1 carcinogenic to humans
  • IARC Group 2A or 2B or NTP designated
    reasonably anticipated to be carcinogensthat
    cause tumor incease

Working with Select Carcinogens
  • Discuss with your supervisor
  • Follow procedures outlined in the CHP
  • Follow departmental procedures
  • Ensure that designated area is defined and used,
    ventilation and all appropriate PPE is being used

Working with Compounds of Unknown Toxicity
  • These are chemicals for which there are no known
    statistically significant studies to establish
    their toxicity
  • 1. Use a designated work area, isolate items used
    there decontaminate the area when work is
  • 2. Use local exhaust ventilation
  • 3. Use appropriate PPE and wash hands often

  • Women who are pregnant are encouraged to discuss
    work assignments with their supervisor and to
    seek alternate work assignments if the colleague
    believes the potential for exposure to teratogens
  • If alternative work assignments cannot be agreed
    upon, the colleague and supervisor should
    contact the Safety Office.

Mandatory Safety Rules
  • 1. Comply with Emergency Evacuation procedures.
  • 2. Access to eyewash/drench hoses, safety
    showers, and fire extinguishers must be kept
  • 3. Eating and drinking in the laboratories and
    animal rooms is forbidden.
  • 4. Adequate eye protection must be worn in areas
    where there is the potential for eye injury.
  • 5. Open toed shoes and sandals are not
    acceptable footwear in laboratories or animal
  • 6. All fires that cannot be extinguished with a
    fire extinguisher must be reported immediately.
  • 7. Fires that have been extinguished must be
    reported to your area supervisor, the Safety
    Office and to the Maintenance Department.

  • 8. Immediately report any work related illness
    or injury to your Supervisor and the Occupational
    Health Department.
  • 9. Tasks that present unusual hazards must be
    reviewed with the appropriate supervisor before
    they are conducted.
  • 10. Labels on containers must not be defaced,
    and all containers of chemicals stored in common
    areas must be labeled as to the contents,
    hazards, name of owner and date.
  • 11. Any chemicals, biological materials
    experimental compounds, or other hazardous
    materials must be shipped by CBI or Shipping and
  • 12. When working with select carcinogens, comply
    with all safety procedures.
  • 13. Pipetting by mouth is forbidden.
  • 14. Is anyone in this room still awake?
  • 15. Gas cylinders must be secured whether in use
    or stored. Regulators must be removed and caps
    used when moving cylinders.
  • 16. Materials with offensive odors must be
    decontaminated before disposal.
  • 17. Do not modify electrical equipment yourself!
    The electrical shop will modify or fix
    electrical equipment for you SAFELY.

Last, not Least.......
  • Before you leave your work area each day, check
    the area to ensure
  • Overnight operations are properly set-up and
    waterlines are adequately clamped
  • Heat sources are turned off
  • Hoods sashes are closed
  • Chemicals and biological agents are stored