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Laboratory Safety

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Laboratory Safety Why Lab Safety ... Regulations that Affect You Occupational Exposures to Hazardous Chemicals in Laboratories ... weather emergency or laboratory ... – PowerPoint PPT presentation

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Title: Laboratory Safety


1
Laboratory Safety
2
Why Lab Safety?
  • Protect yourself from laboratory hazards
  • Protect others from laboratory hazards
  • Comply with State and Federal regulations

3
Laboratory Safety TrainingOverview
  • Introduction to the Lab Standard
  • Responsibilities
  • Protecting yourself from Laboratory hazards
  • Toxicity/Compounds of unknown toxicity
  • Spills and Spill Response
  • Mandatory work practices

4
Regulations that Affect You
  • Occupational Exposures to Hazardous Chemicals in
    Laboratories
  • The OSHA Lab Standard (29CFR1910.1450)
  • HAZWOPPER (Chemical Spills)
  • Hazardous Waste Operations Standard
    (29CFR1910.120)
  • Bloodborne Pathogens
  • (29CFR1910.)

5
Contents of the Lab Standard
  • Scope and Application
  • Definitions
  • Permissible Exposure Determination
  • Chemical Hygiene Plan
  • Employee Information and Training
  • Medical Consultation/Examinations
  • Hazard Identification
  • Use of Respirators
  • Recordkeeping
  • Dates
  • Appendices

6
Scope and application of the Lab Standard
  • Applies only to laboratories . workplace where
    relatively small quantities of chemicals are used
    on a non-production basis
  • Supercedes for labs, the requirements in
    29CFR1910 subpart Z , but requires protection to
    PEL limits and prohibition of eye and skin
    contact as specified by other OSHA standards

7
Permissible Exposure Limitsand Exposure
Monitoring
  • The employer shall assure that laboratory
    employees exposure to OSHA regulated substances
    do not exceed the Permissible Exposure Limits set
    in 29 CFR1910, subpart Z
  • Employer must monitor employees exposure to
    substances regulated by an OSHA Standard if there
    is reason to believe that exposure levels for
    that substance routinely exceed action levels or
    PELs.

8
Employer Responsibilities
  • Keep records of employee exposures to regulated
    hazardous chemicals
  • Provide Information and Training
  • Prepare, implement and maintain a written
    Chemical Hygiene Plan (CHP)
  • Provide Personal Protective Equipment (PPE)
  • Hazard Identification
  • Provide for medical consultations\exams
  • Recordkeeping

9
Risk Assessment
  • As a research scientist, you are responsible for
    assessing the potential Safety and Environmental
    hazards presented by your work
  • When unusual or uncontrolled hazards may be
    predicted, you should consult with your
    supervisor and the Safety and Environmental
    Departments

10
New Equipment PurchaseRisk Assessment for Hazards
  • When you contemplate purchasing new equipment for
    your lab, please
  • use the risk assessment guide below to identify
    potential hazards.
  • Do you have space to use this equipment safely?
  • Does this equipment require special ventilation?
  • Does this equipment contain any radioactive
    sources or lasers?
  • Are there any special PPE (personal protective
    equipment) requirements when using this
    equipment?
  • Are all points where injury could occur guarded?
  • Do you require any special heating, cooling or
    electrical components to use this equipment?
  • If you have questions regarding hazards youve
    identified,
  • please call Safety.

11
Chemical Hygiene Plan
  • What is a Chemical Hygiene Plan?
  • Where can you find it?
  • Why is important to you?

12
Use of Chemicalsand Biological Agents
  • Know the properties of chemicals and biological
    agents you use before you use or transport them
  • Toxicity
  • Flammability
  • Reactivity/Incompatibilities
  • Corrosive
  • Unstable
  • Radioactive
  • Clean up procedures

13
Where to find information on Chemical Hazards
  • Where do you look to find information on new
    chemicals BEFORE you use them?
  • Books available on Site
  • Merck Index
  • Aldrich Catalog
  • Prudent Practices in the Laboratory
  • Saxs Dangerous Properties of Industrial Materials

14
Internet Sources for Chemical Hazard Information
  • American Chemical Society (ACS)
  • http//dchas.cehs.siu.edu
  • Howard Hughes Institute
  • American Biological Safety Association
  • National Institute of Occupational Safety and
    Health (NIOSH)
  • Center for Disease Control (CDC)

15
MSDSMaterial Safety Data Sheets
  • MSDS provide you with the following
  • Chemical and Physical properties
  • Toxicity Information
  • Computability/Incompatibility
  • Appropriate spill and fire response
  • and much more information......................

16
Chemical Storage
  • Acids are incompatible with bases, flammable
    solvents, oxidizers
  • Cyanides should stored separately from acids
  • Water reactive materials should be stored
    separately
  • Flammable materials with very low flashpoints
    should be stored in an explosion proof
    refrigerator
  • Peroxidizable chemicals must be dated when
    opened, disposed of when required per safety
    manual.
  • Keep on hand only those chemicals that you have
    room to store properly.

17
Chemical Spills Nuisance Spills
  • Spills of less than 4 L. of material that you
    know the hazards of and are comfortable cleaning
    up
  • Assess the hazard
  • Wear appropriate PPE
  • If you are unsure of the hazard of a spill
  • or need assistance with PPE selection,
  • call the Safety Office

18
Chemical Spills Potentially Hazardous Spills
  • Spills of
  • greater than 4L
  • smaller spills of materials of
  • low LD50,
  • carcinogens,
  • flammable
  • unknown toxicity

19
Emergency Lab Evacuation
  • In the event of a fire, severe weather emergency
    or laboratory evacuation scientists should
  • stabilize reactions in progress
  • close fume hood sashes
  • notify safety if any unusual hazards exist

20
HazMat Team
  • 30 Team members, 10 First Responders from
    Facilities, Engineering, Safety and Scientific
    Departments
  • First Responders go to the location of all
    shower, eyewash, hood and ventilation alarms.
    They evaluate and decide how to deal with each
    situation.

21
Fire Extinguishers in Your Lab
  • Know where all fire
  • extinguishers are located in your lab
  • Visually inspect them each time you walk through
    the lab
  • are extinguishers in proper location?
  • has extinguisher been discharged?
  • Call Maintenance
  • if extinguishers need to be replaced or repaired

22
  • Personal Protective Equipment (PPE)
  • Do you know what PPE is appropriate for the work
    you are doing?
  • What PPE is provided?
  • Gloves - rubber, butyl, latex
  • Eye Protection - glasses, goggles, faceshields
  • Labcoats, aprons, scrubs
  • Safety shoes
  • Respirators

23
Gloves
  • select gloves appropriate for the task
  • check gloves for leaks
  • double glove if necessary
  • be alert to unusual sensations in your hands
  • do not touch your face, telephone, etc with
    contaminated gloves
  • use clean hand / dirty hand technique

24
Safety GlassesEye protection must be worn in
any area where there is the potential for eye
injury
  • All eye protection used must be ANSI approved
    (Z87 is stamped on the sidebar of ANSI
    approved eyewear)
  • If your prescription glasses are not ANSI
    approved, you must wear safety glasses, safety
    goggles or a full face shield over them

25
Available Eye Protection
  • Safety glasses
  • Splash goggles
  • Full face shields
  • All are available
  • Prescription safety glasses are available by
    contacting Occupational Health

26
Respirator UseRespirator Standard 29CFR1910.134
  • Required under the Standard
  • exposures exceed the exposure limit
  • Required by the Department
    (Not required under the standard)
  • Not Required

27
Respirator Use when not required by the Standard
or the Department
  • Respirators are an effective method of protection
    against designated hazards when properly selected
    and worn.
  • Voluntary use of disposable respirators is
    encouraged where they will provide an additional
    level of comfort and protection for Colleagues.
  • If a respirator is used improperly or not kept
    clean, the respirator can become a hazard to you.

28
Proper Use of Respirators
  • When using respirators that are provided for
    voluntary use, you need to take the following
    precautions to be sure that the respirator itself
    does not present a hazard
  • Read and follow all instructions provided by the
    manufacturer on use, maintenance, cleaning and
    care, and warnings regarding the limitations of
    the respirator
  • Do not wear your respirator into atmospheres
    containing contaminants for which your respirator
    is not designed to prevent against. For example,
    a respirator designed to filter dust particles
    will not protect you against gasses or vapors
  • Keep track of your respirator so that you do not
    mistakenly use someone elses respirator.

29
Obtaining a Respirator
  • Disposable respirators for nuisance levels of
    particulate matter, dust, dirt and dander are
    available in the apparatus storeroom
  • Respirators for use when required by the Standard
    are available by contacting the Safety Office.

30
Biosafety Cabinets
  • Class I and IIa,b cabinets, when used in
    conjunction with good microbiological techniques,
    provide an effective containment system for
    moderate to high risk microorganisms.
  • ClassI protects worker and environment
  • Class II protects worker, environment and
    research materials in the cabinet
  • Class IIa exhausts air back into the room do
    not use with volatile or toxic chemicals
    (Mercaptoethanol)
  • Class IIb is hard ducted to the facility exhaust
    system
  • All fume hoods and Biosafety cabinets are
    inspected yearly

31
Fume HoodsThe most expensive piece of real
estate in your lab!
  • Use Properly
  • keep sashes down
  • dont store flammable liquids, equipment in hood
  • limit traffic behind you

32
Mercury Spills
  • All mercury spills should be cleaned up in a
    timely fashion
  • To clean Hg spills, use the mercury vacuum
  • Call Safety or Environmental if you have any
    questions

33
Glassware
  • rinse all visible residues and chemicals from
    glassware
  • decontaminate/chemically destroy any biologically
    active or noxious materials before sending items
    to glassware
  • ENSURE that no sharps go in glassware bins
  • do not overload glassware bins
  • visit Glasswash facility

34
Toxicity
  • The dose determines level of toxicity
  • Dose Concentration X exposure Time
  • Acute vs. Chronic
  • alcohol consumption
  • Local vs. Systemic
  • pet flea shampoo

35
Toxicology Definitions
  • PEL-Permissible Exposure Level
  • Levels established by OSHA for personnel exposure
    to air contaminants.
  • IDLH-Imminently Dangerous to Life and Health
  • NIOSH recommendation refers to acute respiratory
    exposure that poses an immediate threat of loss
    of life or immediate or delayed irreversible
    effects on health

36
Routes of Exposure
  • Inhalation
  • Ingestion
  • Skin Absorption
  • Injection

37
Toxicity Definitions
  • Asphyxiant - prevents oxygen from reaching body
    tissue.
  • carbon monoxide, carbon dioxide.
  • Allergen/Sensitizer - causes allergic reaction
    which can be lethal (anaphylaxsis)
  • DCC, isocyanates, organophosphates, heavy metals
  • Carcinogen - known to, or suspected of, causing
    cancer
  • benzene
  • Teratogen - reproductive toxins which may do
    damage to the fetus
  • dioxins, DES
  • Mutagen - reproductive toxins which may damage or
    interfere with genetic material

38
Select Carcinogenslisted in your Safety Manual
  • OSHAs Thirteen Carcinogens
  • NTP listed Known to be Carcinogens
  • IARC Group 1 carcinogenic to humans
  • IARC Group 2A or 2B or NTP designated
    reasonably anticipated to be carcinogensthat
    cause tumor incease

39
Working with Select Carcinogens
  • Discuss with your supervisor
  • Follow procedures outlined in the CHP
  • Follow departmental procedures
  • Ensure that designated area is defined and used,
    ventilation and all appropriate PPE is being used
    properly.

40
Working with Compounds of Unknown Toxicity
  • These are chemicals for which there are no known
    statistically significant studies to establish
    their toxicity
  • 1. Use a designated work area, isolate items used
    there decontaminate the area when work is
    completed
  • 2. Use local exhaust ventilation
  • 3. Use appropriate PPE and wash hands often

41
Pregnancy
  • Women who are pregnant are encouraged to discuss
    work assignments with their supervisor and to
    seek alternate work assignments if the colleague
    believes the potential for exposure to teratogens
    exists.
  • If alternative work assignments cannot be agreed
    upon, the colleague and supervisor should
    contact the Safety Office.

42
Mandatory Safety Rules
  • 1. Comply with Emergency Evacuation procedures.
  • 2. Access to eyewash/drench hoses, safety
    showers, and fire extinguishers must be kept
    clear.
  • 3. Eating and drinking in the laboratories and
    animal rooms is forbidden.
  • 4. Adequate eye protection must be worn in areas
    where there is the potential for eye injury.
  • 5. Open toed shoes and sandals are not
    acceptable footwear in laboratories or animal
    rooms.
  • 6. All fires that cannot be extinguished with a
    fire extinguisher must be reported immediately.
  • 7. Fires that have been extinguished must be
    reported to your area supervisor, the Safety
    Office and to the Maintenance Department.

43
  • 8. Immediately report any work related illness
    or injury to your Supervisor and the Occupational
    Health Department.
  • 9. Tasks that present unusual hazards must be
    reviewed with the appropriate supervisor before
    they are conducted.
  • 10. Labels on containers must not be defaced,
    and all containers of chemicals stored in common
    areas must be labeled as to the contents,
    hazards, name of owner and date.
  • 11. Any chemicals, biological materials
    experimental compounds, or other hazardous
    materials must be shipped by CBI or Shipping and
    Receiving.
  • 12. When working with select carcinogens, comply
    with all safety procedures.
  • 13. Pipetting by mouth is forbidden.
  • 14. Is anyone in this room still awake?
  • 15. Gas cylinders must be secured whether in use
    or stored. Regulators must be removed and caps
    used when moving cylinders.
  • 16. Materials with offensive odors must be
    decontaminated before disposal.
  • 17. Do not modify electrical equipment yourself!
    The electrical shop will modify or fix
    electrical equipment for you SAFELY.

44
Last, not Least.......
  • Before you leave your work area each day, check
    the area to ensure
  • Overnight operations are properly set-up and
    waterlines are adequately clamped
  • Heat sources are turned off
  • Hoods sashes are closed
  • Chemicals and biological agents are stored
    properly
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