DNA EXTRACTION PowerPoint PPT Presentation

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Title: DNA EXTRACTION


1
DNA EXTRACTION
  • PROCEDURES

2
Obtain Cheek Cells
  • Gently Bite side of cheeks
  • Swish 3 mls of water in mouth and spit it back
    into the test tube.

3
Lyse the cells
  • Add 10 mls of the Lyse buffer solution to test
    tube

4
Denature Protein
  • Add 5 drops of PROTEASE to the solution
  • Heat at 50 degrees C for 10 minutes

5
Precipitate DNA
  • Add 10 mls of ethanol to
  • The test tube, slowly at a
  • 45 degree angle

6
Collect DNA
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