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## Machine Learning is based on Near Neighbor Set(s), NNS.

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Title: Machine Learning is based on Near Neighbor Set(s), NNS.

1
Database analysis can be broken down into 2
areas, Querying and Data Mining.
Data Mining can be broken down into 2 areas,
Machine Learning and Assoc. Rule
Mining Machine Learning can be broken down into
2 areas, Clustering and Classification. Clu
stering can be broken down into 2 types,
Isotropic (round clusters) and
Density-based Classification can be broken down
into to types, Model-based and
Neighbor-based
• Machine Learning is based on Near Neighbor
Set(s), NNS.
• Clustering, even density based, identifies near
neighbor cores 1st (disk shaped NNSs about a
center).
• Classification is continuity based and Near
Neighbor Sets (NNS) are the central concept in
continuity
• ??gt0 ??gt0 ? d(x,a)lt? ? d(f(x),f(a))lt?
where f assigns a class to a feature
vector, or
• ? ?-NNS of f(a), ? a ?-NNS of a in its
pre-image. f(Dom) categorical ??gt0 ?
d(x,a)lt??f(x)f(a)
• Using horizontal data, NNS derivation requires at
least one scan (at least O(n)).
• L? disk NNS can be derived using vertical-data in
O(log2n) yet usually Euclidean disks are
preferred. (Note Euclidean and Manhattan
coincide in Binary data sets).
• Our solution in a sentence Circumscribe the
desired Euclidean-disk with functional-contours,
(sets of the type f -1(b,c ) until the
intersection is scanable, then scan it for
Euclidean-disk membership.
• Advantage intersection can be determined before
scanning - create and AND functional contour
P-trees.

2
Contours ? fR(A1..An) ? Y
• and ? S?Y, the f-contour(S) f-1(S) Equiv.,
Af-contour(S) Select x1..xn From R Where
x.Aff(x1..xn)

If Sa, we use f-Isobar(a) equiv. Af-Isobar(a)
If f is a local density and Sk is a partition
of Y, f-1(Sk) partitions R. (eg, In OPTICS,
freachability distance, Sk is the partition
produced by intersections of graph-f wrt to a
walk of R and a horizontal line. A Weather map
use equiwidth interval partition of SReals
(barometric pressure or temperature contours). A
grid is the intersection partition with respect
to the dimension projection functions (next
slide). A Class is a contour under fR?C, the
class map. An L? ?-disk about a is the
intersection of the ?-dimension_projection
contours containing a.
3
GRIDs
fR?Y, ? partition SSk of Y, f-1(Sk)
S,f-grid of R (grid cellscontours)
If YReals, the j.lo f-grid is produced by
agglomerating over the j lo bits of Y, ? fixed
(b-j) hi bit pattern. The j lo bits walk
isobars of cells. The b-j hi bits
identify cells. (loextension / hiintention)
Let b-1,...,0 be the b bit positions of Y. The
j.lo f-grid is the partition of R generated by f
and S Sb-1,...,b-j Sb-1,...,b-j
(b-1)(b-2)...(b-j)0..0, (b-1)(b-2)...(b-j)1..1)
partition of YReals. If Ffh, the j.lo
F-grid is the intersection partition of the j.lo
fh-grids (intersection of partitions). The
canonical j.lo grid is the j.lo ?-grid
??dR?RAd ?d dth coordinate projection
j-hi gridding is similar ( the b-j lo bits walk
cell contents / j hi bits identify cells).
If the horizontal and vertical dimensions have
bitwidths 3 and 2 respectively
4
j.lo and j.hi gridding continued The
horizontal_bitwidth vertical_bitwidth b
iff j.lo grid (b-j).hi grid e.g.,
for hbvbb3 and j2
2.lo grid
1.hi grid
111 110 101 100
111 110 101 100
011 010 001 000
011 010 001 000
000 001 010 011 100 101 110 111
000 001 010 011 100 101 110 111
5
SOME Useful NearNeighborSets (NNS) Given a
similarity sR?R?Reals and a C?R
(i.e., s(x,y)s(y,x) s(x,x)?s(x,y) ?x,y?R)
Ordinal disks, skins and rings A disk(C,k) ? C
? disk(C,k)?C'k and s(x,C)?s(y,C)
?x?disk(C,k), y?disk(C,k) A skin(C,k)
disk(C,k)-C (skin
comes from s k immediate neighbors and is a kNNS
of C.) A ring(C,k) skin(C,k)-skin(C,k-1)
closeddisk(C,k)??alldisk(C,k)
closedskin(C,k)??allskin(C,k)
Cardinal disk, skins and rings The disk(C,r) ?
x?R s(x,C)?r also functional contour,
f-1(r, ?), where f(x)sC(x)s(x,C) The skin(C,r)
? disk(C,r) - C The ring(C,r2,r1) ?
disk(C,r2)-disk(C,r1) ? skin(C,r2)-skin(C,r1)
also functional contour, sC-1(r1,r2
Note closeddisk and closedskin(C,k) are
redundant, since closeddisk(C,k)
disk(C,s(C,y)) where y is any kth NN of C
L? skins skin?(a,k) x ?d, xd is one of the
k-NNs of ad - a local normalization?
6
fR(A1..An)?Y S?Y The (uncompressed)
Predicate-tree 0Pf, S is ? 0Pf,S(x)1(true) iff
f(x)?S
0Pf,S is called a P-tree for short and is just
the existential R-bit map of S ?R.Af
The Compressed P-tree, sPf,S is the compression
of 0Pf,S with equi-width leaf size, s, as
follows 1. Choose a walk of R
(converts 0Pf,S from bit map to bit
vector) 2. Equi-width partition 0Pf,S with
segment size, s (sleafsize, the last segment
can be short) 3. Eliminate and mask to 0, all
pure-zero segments (call mask, NotPure0 Mask or
EM) 4. Eliminate and mask to 1, all pure-one
segments (call mask, Pure1 Mask or UM)
(EMexistential aggregation UMuniversal
aggregation)
Compressing each leaf of sPf,S with leafsizes2
gives s1,s2Pf,S Recursivly, s1, s2,
s3Pf,S s1, s2, s3, s4Pf,S ... (builds
an EM and a UM tree)
BASIC P-trees If Ai Real or Binary and fi,j(x) ?
jth bit of xi ()Pfi,j ,1
?()Pi,jjb..0 are basic ()P-trees of Ai,
s1..sk If Ai Categorical and fi,a(x)1 if
xia, else 0 ()Pfi,a,1? ()Pi,aa?RAi are
basic ()P-trees of Ai Notes The UM masks
(e.g., of 2k,...,20Pi,j, with kroof(log2R ),
form a (binary) tree. Whenever the EM bit is 1,
that entire subtree can be eliminated (since it
represents a pure0 segment), then a 0-node at
level-k (lowest level level-0) with no sub-tree
indicates a 2k-run of zeros. In this
construction, the UM tree is redundant. We call
these EM trees the basic binary P-trees. The
next slide shows a top-down (easy to understand)
construction of and the following slide is a
(much more efficient) bottom up construction of
the same. We have suppressed the leafsize prefix.
7
Vertical basic binary Predicate-tree (P-tree)
vertically partition table compress each
vertical bit slice into a basic binary P-tree as
follows
A data table, R(A1..An), containing horizontal
structures (records) is
processed vertically (vertical scans)
then process using multi-operand logical ANDs.
R11 0 0 0 0 1 0 1 1
The basic binary P-tree, P1,1, for R11 is built
top-down by record truth of predicate pure1
recursively on halves, until purity.
But it is pure (pure0) so this branch ends
8
R11 0 0 0 0 1 0 1 1
Top-down construction of basic binary P-trees is
good for understanding, but bottom-up is more
efficient.
Bottom-up construction of P11 is done using
in-order tree traversal and the collapsing of
pure siblings, as follow
R11 R12 R13 R21 R22 R23 R31 R32 R33 R41 R42
R43
P11

0 1 0 1 1 1 1 1 0 0 0 1 0 1 1
1 1 1 1 1 0 0 0 0 0 1 0 1 1
0 1 0 1 0 0 1 0 1 0 1 1 1 1
0 1 1 1 1 1 0 1 0 1 0 0 0 1
1 0 0 0 1 0 0 1 0 0 0 1 1 0
1 1 1 1 0 0 0 0 0 1 1 0 0 1 1
1 0 0 0 0 0 1 1 0 0
0

9
Processing Efficiencies? (prefixed leaf-sizes
have been removed)
R(A1 A2 A3 A4)
2 7 6 1 6 7 6 0 2 7 5 1 2 7 5
7 5 2 1 4 2 2 1 5 7 0 1 4 7 0 1
4
21-level has the only 1-bit so the 1-count
121 2
10
A useful functional TV(a) ?x?R(x-a)o(x-a)
If we use d for a index variable over the
dimensions,
?x?R?d1..n(xd2 - 2adxd
ad2)
i,j,k bit slices indexes
Note that the first term does not depend upon a.
Thus, the derived attribute, TV-TV(?) (eliminate
1st term) is much simpler to compute and has
identical contours (just lowers the graph by
TV(?) ). We also find it useful to post-compose
a log to reduce the number of bit slices. The
resulting functional is called the
High-Dimension-ready Total Variation or HDTV(a).
11
?dadad )
TV(a) ?x,d,i,j 2ij xdixdj
R ( -2?dad?d
From equation 7, f(a)TV(a)-TV(?)
?d(adad- ?d?d) )
R ( -2?d(ad?d-?d?d)
R a-?2 so f(?)0
The length of g(a) depends only on the length of
a-?, so isobars are hyper-circles centered at ?
The graph of g is a log-shaped hyper-funnel

go inward and outward along a-? by
? to the points inner point, b?(1-?/a-?)(a-?)
and outer point, c?-(1?/a-?)(a-?).
For an ?-contour ring (radius ? about a)
Then take g(b) and g(c) as lower and upper
endpoints of a vertical interval. Then we use
EIN formulas on that interval to get a mask
P-tree for the ?-contour (which is a well-pruned
superset of the ?-neighborhood of a)
12

use circumscribing A?d-contour (Note A?d is not
a derived attribute at all, but just Ad, so we
already have its basic P-trees).
If the HDTV circumscribing contour of a is still
too populous,
• As pre-processing, calculate basic P-trees for
the HDTV derived attribute
• (or another hypercircular contour derived
attribute).
• To classify a
• 1. Calculate b and c (Depend on a, ?)
• 2. Form mask P-tree for training pts with
HDTV-values?HDTV(b),HDTV(c)
• 3. User that P-tree to prune out the candidate
NNS.
• If the count of candidates is small, proceed to
scan and assign class votes using Gaussian vote
function, else prune further using a dimension
projections).

HDTV(x)
HDTV(c)
We can also note that HDTV can be further
simplified (retaining same contours) using
h(a)a-?. Since we create the derived
attribute by scanning the training set, why not
just use this very simple function? Others leap
to mind, e.g., hb(a)a-b
HDTV(b)
x1
contour of dimension projection f(a)a1
b
c
x2
13
Graphs of functionals with hyper-circular contours
14
Angular Variation functionals e.g., AV(a) ? (
1/a ) ?x?R xoa d is an index over the
dimensions,
(1/a)?x?R?d1..nxdad
(1/a)?d(?xxdad) factor out ad
COS?(a) ? AV(a)/(?R) ?oa/(?a)
cos(?a?)
COS? (and AV) has hyper-conic isobars center on ?
COS? and AV have ?-contour(a) the space
between two hyper-cones center on ? which just
circumscribes the Euclidean ?-hyperdisk at a.
Intersection (in pink) with HDTV ?-contour.
Graphs of functionals with hyper-conic
contours E.g., COSb(a) for any vector, b
15
Given a training set, R(A1..An,C) the class
functional for class attribute value, c?C, is
functional, fR?C given by f(x)x.C. The class
isobar for class attribute value, c?C, f-1(c), is
then just the class itself. Since the class
functional is one of the dimension projection
functionals, it is not a new derived attribute
functional (basic P-trees already exist.)
16
A principle A job is not done until the
Mathematics is completed. The Mathematics of a
research job includes 1. Proving claims
(theorems, performance evaluation, simulation,
etc.), 2. simplification (everything is simple
once fully understood), 3. generalization (to
the widest possible application scope), and 4.
insight (what are the main issues and underlying
mega-truths (with full drill down)).
Therefore, we need to ask the following
questions at this point
Should we use the vector of medians (the only
good choice of middle point in mulidimensional
space, since the point closest to the mean
definition is influenced by skewness, like the
mean). We will denote the vector of medians
as ? h?(a)a-? is an important functional
(better than h?(a)a-??) If we compute the
median of an even number of values as the
count-weighted average of the middle two values,
then in binary columns, ? and ? coincide. (so if
µ and ? are far apart, that tells us there is
high skew in the data (and the coordinates where
they differ are the columns where the skew is
found).
17
Additional Mathematics to enjoy
What about the vector of standard deviations, ??
(computable with P-trees!) Do we have an
improvement of BIRCH here? - generating similar
comprehensive statistical measures, but much
faster and more focused?) We can do the same for
any rank statistic (or order statistic), e.g.,
vector of 1st or 3rd quartiles, Q1 or Q3 the
vector of kth rank values (kth ordinal
values). If we preprocessed to get the basic
P-trees of ?, and each mixed quartile vector
(e.g., in 2-D add 5 new derived attributes ?,
Q1,1, Q1,2, Q2,1, Q2,2 where Qi,j is the ith
quartile of the jth column), what does this tell
us (e.g., what can we conclude about the location
of core clusters? Maybe all we need is the basic
P-trees of the column quartiles, Q1..Qn ?)
• L? ordinal disks
• disk?(C,k) x xd is one of the k-Nearest
Neighbors of ad ? d.
• skin?(C,k), closed skin?(C,k) and ring?(C,k) are
defined as above.

Are they easy P-tree computations? Do they
offer advantages? When? What? Why? E.g.,
do they automatically normalize for us?
18
Dataset
• KDDCUP-99 Dataset (Network Intrusion Dataset)
• 4.8 millions records, 32 numerical attributes
• 6 classes, each contains gt10,000 records
• Class distribution
• Testing set 120 records, 20 per class
• 4 synthetic datasets (randomly generated)
• 10,000 records (SS-I)
• 100,000 records (SS-II)
• 1,000,000 records (SS-III)
• 2,000,000 records (SS-IV)

Normal 972,780
IP sweep 12,481
Neptune 1,072,017
Port sweep 10,413
Satan 15,892
Smurf 2,807,886
19
Speed and Scalability
• Speed (Scalability) Comparison (k5, hs25)

Algorithm x 1000 cardinality x 1000 cardinality x 1000 cardinality x 1000 cardinality x 1000 cardinality
Algorithm 10 100 1000 2000 4891
SMART-TV 0.14 0.33 2.01 3.88 9.27
P-KNN 0.89 1.06 3.94 12.44 30.79
KNN 0.39 2.34 23.47 49.28 NA
Running Time Against Varying Cardinality
100
Machine Intel Pentium 4 CPU 2.6 GHz, 3.8GB RAM,
running Red Hat Linux Note these evaluations
were done when we were still sorting the derived
TV attribute and before we used Gaussian vote
weighting. Therefore both speed and accuracy of
SMART-TV have improved markedly!
SMART-TV
90
PKNN
KNN
80
70
60
50
Time in Seconds
40
30
20
10
0
1000
2000
3000
4891
Training Set Cardinality (x1000)
20
Dataset (Cont.)
• OPTICS dataset
• 8,000 points, 8 classes (CL-1, CL-2,,CL-8)
• 2 numerical attributes
• Training set 7,920 points
• Testing set 80 points, 10 per class

21
Dataset (Cont.)
• IRIS dataset
• 150 samples
• 3 classes (iris-setosa, iris-versicolor, and
iris-virginica)
• 4 numerical attributes
• Training set 120 samples
• Testing set 30 samples, 10 per class

22
Overall Accuracy
• Overall Classification Accuracy Comparison

Datasets SMART-TV PKNN KNN
IRIS 0.97 0.71 0.97
OPTICS 0.96 0.99 0.97
SS-I 0.96 0.72 0.89
SS-II 0.92 0.91 0.97
SS-III 0.94 0.91 0.96
SS-IV 0.92 0.91 0.97
NI 0.93 0.91 NA
23
More Mathematics to enjoy
As you probably know, Taufik used a heap process
to get the k nearest neighbors of unclassified
samples (requiring one scan through the
well-pruned nearest neighbor candidate
set). This means that Taufik did not use the
closed kNN set, so accuracy will be the same as
horizontal kNN (single scan, non-closed)
Actually, accuracy varies slightly depending on
the kth NN picked from the ties). Taufik is
planning to leave the thesis that way and explain
why he did it that way (over-fairness) -) A
great learning experience with respect to using
DataMIME and a great opportunity for thesis
exists here - namely showing that when one uses
closed kNN in SMART-TV, not only do we
get a tremendously scalable algorithm, but also a
much more accurate result (even
slightly faster since no heaping to do). A
project Re-run Taufik's performance measurements
(just for SMART-TV) using a final scan of the
pruned candidate Nearest Neighbor Set. Let all
candidates vote using a Gaussian vote drop-off
function as
24
More Mathematics to enjoy
Meegeum has claimed the study of how much the
above improves accuracy in various settings (and
how various parameterization of g(x) (ala
statistics) affect it)? More enjoyment! If
there is a example data set where accuracy goes
down when using the Gaussian and closed NNS, that
proves the data set is noise at that point?
(i.e., ? no continuity relationship between
features and classes at a). This leads to an
interesting theory regarding the continuity of a
training set. Everyone assume the training set
is what you work with and you assume
continuity! In fact, Cancer studies forge ahead
with classification even when pgtgtn, that is there
are just too few feature tuples for continuity
to even makes good sense! So now we have a
method of identifying those regions of the
training space where there is a discontinuity
in the feature-to-class relationship, FC. That
will be valuable (e.g., in Bioinformatics). Has
this been studied? (I think, everyone just goes
ahead and classifies based on continuity and
lives with the result!
25
More enjoyment (continued)
I claim, when the class label is real, then with
a properly chosen Gaussian vote-weight function
(chosen by domain experts) and with a good method
of testing the classifier, if SMART-TV
miss-classifies a test point, then it is not a
miss-classification at all, but there is a
discontinuity in FC there (between feature and
class)! In other words, that proves the training
set is wrong there and should not be used. I am
claiming (do you back me up, Dr. Abidin?)
SMART-TV, properly tuned, DEFINES CORRECT! It
will be fun to see how far we can go with this
point of view. Be warned - it will cause quite
a stir! Thoughts 1. Choose a vote drop-off
Gaussian carefully (with domain knowledge in
play) and designates it as "right". What could be
more right? - if you agree that classification
has to be based on FC continuity. 2. Analyze
(very carefully) SMART-TV vote histograms for ?1
lt ?2 lt ... lt ?h If all are inconclusive then
the Feature-to-Class function (FC) is
discontinuous at a and classification SHOULD NOT
BE ATTEMPTED USING THAT TRAINING SET! (This may
get us into Krigging). If the histograms get
more and more conclusive as the radius increases,
then possibly one would want to rely on the outer
ring votes, but one should also report that there
is class noise at a! 3. We can get into tuning
the Modified Gaussian, MG, by region. Determine
the subregion where MC gives conclusive
histograms. Then for each inconclusive training
point, examine modifications (other parameters or
even other dropoff functions) until you find one
that is conclusive. Determine the full region
where that one is conclusive ...
26
More enjoyment (continued)
CAUTION! Many important Class Label Attributes
(CLAs) are real (e.g., level of ill intent in
homeland security data mining, level of ill
intent in Network Intrusion analysis, probability
of cancer in a cancer tissue microarray dataset),
but many important Class Label Attributes are
categorical (e.g., bioinformatic anotation,
Phenotype prediction, etc.). When the Class
label is categorical, the distance on the CLA
becomes the characteristic distance function
(distance0 iff the 2 categories are different).
Continuity at a becomes ??gt0 ?d(x,a)lt? ?
f(x)f(a). Possibly boundary determination of
the training set classes is most important in
case? Is that why SVM works so well in those
situations? Still, For there to be continuity at
a, it must be the case that some NNS of a maps to
f(a). However, if the (CLA) is real Has anyone
every seen analysis of "what is the best
definition of correct" done (do statistician do
this?). Maybe we need a good statistician to
take a look, but let's pursue it anyway so we
know what we can claim. Step 1 re-run Taufik's
SMART-TV with the Modified Gaussian, MG(x), and
closed kNN. We should also take several standard
UCI ML classification datasets, randomize classes
in some particular isotropic neighborhood (so
that we know where there is definitely an FC
discontinuity) Then show (using matlab?) that
SVM fails to detect that discontinuity (i.e., SVM
gives a definitive class to it without the
ability to detect the fact that it doesn't
deserve one? or do the Lagrange multipliers do
that for them?) and then show that we can detect
that situation. Does any other author do
this? Other fun ideas?
27
Classifying with multi-relational
(heterogeneous) training data
Classify on a foreign key table (S) when some of
the features need to come from the primary key
table (R)?
R(A0 A1 A2)
S(B0 B1 B2 B3)
010 111 110 001 011 111 110 000 010 110
101 001 010 111 101 111 101 010 001
100 010 010 001 101 111 000 001 100 111
000 001 100
001 11 0 011 10 1 100 01 1 101
01 0 110 11 0 111 00 0
S11 S12 S13 S21 S22 S23 S31 S32 S33 S41 S42
S43
To data mine this PK multi-relation, (R.A0 is
ordered ascending primary key and S.B2 is the
foreign key), scan S building (basic P-trees
for) the derived attributes Bn1..Bnm (here
B4,B5) from A1..Am using the bottom up approach
(next slide)? Note Once the derived basic
P-trees are built, what if a tuple is added to S?
If it has a new B2-value then a new tuples must
have been added to R also (with that value in
A0). Then all basic P-trees must be extended
(including the derived).

0 1 0 1 1 1 1 1 0 0 0 1 0 1 1
1 1 1 1 1 0 0 0 0 0 1 0 1 1
0 1 0 1 0 0 1 0 1 0 1 1 1 1
0 1 1 1 1 1 0 1 0 1 0 0 0 1
1 0 0 0 1 0 0 1 0 0 0 1 1 0
1 1 1 1 0 0 0 0 0 1 1 0 0 1 1
1 0 0 0 0 0 1 1 0 0
28
R(B2 A1 A2)
S(B0 B1 B2 B3)
010 111 110 001 011 111 110 000 010 110
101 001 010 111 101 111 101 010 001
100 010 010 001 101 111 000 001 100 111
000 001 100
001 11 0 011 10 1 100 01 1 101
01 0 110 11 0 111 00 0
S.B4,1
S.B4,2
S.B5
0
1
0

The cost is the same as an indexed nested loop
join (reasonable to assume there is a primary
index on R). When an insert is made to R,
nothing has to change. When an insert is made to
S, the P-tree structure is expanded and filled
using the values in that insert plus the
R-attribute values of the new S.B2 value (This is
one index lookup. The S.B2 value must exists in
R.A0 by referential integrity). Finally, if we
are using, e.g. 4Pi,j P-trees instead of the
(4,2,1)Pi,j P-trees shown here, it's the
same The basic P-tree fanout is /\ , the left
leaf is filled by the first 4 values, the right
leaf is filled with the last 4.
29
If we are using, e.g. 4Pi,j P-trees instead of
the (4,2,1)Pi,j P-trees shown here, it's the
same The basic P-tree fanout is /\ , the left
leaf is filled by the first 4 values, the right
leaf is filled with the last 4.
R(B2 A1 A2)
S(B0 B1 B2 B3)
010 111 110 001 011 111 110 000 010 110
101 001 010 111 101 111 101 010 001
100 010 010 001 101 111 000 001 100 111
000 001 100
001 11 0 011 10 1 100 01 1 101
01 0 110 11 0 111 00 0

1 1
1 1
0 0
1 1 1 1
1 1 1 1
0 0 0 0

0 0
1 1
0 0
or
30
VertiGO (Vertical Gene Ontology)
The GO is a data structure which needs to be
mined together with various valuable
bioinformatic data sets. Biologists waste time
searching for all available information about
each small area of research. This is hampered
further by variations in terminology in common
usage at any given time, and that inhibit
effective searching by computers as well as
people. E.g., In a search for new targets for
antibiotics, you want all gene products involved
in bacterial protein synthesis, that have
significantly different sequence or structure
from those in humans. If one DB says these
molecules are involved in 'translation' and
another uses 'protein synthesis', it is
difficult for you - and even harder for a
computer - to find functionally equivalent
terms. GO is an effort to address the need for
consistent descriptions of gene products in
different DBs. The project began in 1988 as a
collaboration between three model organism
databases FlyBase (Drosophila), Saccharomyces
Genome Database (SGD) Mouse Genome Database
(MGD). Since then, the GO Consortium has grown
to include several of the world's major
repositories for plant, animal and microbial
genomes. See the GO web page for a full list of
member orgs.
31
VertiGO (Vertical Gene Ontology)
The GO is a DAG which needs to be mined in
conjunction with the Gene Table (one tuple for
each gene with feature attributes). The DAG
links are IS-A or PART-OF links. (Description
follows from the GO website). If we take the
simplified view that the GO assigns annotations
of types ( Biological Process (BP) Molecular
Function (MF) Cellular Component (CC)) to
genes, with qualifiers ( "contributes to",
"co-localizes with", "not" ) and evidence
codes IDAInferredfromDirectAssay
IGIInferredfromGeneticInteraction,
IMPInferredfromMutantPhenotype
IPIInferredfromPhysicalInteraction,
TASTraceableAuthorStatement IEPInferredfromExpr
essionPattern, RCAInferredfromReviewedComputation
alAnalysis, ICInferredbyCurator
IEAInferredbyElectronicAnnotation
ISSInferredfromSequence/StructuralSimilarity,
NASNontraceableAuthorStatement,
NDNoBiologicalDataAvailable, NRNotRecorded Solut
ion-1 For each annotation (term or GOID) have a
2-bit type code column GOIDType BP11 MF10
CC00 and a 2-bit qualifier code column
GOIDQualifier with contributesto11,
co-localizeswith10 and not00 and a
4-bit evidence code column GOIDEvidence e,g,
IDA1111, IGI-1110, IMP1101, IPI1100, TAS1011,
IEP1010, ISS1001, RCA1000, IC0111, IEA0110,
NAS0100, ND0010, NR0001 (putting DAG
structure in schema catalog). (Increases width
by 8-bits GOIDs to losslessly incorporate the
GO info). Solution-2 BP, MF and CC DAGs are
disjoint (share no GOIDs? true?), an alternative
solution is Use a 4-bit evidencecode/qualif
ier column, GOIDECQ For evidence codes IDA1111
IGI-1110 IMP1101 IPI1100 TAS1011 IEP1010
ISS1001 RCA1000 IC0111 IEA0110 NAS0101
ND0100 NR0011. Qualifiers 0010contributesto
0001colocalizeswith 0000not (width increases
4-bitsGOID lossless GO). Solution-3 bitmap all
13 evidencecodes and all 3 qualifiers (adds 16
bit map per GO term). Keep in mind that genes
are assumed to be inherited up the DAGs but are
only listed at the lowest level to which they
apply. This will keep the bitmaps sparse. If a
GO term has no attached genes, it need not be
included (many such?). It will be in the schema
with its DAG links, and will be assumed to
inherit all downstream genes, but it will not
generate 16 bit columns in Gene Table). Is the
not qualifier the complement of the term
bitmap? Solution-4 bitmap all 19 attributes
(type, qualifiers, evidence codes)
32
GO has 3 structured, controlled vocabularies
(ontologies) describing gene products (the RNA or
protein resulting after transcription) by their
species-independent, associated
biological processes (BP), cellular components
(CC) molecular functions (MF). There are three
separate aspects to this effort The GO
consortium 1. writes and maintains the
ontologies themselves 2. makes associations
between the ontologies and genes / gene products
in the collaborating DBs, 3. develops tools that
facilitate the creation, maintainence and use of
ontologies. The use of GO terms by several
collaborating databases facilitates uniform
queries across them. The controlled vocabularies
are structured so that you can query them at
different levels e.g., 1. use GO to find all
gene products in the mouse genome that are
involved in signal transduction, 2. zoom in on
all the receptor tyrosine kinases. This
structure also allows annotators to assign
properties to gene products at different levels,
depending on how much is known about a gene
product.
GO is not a database of gene sequences or a
catalog of gene products
GO describes how gene products behave in a
cellular context. GO is not a way to unify
biological databases (i.e. GO is not a 'federated
solution'). Sharing vocabulary is a step towards
unification, but is not sufficient. Reasons
include Knowledge changes and updates lag
behind.
33
Curators evaluate data differently (e.g., agree
to use the word 'kinase', but not to support this
by stating how and why we use 'kinase', and
consistently to apply it. Only in this way can we
hope to compare gene products and determine
whether they are related. GO does not attempt to
describe every aspect of biology. For example,
domain structure, 3D structure, evolution and
expression are not described by GO. GO is not a
dictated standard, mandating nomenclature across
databases. Groups participate because of
self-interest, and cooperate to arrive at a
consensus. The 3 organizing GO principles
molecular function, biological process, cellular
component. A gene product has one or more
molecular functions and is used in one or more
biological processes it might be associated with
one or more cellular components. E.g., the gene
product cytochrome c can be described by the
molecular function term oxidoreductase activity,
the biological process terms oxidative
phosphorylation and induction of cell death, and
the cellular component terms mitochondrial
matrix, mitochondrial inner membrane.
Molecular function (organizing principle of GO)
describes e.g., catalytic/binding activities, at
molecular level GO molecular function terms
represent activities rather than the entities
(molecules / complexes) that perform actions, and
do not specify where or when, or in what context,
the action takes place. Molecular functions
correspond to activities that can be performed by
individual gene products, but some activities are
performed by assembled complexes of gene
products. Examples of broad functional terms are
catalytic activity, transporter activity, or
binding Examples of narrower functional terms
are adenylate cyclase activity or Toll receptor
binding. It is easy to confuse a gene product
with its molecular function, and for thus many GO
molecular functions are appended with the word
"activity". The documentation on gene products
explains this confusion in more depth.
34
A Biological Process is series of events
accomplished by 1 or more ordered assemblies of
molecular fctns. Examples of broad biological
process terms cellular physiological process or
signal transduction. Examples of more specific
terms are pyrimidine metabolism or
alpha-glucoside transport. It can be difficult to
distinguish between a biological process and a
molecular function, but the general rule is that
a process must have more than one distinct
steps. A biological process is not equivalent to
a pathway. We are specifically not capturing or
trying to represent any of the dynamics or
dependencies that would be required to describe a
pathway. A cellular component is just that, a
component of a cell but with the proviso that it
is part of some larger object, which may be an
anatomical structure (e.g. rough endoplasmic
reticulum or nucleus) or a gene product group
(e.g. ribosome, proteasome or a protein dimer).
What does the Ontology look like? GO terms are
organized in structures called directed acyclic
graphs (DAGs), which differ from hierarchies in
that a child (more specialized term) can have
many parent (less specialized term). For
example, the biological process term hexose
biosynthesis has two parents, hexose metabolism
and monosaccharide biosynthesis. This is because
biosynthesis is a subtype of metabolism, and a
hexose is a type of monosaccharide. When any
gene involved in hexose biosynthesis is annotated
to this term, it is automatically annotated to
both hexose metabolism and monosaccharide
biosynthesis, because every GO term must obey the
true path rule if the child term describes the
gene product, then all its parent terms must also
apply to that gene product.
35
It is easy to confuse a gene product and its
molecular function, because very often these are
described in exactly the same words. For example,
'alcohol dehydrogenase' can describe what you can
put in an Eppendorf tube (the gene product) or it
can describe the function of this stuff. There
is, however, a formal difference a single gene
product might have several molecular functions,
and many gene products can share a single
molecular function, e.g., there are many gene
products that have the function 'alcohol
dehydrogenase'. Some, but by no means all, of
these are encoded by genes with the name alcohol
dehydrogenase. A particular gene product might
have both the functions 'alcohol dehydrogenase'
and 'acetaldehyde dismutase', and perhaps other
functions as well. It's important to grasp that,
whenever we use terms such as alcohol
dehydrogenase activity in GO, we mean the
function, not the entity for this reason, most
GO molecular function terms are appended with the
word 'activity'. Many gene products associate
into entities that function as complexes, or
'gene product groups', which often include small
molecules. They range in complexity from the
relatively simple (for example, hemoglobin
contains the gene products alpha-globin and
beta-globin, and the small molecule heme) to
complex assemblies of numerous different gene
products, e.g., the ribosome. At present, small
molecules are not represented in GO. In the
future, we might be able to create cross products
by linking GO to existing databases of small
molecules such as Klotho , LIGAND
36
How do terms get associated with gene
products? Collaborating databases annotate their
gene products (or genes) with GO terms, providing
references and indicating what kind of evidence
is available to support the annotations. More
info in GO Annotation Guide. If you browse any
of the contributing databases, you'll find that
each gene or gene product has a list of
associated GO terms. Each database also publishes
a table of these associations, and these are
freely available from the GO ftp site. You can
also browse the ontologies using a range of
web-based browsers. A full list of these, and
other tools for analyzing gene function using GO,
is available on the GO Tools page . In
addition, the GO consortium has prepared GO
slims, 'slimmed down' versions of the ontologies
that allow you to annotate genomes or sets of
gene products to gain a high-level view of gene
functions. Using GO slims you can, for example,
work out what proportion of a genome is involved
in signal transduction, biosynthesis or
reproduction. See the GO Slim Guide for more
information.
All GO data is free. Download the ontology data
in a number of different formats, including XML
and mySQL, from the GO Downloads page (more info
on syntax of these formats, GO File Format
Guide. If you need lists of the genes or gene
products that have been associated with a
particular GO term, the Current Annotations table
tracks the number of annotations and provides
links to gene association files for each of
collaborating DBs is available. GO allows us to
annotate genes and their products with a limited
set of attributes. e.g., GO does not allow us to
describe genes in terms of which cells or tissues
they're expressed in, which developmental stages
they're expressed at, or their involvement in
disease. It is not necessary for GO to this
since other ontologies are doing it. The GO
consortium supports the development of other
ontologies and makes its tools for editing and
curating ontologies available. A list of freely
available ontologies that are relevant to
genomics and proteomics and are structured
similarly to GO can be found at the Open
Biomedical Ontologies website. A larger list,
which includes the ontologies listed at OBO and
also other controlled vocabularies that do not
fulfil the OBO criteria is available at the
Ontology Working Group page of the Microarray
Gene Expression Data Society (MGED).
37
Cross-products The existence of several
ontologies will also allow us to create
'cross-products' that maximize the utility of
each ontology while avoiding redundancy. For
example, by combining the developmental terms in
the GO process ontology with a second ontology
that describes Drosophila anatomical structures,
we could create an ontology of fly
development. We could repeat this process for
other organisms without having to clutter up GO
with large numbers of species-specific terms.
Similarly, we could create an ontology of
biosynthetic pathways by combining biosynthesis
terms in the GO process ontology with a chemical
ontology. Mappings to other classification
systems GO is not the only attempt to build
structured controlled vocabularies for genome
annotation. Nor is it the only such series of
catalogs in current use. We have attempted to
make translation tables between these catalogs
and GO. We caution that these mappings are
neither complete nor exact they are to be used
as a guide. One reason for this is absence of
definitions from many of the other catalogs and
of a complete set of definitions in GO itself.
More information on the syntax of these mappings
can be found in the GO File Format Guide.
Contributing to GO The GO project is constantly
evolving, and we welcome feedback from all users.
If you need a new term or definition, or would
like to suggest that we reorganize a section of
one of the ontologies, please do so through our
online request-tracking system, which is hosted
by SourceForge.net.
What is a GO term? The purpose of GO is to
define particular attributes of gene products. A
term is simply the text string used to describe
an entry in GO, e.g. cell, fibroblast growth
factor receptor binding or signal transduction.
A node refers to a term and all its children. GO
does not contain the following Gene products
e.g. cytochrome c is not in GO attributes of it,
e.g., oxidoreductase activity, are. Processes,
functions or components that are unique to
mutants or diseases e.g. oncogenesis is not a
valid GO term because causing cancer is not the
normal function of any gene. Attributes of
sequence such as intron/exon parameters these
are not attributes of gene products and will be
described in a separate sequence ontology (see
OBO web site for more information). Protein
domains or structural features. Protein-protein
interactions.
38
Conventions when adding a term These stylistic
points should be applied to all aspects of the
ontologies. Spelling conventions Where there
are differences in accepted spelling between
English and US, use US form, e.g. polymerizing,
signaling, rather than polymerising, signalling.
A dictionary of 'words' used in GO terms at
GODict.DAT Abbreviations Avoid abbreviations
unless they're self-explanatory. Use full element
names, not symbols. Use hydrogen for H. Use
copper and zinc rather than Cu and Zn. Use
copper(II), copper(III), etc., rather than
cuprous, cupric, etc. For biomolecules, spell out
the term in full wherever practical use
fibroblast growth factor, not FGF. Greek
symbols Spell out Greek symbols in full e.g.
alpha, beta, gamma. Case GO terms are all
lower case except where demanded by context, e.g.
DNA, not dna. Singular/plural Use singula,
except where a term is only used in plural (eg
caveolae). Be descriptive Be reasonably
descriptive, even at the risk of verbal
redundancy. Remember, DBs that refer to GO terms
might list only the finest-level terms associated
with a particular gene product. If the parent is
aromatic amino acid family biosynthesis, then
child should be aromatic amino acid family
biosynthesis, anthranilate pathway, not
anthranilate pathway. Anatomical qualifiers Do
not use anatomical qualifiers in the cellular
process and molecular function ontologies. For
example, GO has the molecular function term
DNA-directed DNA polymerase activity but neither
nuclear DNA polymerase nor mitochondrial DNA
polymerase. These terms with anatomical
qualifiers are not necessary because annotators
can use the cellular component ontology to
attribute location to gene products,
independently of process or fctn.
Synonyms When several words or phrases that
could be used as the term name, one form will be
chosen as term name whilst the other possible
names are added as synonyms. Despite the name,
GO synonyms are not always 'synonymous' in the
strictest sense of the word, as they do not
always mean exactly the same as the term they are
attached to. Instead, a GO synonym may be broader
or narrower than the term string it may be a
related phrase it may be alternative wording,
spelling or use a different system of
nomenclature or it may be a true synonym. This
flexibility allows GO synonyms to serve as
valuable search aids, as well as being useful for
apps such as text mining and semantic matching.
Having a single, broad relationship between a GO
term and its synonyms is adequate for most search
purposes, but for other applications such as
semantic matching, the inclusion of a more formal
relationship set is valuable. Thus, GO records a
relationship type for each synonym, stored in OBO
format flat file. Synonym types The synonym
relationship types are term is an exact
synonym (ornithine cycle is an exact synonym of
urea cycle) terms are related (cytochrome bc1
complex is a related to ubiquinol-cytochrome-c
reductase activity) synonym is broader than the
term name (cell division is a broad synonym of
cytokinesis) synonym is narrower or more precise
(pyrimidine-dimer repair by photolyase is a
narrow synonym of photoreactive repair) synonym
is related to, but not exact, broader or narrower
(virulence has synonym type of other related to
term pathogenesis)
39
These types form a loose hierarchy related
i exact synonym i broad synonym i
narrow synonym i other related synonym
The default relationship is related to, as all
synonyms are in some way related to the term
name, but more specific relationships are
assigned where possible. The synonym type other
related is used where the relationship between a
term and its synonym is NOT exact, narrower or
broader. In some cases, broader and narrower
synonyms are created in the place of new parent
or child terms because some synonym strings may
not be valid GO terms but may still be useful for
search purposes. This may be because the synonym
is the name of a gene product e.g.
ubiquitin-protein ligase activity has the
narrower synonym E3, as E3 is a specific gene
product with ubiquitin-protein ligase activity.
Adding synonyms When you add a synonym using
DAG-Edit, choose a type from the pull-down
selector (see the DAG-Edit user guide for more
information). DAG-Edit will incorporate the
synonym type into the OBO format flat file when
you save. The default synonym type is the
broadest, 'synonym' (equivalent to 'related'
above). Number of synonyms for a term is not
limited, and the same text string can be used
for more than 1 GO term. Add synonyms if you
edit a term name but the old name is still a
valid synonym for example, if you change
respiration to cellular respiration, keep
respiration as a synonym. This helps other users
find familiar terms. Add synonyms if the term
has (or contains) a commonly used abbreviation.
For example, FGF binding could be used as a
synonym for fibroblast growth factor binding. Do
not add a synonym if the only difference is case
(e.g. start vs. START). Synonyms, like term
names, are all lower case except where demanded
by context (e.g. DNA, not dna).
Rules for Synonyms Acronyms are exactly
synonymous with full name (if acronym is not used
in any other sense elsewhere) 'Jargon' type
phrases are exactly synonymous w full name (if
phrase is not used in any other sense
elsewhere) proton is exactly synonymous with
hydrogen in most senses EXCEPT where hydrogen
means H2 (i.e. gas) include implicit information
when making decision take into account which
ontology the term is in - e.g. an entry term that
ends in 'factor' is not synonymous with a
molecular function. ligand is NOT exactly
synonymous with binding (ligand is an entity,
binding an action) XXX receptor ligand is NOT
exactly synonymous with XXX (1 potential ligands
so XXX receptor ligand broader than XXX XXX
complex is NOT exactly synonymous with XXX (XXX
is ambiguous - could describe activity of XXX)
porter and transporter are NOT exactly
synonymous (transporter is broader)
symporter/antiporter and transporter are NOT
exactly synonymous (transporter is broader)
40
General database cross references (general
dbxrefs) should be used whenever a GO term has an
identical meaning to an object in another
database. Some ex. of common general dbxrefs in
GO Ontology DB Sample dbxref Fctn Enzyme
Commission EC3.5.1.6 Transport Protein Database
TC2.A.29.10.1 Biocatalysis/Biodegradation DB
UM-BBD_enzymeIDe0310 Biocatalysis/Biodegradation
DB UM-BBD_pathwayIDdcb MetaCyc Metabolic Pathway
DB MetaCycXXXX-RXN Process MetaCyc Metabolic
Pathway DB MetaCyc2ASDEG-PWY Component None
The GO.xrf_abbs file is maintained by the BioMOBY
project, so to make changes to the file, you need
to use their web form.
Understanding relationships in GO GO ontologies
are structured as a directed acyclic graph (DAG),
which means that a child (more specialized) term
can have multiple parents (less specialized
terms). This makes GO a powerful system to
describe biology, but creates some pitfalls for
curators Keeping the following guidelines in mind
should help you to avoid these problems. A child
term can have one of two different relationships
to its parent(s) is_a or part_of. The same term
can have different relationships to different
parents for example, the child 'GO term 3' may
be an is_a of parent 'GO term 1' and a part_of
parent, 'GO term 2' In GO, an is_a relationship
means that the term is a subclass of its parent.
For example, mitotic cell cycle is_a cell cycle,
not confused with an 'instance' which is a
specific example. E.g., clogs are a subclass or
is_a of shoes, while the shoes I have on my feet
now are an instance of shoes. GO, like most
ontologies, does not use instances. The is_a
relationship is transitive, which means that if
'GO term A' is a subclass of 'GO term B', and 'GO
term B' is an subclass of 'GO term C', 'GO term
A' is also a subclass of 'GO term C', E.g.,
Terminal N-glycosylation is a subclass of
terminal glycosylation. Terminal glycosylation
is a subclass of protein glycosylation. Terminal
N-glycosylation is a subclass of protein
glycosylation.
part_of in GO is more complex. There are 4 basic
levels of restriction for a part_of relationship
1st type has no restrictions - no inferences can
be made from the relationship between parent and
child other than that parent may have child as a
part, and the child may or may not be a part of
the parent.
41
2nd type, 'necessarily is_part', means that
wherever the child exists, it is as part of the
parent. To give a biological example,
replication fork is part_of chromosome, so
whenever replication fork occurs, it is as
part_of chromosome, but chromosome does not
necessarily have part replication fork. 3rd
type, 'necessarily has_part', is the exact
inverse of type two wherever the parent exists,
it has the child as a part, but the child is not
necessarily part of the parent. For example,
nucleus always has_part chromosome, but
chromosome isn't necessarily part_of nucleus.
4th type, is a combination of both two and
three, 'has_part' and 'is_part'. An example of
this is nuclear membrane is part_of nucleus. So
nucleus always has_part nuclear membrane, and
nuclear membrane is always part_of nucleus. The
part_of relationship used in GO is usually type
two, 'necessarily is_part'. Note that part_of
types 1 and 3 are not used in GO, as they would
violate the true path rule. Like is_a, part_of is
transitive, so that if 'GO term A' is part_of 'GO
term B', and 'GO term B' is part_of 'GO term C',
'GO term A' is part_of 'GO term C' E.g.,
Laminin-1 is part_of basal lamina. Basal
lamina is part_of basement membrane. Laminin-1
is part_of basement membrane. The ontology
editing tool DAG-Edit, from version 1.411 on,
allows you to specify the necessity of
relationships. The part_of relationship used in
GO, necessarily is_part, would correspond to
part_of, inverse necessarily true. For more
information, see the DAG-Edit user guide. For
info on how these relationships are represented
in the GO flat files, see the GO File Format
Guide. For technical info on the relationships
used in GO and OBO, see the OBO relationships
ontology.
The true path rule states that "the pathway from
a child term all the way up to its top-level
parent(s) must always be true". One of the
implications of this is that the type of part_of
relationship used in GO, outlined more fully in
the part_of relationship section above, is
restricted to those types where a child term must
always be part_of its parent. Often, annotating
a new gene product reveals relationships in an
ontology that break the true path rule, or
species specificity becomes a problem. In such
cases, the ontology must be restructured by
adding more nodes and connecting terms such that
any path upwards is true. When a term is added to
the ontology, the curator needs to add all of the
parents and children of the new term. This
becomes clear with an example consider how
chitin metabolism is represented in the process
ontology. Chitin metabolism is a part of cuticle
synthesis in fly and is also part of cell wall
organization in yeast. This was once represented
in process ontology as cuticle synthesis,
ichitin metabolism, cell wall biosynthesis,
ichitin metabolism, ---ichitin biosynthesis,
---ichitin catabolism
42
Illustration The problem with this organization
becomes apparent when one tries to annotate a
specific gene product from one species. A fly
chitin synthase could be annotated to chitin
biosynthesis, and appear in a query for genes
annotated to cell wall biosynthesis (and its
children), which makes no sense because flies
don't have cell walls. This is revised ontology
structure which ensures that the true path rule
is not broken chitin metabolism, ichitin
biosynthesis, ichitin catabolism,
icuticle chitin metabolism ---icuticle chitin
biosynthesis, ---icuticle chitin catabolism
icell wall chitin metabolism, ---icell wall
chitin biosynthesis, ---icell wall chitin
catabolism Illustration The parent chitin
metabolism now has the child terms cuticle chitin
metabolism and cell wall chitin metabolism, with
the appropriate catabolism and synthesis terms
beneath them. With this structure, all the
daughter terms can be followed up to chitin
metabolism, but cuticle chitin metabolism terms
do not trace back to cell wall terms, so all the
paths are true. In addition, gene products such
as chitin synthase can be annotated to nodes of
appropriate granularity in both yeast and flies,
and queries will yield the expected results.
Dependent ontology terms Some GO terms imply
presence of others. Examples from process
ontology include If either X biosynthesis or X
catabolism exists, then parent X metabolism must
also exist. If regulation of X exists, then the
process X must also exist. Potentially any
process in the ontology can be regulated. Note X
may refer to a phenotype (for example cell size
in regulation of cell size in these cases, X
should not be added to ontology. GO nodes must
avoid using species-specific defs. Nevertheless,
many functions, processes and components are not
common to all life forms. Our current convention
is to include any term that can apply to more
than one taxonomic class of organism. Within
ontologies, are cases where a word/phrase has
different meanings for organisms. E.g., embryonic
development in insects is very different from
embryonic development in mammals. Such terms are
distinguished from one another by their
definitions and by the sensu designation (sensu
means 'in the sense of'), as in the term
embryonic development (sensu Insecta). Nodes
should be divided into sensu sub-trees where the
children are or are likely to be different. Using
sensu designation in a term does not exclude that
term from being used to annotate species outside
that designation. e.g., a 'sensu Drosophila' term
might reasonably used to annotate a mosquito gene
product. A GO node should never be more
species-specific than any of its children. Child
nodes can be at the same level of species
specificity as the parent node(s), or more
specific. When adding more species-specific
nodes, curators should make sure that
non-species-specific parents exist (or add them
if necessary). E.g., take the process of
sporulation. This occurs in both bacteria and
fungi, but bacterial sporulation is quite a
different process to fungal sporulation, so we
therefore add two children to sporulation,
sporulation (sensu Bacteria) and sporulation
(sensu Fungi). If we now want to add a term to
represent the assembly of the spore wall in
fungi, we cannot just add spore wall assembly as
a direct child of sporulation (sensu Fungi) as
such a term could conceivably refer to the
assembly of spore walls in bacteria. Name child
term spore wall assembly (sensu Fungi) to ensure
that it is as species-specific as parent term.
43
References and Evidence Every annotation must be
attributed to a source, which may be a literature
reference, another database or a computational
analysis. The annotation must indicate what
kind of evidence is found in the cited source to
support the association between the gene product
and the GO term. A simple controlled vocabulary
is used to record evidence IMP inferred from
mutant phenotype IGI inferred
from genetic interaction ltdbgene_symbolallele_sy
mbolgt IPI inferred from physical interaction w
ltdbprotein_namegt ISS inferred from
sequence similarity with ltdatabasesequence_idgt
IDA inferred from direct assay
IEP inferred from expression pattern IEA
inferred from electronic annotation with
ltdatabaseidgt TAS traceable author
statement NAS non-traceable author statement
ND
no biological data available RCA inferred from
reviewed computational analysis
IC inferred by curator from ltGOidgt
Annotation File Format Collaborating databases
export to GO a tab delimited file, known
informally as a "gene association file" of links
between database objects and GO terms. Despite
jargon, the db object may represent a gene or a
gene product (transcript or protein). Columns in
file are described below, a table showing columns
in order, with examples, is available. The entry
in the DB_Object_ID field (see below) of the
association file is the identifier for the
database object, which may or may not correspond
exactly to what is described in a paper. For
example, a paper describing a protein may support
annotations to the gene encoding the protein
(gene ID in DB_Object_ID field) or annotations to
a protein object (protein ID in DB_Object_ID
field). The entry in the DB_Object_Symbol field
should be a symbol that means something to a
biologist, wherever possible (gene symbol, for
example). It is not an ID or an accession number
(the second column, DB_Object_ID, provides the
unique identifier), although IDs can be used in
DB_Object_Symbol if there is no more biologically
meaningful symbol available (e.g., when an
unnamed gene is annotated). The object type
(gene, transcript, protein, protein_structure, or
complex) listed in the DB_Object_Type field MUST
match the database entry identified by
DB_Objec
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