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Antimicrobial Coatings

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Title: Antimicrobial Coatings


1
Antimicrobial Coatings
  • By Nikki Buck and
  • David Brink-Roby
  • Mentors Danielle Leiske and Alia Mulder

2
Background Information
  • Background
  • To compare the effectiveness of antimicrobial
    proteins and other inhibiting bacterial growth on
    biomedical devices.
  • What is bioengineering?
  • Applies engineering approaches to problems
    dealing with biological systems.
  • How does this apply to life?
  • Bacteria grows on tracheal tubes.
  • Antimicrobial coatings are needed to prevent
    infection.

3
Equipment and Materials
  • Agar
  • A gelatin compound used to stimulate the growth
    of bacteria.
  • Pediococcus
  • A gram positive bacteria grown in MRS agar.
  • E. Coli
  • A gram negative bacteria found naturally in the
    human body that causes stomach sickness.
  • Caliper
  • A tool used to measure the thickness of a solid.
  • Sterilization
  • Process of disinfecting a surface.
  • Stir Plates/ Stir Bars
  • A machine that uses magnets to stir a solution.
  • Tracheal Tube
  • A medical tube used in hospitals to clean out the
    air passageways of the trachea.
  • Autoclave
  • A machine used for sterilization.
  • Gram Positive
  • Bacterial with thicker cell walls.
  • Gram Negative

4
Procedure
  • Experiment 1
  • Make plates of MRS and McConkey Agar
  • Cut equal sized pieces of sterile tracheal tubing
  • Soak pieces of tubing in antimicrobial solutions
  • Rinse tubing in phosphate buffer
  • Place tubing in agar plates
  • Incubate the plates overnight
  • Measure and record the diameter of kill zones
    around each piece of tubing
  • Experiment 2
  • Repeat previous steps but air-dry tracheal tubing
    after rinsing in phosphate buffer

5
Antimicrobial Coatings
  • SDS- has a negatively charged head, hydrophobic
    tail. It is a surfactant.
  • DTAB- Has a hydrophobic tail and a hydrophilic
    head
  • Nisin- a natural antimicrobial agent used as a
    lantibacterial
  • Lysozyme- causes hydrolysis of bacterial cell
    walls, fights against bacterial infections
  • Albumin- Protein manufactured by liver, helps
    fluid remain in the blood stream
  • Phosphate Buffer- a salt or ester of phosphoric
    acid (a colorless liquid used in pharmaceuticals)
    that minimizes change in the acidity of a
    solution when an acid or base is added

6
Analysis
  • DTAB and Nisin worked best when killing
    pediococcus, a gram positive bacteria
  • Only high concentration DTAB, and low
    concentration lysozyme, killed E. Coli
  • For Nisin
  • Low concentration was more effective
  • The wet tubing killed more bacteria than the dry
  • The SDS high and DTAB low concentrations showed a
    larger kill zone for the dry tubing than the wet
  • Albumen did not bond with gram positive bacteria
  • Works well when it does
  • Our second trial had better results
  • Tubing was fully sterilized
  • Phosphate buffer was used as a control so it did
    not kill bacteria.

7
Thank You
  • To our mentors, Alia Mulder, Danielle Leiske, and
    David Pulitzer for teaching us the concepts used
    in our experiments.
  • To Dr. Skip Rochefort for leading us in this
    great learning adventure that will change us
    forever, we will never forget you!
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