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Counting of Helminthes Eggs

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Counting helminthes eggs in feces Eggs counts can be value in epidemiological surveys. The intensity of an intestinal helminthes infection may sometimes be indicated ... – PowerPoint PPT presentation

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Title: Counting of Helminthes Eggs


1
  • Counting of Helminthes Eggs

Stolls method
2
Counting helminthes eggs in feces
  • Eggs counts can be value in epidemiological
    surveys.
  • The intensity of an intestinal helminthes
    infection may sometimes be indicated by the
    concentration of its eggs in feces.
  • Egg counts provide a reasonable estimate of the
    numbers of adult worms present.
  • Egg count before treatment may help determine
    whether treatment is needed and counts after
    treatment assess its success.
  • The approximate number of eggs per gram of feces
    can be calculated by using formal ether
    technique.
  • When a more accurate count is required the
    Stolls method can be used.

3
Stools technique procedures
  • Weight 3 grams of feces in a screw cap container.
  • Add 42 ml of water to give (1/15) dilution of the
    feces.
  • If the feces are formed specimen, use sodium
    hydroxide 0.1mol/l solution instead of water.
  • Using a rod, break up the feces and mix it with
    the water.
  • Cap the container and shake hard to complete in
    mixing.
  • Using a graduated plastic bulled pipette, or a
    Pasteur pipette marked to measure the required
    volume, pick 150 µl of the suspension and
    transfer this slide.
  • Cover the slide with long coverslip if available,
    or two squares coverslips.
  • Examine systematically the entire preparation,
    using 10x objective. Include the count any eggs
    laying outside the edges of the coverslip because
    these are contained in 150 µl sample.

4
  • Multiply the number of eggs counted by 100 to
    give the number of eggs per gram of feces.
  • If the specimen isnt formed, the following
    additional calculation is necessary to give the
    number of eggs per gram.
  • Fluid specimen --------------------------------
    5
  • Unformed watery specimen ------------------- 4
  • Unformed soft specimen ----------------------- 3
  • Semiformed specimen -------------------------- 2

9. Calculate the number of eggs per day, by
multiplying the number of eggs per gram by the
total weight of 24hrs fecal specimen.
5
  • Calculate the number of burden worm by dividing
    the number of eggs per gram on number of eggs
    the parasite laying per day.

NOTE Female Ascaris pass about 200,000 eggs per
day, Nectar pass about 9000 eggs per day, female
H.nana 7000 and female Trichuris pass about 5ooo
eggs per day .
6
Interpretation of result
  • The Center For Disease Control, Atlanta

Protozoa Helminthes
Rare 2-5 / coverslip 2-5 / coverslip
Few 1 / 5-10 HPF 1-2 / LPF
Moderate 1-2/ HPF or 1/ 2-3 HPF 1 / 5-10 HPF
Many Several / HPF Several / LPF
7
  • Intestinal Nematodes

Ascaris lumbricoides giant round worm
8
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9
Ascaris lumbricoides
Ascaris lumbricoides is the giant roundworm of
human, inhabit small intestine and cause
Ascariasis .
  • Infective stage Embryonated eggs.
  • There are three diagnostic stages for
    Ascaris lumbricoides in stool adult, Fertilized
    eggs, Unfertilized eggs.
  • DUE TO LARVAL MIGRIATION STAGE, Sputum sample
    may be collected on persons suffering from
    infection with Ascaris lumbricoid.

10
Ascaris lumbricoides
Ascaris adult worms are the largest known
intestinal nematodes
  • Adult (large size )
  • Male has a coiled posterior end, 2 minute
    spicules and no copulatory bursa 15-30 cm in
    length.
  • Female oviparous have straight, pointed tail and
    no spicules. (lay 200,000 eggs daily) 20-40 cm
    in length.

11
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12
Ascaris lumbricoides Adult
13
  • There are 3 shapes of ova
  • Embryonated egg
  • Fertilized egg
  • Unfertilized egg

embryonated egg
14
Ascaris lumbricoides Unfertilized Egg
Ascaris lumbricoides Unfertilized Corticated Egg
Ascaris lumbricoides Unfertilized deCorticated Egg
15
  • Plant cell resemble helminth eggs particullay
    Ascaris lumbericoid unfertilized egg. This
    artifact is typically round to oval in shape and
    may or may not have a definite cell wall,The
    interior of the cell looks like a cluster of odd
    shaped vacuoles.
  • Unfertilized eggs a thin shell protects the
    inner a morphous mass of protoplasm,The shell
    layers of the egg provide a very resistant
    structure which can withstand many chemicals
    which make them ideal parasites of the intestine.

16
Ascaris lumbricoides Fertilized Egg
Ascaris lumbricoides Fertilized corticated egg.
Ascaris lumbricoides Fertilized Decorticated Egg
17
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18
Ascaris lumbricoides Embryonated Egg
Ascaris lumbricoides Embryonated Decorticated Egg
Ascaris lumbricoides Embryonated Corticated Egg
19
Pollen grain in a concentrated wet mount of
stool. This grain looks very similar to the
fertile egg of Ascaris lumbricoides, although the
spine-like structures on the outer layer should
separate the two.
fertile egg of Ascaris lumbricoides
20
Plant element
21
Ascaris Lumbricoides Larvae
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