Top Ten List

1
Chapter 21
Viruses Associated with Enteric Infections
??????

• Human enterovirus

• Viruses of causing acute gastroenteritis

2
Hnman enteroviruses
Poliovirus
(types 13)
Coxsackievirus
group A types 122, 24 group B types
16
Enteric cytopathogenic human orphan(ECHO)
virus
(31 types)

New enteroviruses
(types 6871)
3
1.Biological properties

of enterovirus
? Shape and structure
d2430 nm, spherical and naked virus with
icosahedral nucleocapsid
genomessRNA


polyprotein ?

VP1VP4 and functional protein
????(VP1?????)
4
? Type-specific antigen
D(density)/N(native) antigen
C (coreless) antigen
? Rresistance
(stronger)
(pH39??,???????????)
? Cultivation
primary cell culture----CPE animal
inoculation (A1,A19,A22)
5
2.Pathogenicity of enteroviruses
? Replicate in the intestinal tract and spread
through bloodstream to target organs
(?????????)
? Via fecal-oral route cause a variety of
diseases or syndromes

---from subclinical infection to severe
infection of CNS
(??????????)
????????????????,?????????????????
P275276?
6
? Poliovirus (neurotropic virus)
---pathogen of poliomyelitis(???????????)

• source of infection (patients and carrier)
• cause poliomyelitis by fecal-oral route
• clinical findings of poliomyelitis

subclinical infection(90)
abortive infection (5)
nonparalytic poliomyelitis/aseptic
meningitis(1-2)
paralytic
poliomyelitis(0.1-2)


nonparalytic paralytic1001000 1
7
?Coxsackievirus and ECHO virus
---cause a variety of diseases or syndromes (such
as aseptic meningitis and
encephalitis and so.on) (P275276?)
? Enterovirus 71
----The main cause of acute hemorrhagic
conjunctivitis (AHC)

initial site of infection and route of
transmission E70,CA24 and Ad3,7?AHC (????????)
8
? Enterovirus 71
(neurotropic virus)
---cause viral CNS disease (such as aseptic
meningitis and encephalitis and so.on) and
HFMD(hand-foot-and-mouth disease,????)
3.Immunity of enteroviruses
--- lifelong type-specific immunity (
neutralizing antibody )
9
4.Laboratory diagnosis
of enteroviruses

• Isolation and identification of virus
• Detection of viral antibody and RNA
  

(CPE)
5. Specific prevention of poliovirus
? Artificial active immunity ? Artificial
passive immunity
?????????OPV?????????????(VAPP)????
IPV ( Salk vaccine) OPV(Sabin
vaccine )
10
Gastroenteritis Virus

• ---Viruses of causing
• acute gastroenteritis

• Rotavirus (RV)
• Other acute gastroenteritis virus

11
Rotavirus
(????)
1.Shape of virion
---d60-80nm, spheric and naked virus with
double-shelled capsid
2.Genome

• ? consists of 11 segments of ds-RNA
• ? code for 6 structural proteins(VP1-4,6,7) and 5
  non-structural proteins (NSP1NSP5)

appearance of a wheel
12
3.Pathogenicity
? Source of infection ? Route of transmission ?
The main viral cause
of
acute gastroenteritis
group A main pathogen of acute
gastroenteritis in infants group B cause
epidemic diarrhea in adult group C cause
human or animal sporadic diarrhea
(6??2?)
(or ADRV)
13
4. Diagnosis of infection
? Detection of viral particle and viral
antigen (EM,ELISA) ? PAGE RT-PCR

(?????????)
14
2.Other acute gastroenteritis virus
?Cause viral gastroenteritis by fecal- oral
route
EAd-40, 41 and 42??????????????????

• ?Including
• Enteric adenovirus
• Calicivirus

???????Norwalk ?? ????????????????? ?????
?? ?????????


SRSV (small round structured virus) classic
calicivirus (Classified as HuCV)
?5???????
Astrovirus
?????5???????(520?????) ?????