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Research for Agricultural Development Program (RADP)

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Title: Research for Agricultural Development Program (RADP)


1
(No Transcript)
2
Research for Agricultural Development Program
(RADP)
  • Pakistan Agricultural Research Council
  • Islamabad

3
PROGRAM PROFILE
  • Approved by ECNEC 7th March 2007
  • Start 3rd April 2007
  • Duration 5 years
  • Extension (1st ) For 2 years (upto 30th June,
    2013)
  • (2nd ) For 2 years again (upto 30th June,
    2015)
  • TOTAL Project Cost 2,963.00 (Rs. million)
  • Local cost 2,087.00 (Rs. million)
  • Foreign Exchange 876.00 (Rs. million own
    resources)

4
Program Objectives
  • To address the current and emerging needs of
    science based-agriculture development, food
    security, poverty reduction, economic efficiency
    export competitiveness.
  • To serve as a mechanism for timely response to
    emerging research issues (Pest/disease epidemics,
    nutrient deficiency, climate change)
  • Develop technologies for maximizing productivity
    (per unit Land, labour, water, capital etc)
  • Move from research output to innovations as
    products and services for small holders

5
Scope of RADP
  • Research Priority research themes (22) Plant
    Sciences 7, Natural Resources 4, Animal
    Sciences 4 and Social Sciences 7
  • Infrastructural development Up gradation of
    research labs, green houses and other facilities
    at research establishments
  • Collaboration International and national
    research collaboration.
  • Training Human resource development

6
Project Directors of RADP
Name Tenure
Dr. Nisar Ahmad Jan, 2008 to Aug 2012
Dr. Sher Muhammad Sept., 2012 to Aug 2013
Dr. Sarfraz Ahmad Aug, 2013 to date
7
Management/Implementation
A. Program Steering Committee (PSC)
  • Terms of Reference
  • Overall guidance, supervision and
    monitoring of program
  • Resolve Operational and Financial issues
  • Authorize technical revision
  • Re-appropriation of funds within the
    approved cost and scope
  • Composition
  • Secretary, Ministry of NFSR Chair
  • Chairman, PARC Member
  • Additional Secretary-I, Ministry of NFSR Member
  • Additional Secretary (Exp) Finance
    Division Member
  • ADC, Ministry of NFSR Member
  • Technical Members of PARC Member
  • Member (Finance), PARC Member
  • Chief (Agriculture) PD Division Member
  • Vice President (PCCC) Karachi Member
  • DG (PD), PARC Member
  • Project Director (ME) Ministry of NFSR Member
  • Project Director, RADP Secretary

8
  • B. Program Executive Committee (PEC)
  • Terms of Reference
  • Review and approve research
    programs/projects
  • Review annual and mid term progress
  • Identify and accommodate new emerging
    research areas
  • Identify and approve training needs etc.

Composition
  • Chairman, PARC Chair
  • Technical Members of PARC Member
  • ADC, Ministry of NFSR Member
  • JS (Plan) Ministry of NFSR Member
  • DG (PD), PARC Member
  • Project Director Member / Secretary

9

Project Progress A). Financial Phasing, Release
and Expenditure
(Rs. Million)
Item PC-I (Cost) Allocation Release Expenditure
2006-07 (one month) 268.909 200.00 40.00 39.961
2007-08 1030.423 948.423 535.635 353.442
2008-09 809.181 600.000 239.058 239.058
2009-10 504.568 400.000 160.000 160.000
2010-11 349.919 411.555 120.501 111.725
2011-12 - 165.321 107.458 92.868
2012-13 - 170.00 74.00 73.156
2013-14 - 230.00 46 40.00
2963 3125.299 1322.652 1110.21
() 2nd quarter release of Rs. 46 million under
process
10
Total Project Funded under RADP
  • Completed Research projects 82
  • Ongoing research projects 27
  • Total 109

11
Pre-DevolutionB (i) Sub-Research
Projects/Activities Funded under RADP upto 30th
June, 2011
(Discipline Wise)
Sr. No. Discipline Completed
1 Crop Sciences 20
2 Natural Resources 6
3 Animal Sciences 5
4 Social Sciences 9
Total Total 40
12
Post-DevolutionB (ii) Sub-Research
Projects/Activities Funded under RADP from 30th
June, 2011 to 30th Nov., 2013
(Discipline Wise)
Sr. No. Discipline Completed On-going Total
1 Crop Sciences 20 18 38
2 Natural Resources 10 5 15
3 Animal Sciences 8 - 8
4 Social Sciences 4 4 8
Total Total 42 27 69
13
Brief Research Outputs
14
Crop Sciences
  • Germplasm of crops acquired, characterized for
    breeding/hybrids development (gt15,000).
  • Sunflower hybrids (SMH 0907, SMH 0917) with yield
    potential 3628-4730 kg/ha, early maturity, short
    statured.
  • Canola hybrid (CRH-40/10) with yield potential
    3167 kg/ha.
  • Short duration ground nut variety (PG-1058)
    110-120 days maturity developed (potential 2.6
    t/ha).
  • Four tomato hybrids with potential 59-69 t/ha,
    under test for yield stability.

Cont
15
Crop Sciences
From Pre-Page.
  • Mandarin hybrids (early maturity, good quality
    and less seed in final evaluation).
  • Fast track production of hybrids of cotton (05
    hybrids under evaluation), wheat, rice and maize
    launched.
  • Sorghum-Sudan grass hybrids (02) yield
    potential140-145 t/ha.
  • Salt, drought, heat and rust resistance /
    tolerant lines of wheat identified and available
    as valuable material for further breeding.
    Transgenic rice and wheat resistant to salts
    under test.
  • Sugarcane varieties characterized for flowering
    ability, areas identified for viable fuzz
    production and varietal development.

Cont
16
Crop Sciences
From Pre-Page.
  • 50000 micro-tubers potato produced, of this 35000
    stored and 15000 used for seed multiplication of
    disease free seed at Kaghan.
  • 14 ginger and turmeric lines from Sri Lanka,
    China acquired. These lines are under cultivation
    in green house and open field for multiplication.
  • Technology for domestication of wild growing
    black cumin developed.
  • Dates processing (solar drying), and packing
    technology introduced.
  • Mass rearing of bio-control agents - Insectary
    established.
  • Progeny garden of 12 tea cultivars established at
    Shinkiari, Mansehra. Potential cultivars are
    under multiplication.

Cont
17
Crop Sciences
From Pre-Page.
  • 600 demonstration plots of vegetable gardening in
    conflict areas of Swat developed for livelihood
    improvement.
  • Mango harvesting and pre cooling machine, buffalo
    milking machine and flatbed maize/ground nut
    dryers developed in collaboration with local
    manufacturers.
  • Spawn production for mushroom cultivation -
    Products marketed.
  • Development of small olive oil extraction plant
    for use at local level
  • Transfer of rodent control technologies through
    commercialization and services in Sindh.
  • Three pilot plants one each for processing of
    organic compost, bio-herbicides and
    bio-pesticides locally fabricated, their products
    to be used for organic farming.
  • Policy analysis reports on pest control, pest
    risk analysis and pesticides residues for trade
    policy and management interventions.

18
Milking machine
Olive extraction plant
19
Bio pesticides plant
20
Natural Resources
  • Technologies of chemical and bio logical
    reclamation of salt affected land in Khyber
    Pukhtunkhwa demonstrated on farmers field.
  • Developed bio fertilizer processing at NARC.
    Plant has capacity of to manufactured 200 bags of
    organic fertilizer / day.
  • Bed planting with hybrid rice using mechanized
    process, saved 35 water as compared to
    conventional methods.
  • Bio-remediation technology established at NARC.

Cont
21
Natural Resources
From Pre-Page.
  • Honey analysis lab established at NARC.
    Technologies refined for commercialization of
    valuable bi-products of honey (royal jelly,
    pollen and bees wax etc).
  • Production and up-scaling of bio-fuel plants
    Jatropha, Sukhchen and Castor Beans (Bahawalpur,
    Umerkot, D.I. Khan, SARC).
  • Quality deciduous fruits propagation in Gilgit
    and Rawal sheds.

22
Animal Sciences
  • With stair-step feeding regime conception in
    Nili-Ravi buffalo and Sahiwal heifers achieved in
    18-24 months without any performance losses.
  • Heat synchronization and improved semen
    preservation of buffalo and goat lead to improved
    conception through AI.
  • Viruses strains identified for vaccine
    development to control avian influenza (poultry)
    and FMD (ruminants).

Cont
23
Animal Sciences
From Pre-Page.
  • Research on control of disease (Lernea, Argulus,
    Dropsy, Gillrot, Finrot) of fish in pond culture
    and trout culture.
  • Research on captive breeding of endangered wild
    animal for conservation and cross breeding.
  • Enhanced milk and meat production of goats
    through feeding regime.
  • AI in goats successful first time.

24
Social Sciences
  • Survey and analyses of the commodity value chain
    (poultry, dairy, fruits), agricultural growth and
    poverty reduction, food consumption diversity,
    harvest and post harvest losses.
  • Generated useful information and data sets on
    these issues for planning and policy making.
  • Capacity building of scientists, researchers,
    educators, policy makers and community workers
    Five courses 200 participants.

Cont
25
Social Sciences
From Pre-Page.
  • PARC Institute of Advanced Studies in Agriculture
    - (NUAS) established at NARC. Enrolled 306 Ph. D
    (87), M. Phil (219) students.

26
Research to Commercialization
  • 30 ton hybrid of Canola produced and sold through
    PATCO.
  • 1.2 ton of Sunflower hybrid produced and sold
    through PATCO.
  • Black Cumin production technologies.
  • Vegetable seed production for kitchen gardening.
  • Bio-pesticides products.
  • Bio fertilizers formulation.
  • Honey and byproducts.
  • Bio-remediation technologies.
  • Small scale Olive oil extraction machine.
  • Picking and pre cooling technology of Mango.
  • Milking machine for water buffalo.

27
Research to Commercialization
  • Feed formulation for trout fish.
  • Ornamental fish production technologies.
  • Grain baits for rats and mice. Pellets for rats.
  • Food and Mouth Diseases (FMD) and Avian Influenza
    vaccines.
  • Dates Solar dryer .
  • Mushroom production technologies.
  • Mobile Groundnut and Maize dryer.

28
B (iii) Lab Equipment/Farm Machinery/Vehicles
Sr. No. Narration Provision Provision Procured Procured
Sr. No. Narration Total Items Cost (Rs. Million) Total Items Cost (Rs. Million)
1 Lab /Field Equipment 2694 528.60 789 257.045
2 Office Equipment 277 18.240 200 14.942
3 Replacement of lifts 02 15.00 02 6.930
4 Telephone Exch. 02 30.00 02 36.363
5 Farm Machinery /vehicle 157 169.13 110 126.502
Total 3132 760.97 1103 441.782
Vehicle details given in next slide
29
B (iv) Infrastructure Development a. (Repair
Maintenance)
Work Location PC-1 Cost (Rs. Million) Completion Cost (Rs. Million) Status
Hostels NARC, Islamabad 3.15 2.70 Completed
CSI Building -do- 3.0 (R) 3.13 -do-
BARD Building -do- 4.69 2.53 -do-
Staff Colony -do- 2.00 1.60 -do-
Officer Colony -do- 3.00 2.57 -do-
Farm Roads -do- 42.95 39.81 Under arbitration
Rehabilitation of NSCRI NSCRI, Thatta, Sindh 10.19 10.50 completed
Repair Maintenance of National Herbarium NARC Islamabad 3.00 1.57 completed
HVAC system NARC auditorium 3.20 3.20 completed
Total (A) 75.18 67.61
() PSC authorized in 3rd meeting () PSC
authorized in 5th meeting
30
b. New Construction
Rs. Million
Work Location PC-1 Cost Completion Cost Status
Buffalo Sheds NARC, Islamabad 4.19 5.76 Completed
10 F-type residences -do- 7.28 6.08 -do-
Parking Sheds -do- 2.43 2.10 -do-
Boundary wall -do- 25.45 26.052 -do-
Pumping Machinery Installation 5 T. Wells -do- 13.00 11.53 -do-
Social Sciences Institute -do- 22.01 36.25 -do-
Girls Hostel -do- 17.46 21.89 -do-
Const. of Lab. MARC, Gilgit MARC Gilgit. 2.15 2.70 -do-
Glass House / Insectary/ Wildlife Enclosures NARC, Islamabad 31.730 25.90 -do-
Community Center / Café NARC Islamabad. 25.19 47.670 Work in progress
Construction of lab. Building LRS, NARC 7.79 16.724 Work in progress
Total (B) 158.68 202.656
G. Total (A B) 233.86 270.266
31
Social Sciences Institute, NARC
32
Girls Hostel
33
10 F Type residences at NARC
34
5 Tube Well at NARC
35
Work Plan (2013-14)
  • i) Sub-Research Projects/Activities
  • On-going 27
  • New Launched 04
  • Adaption and indigenization of household levels
    solar desalination and water from air
    technologies. Rs. 3.5 million.
  • Development of clean and true to type
    horticultural crops nursery in Islamabad Capital
    Territory (ICT). Rs. 9.89 million
  • Establishment of mother blocks, fruit plant
    nurseries of high value crops and seedlings/seed
    production of economically important vegetables
    in District Mansehra. Rs. 6.0 million
  • Establishment of model facility of wastewater
    treatment through bioremediation at rose and
    jasmine garden facility Islamabad. Rs. 3.495
    million

36
  • Civil Work in 2013-14
  • Construction of Community Center Cafeteria at
    NARC Rs. 53.00 M.
  • Construction of LRS Lab at NARC Rs. 16.724 M.
  • Construction of boundary wall around CARS,
    Karachi Rs. 9.40 M

37
Way forward
  • To address the emerging challenges of food
    security and self reliance in agriculture
    continuous financial support from PSDP is
    mandatory.

38
Thanks
39
Completed Research Sub-Projects of RADP
40
CROP SCIENCES
41
  • Development Improvement of Mass Production
    Techniques of Insect Bio-control Agents Dr.
    Ehsan-Ul-Haq, PSO, IPMP,IPEP, NARC
  • Achievements
  • Insectary Biological control lab for rearing the
    bio-control agent and their insect host.
  • Host of Trichograma (Sitotraga) raised in chamber
    (parasitoid) where adults collected automatically
    from the chamber (Root borer, stem borer, top
    borer and Gurdaspur borer).
  • Reared Chrysoperla (Predator white fly, aphids,
    thrips, mites etc) and Coccinellid (white fly,
    mites) and aphidius (aphid C) on different hosts,
    aphids, sitotraga and mealy bugs.
  • Selected different host rearing for mass
    production of these bio-control agents in the
    laboratory. Sitotraga and mealy bug found most
    efficient for Chrysoperla and coccinellid.
  • Koopert ( Neitherlans), Syngenta (U.K), Biobest
    (Switerland) mass production (canada, America)

42
  • Development of sunflower and canola hybrid and
    canola type mustard varieties (Second Phase), Dr.
    Muhammad Ayub Khan, CSI, NARC
  • Achievements
  • Sunflower
  • Two potential sunflower hybrids, SMH-0907
    (PARSUN-3) and SMH-0917 were tested under
    different environmental conditions of the
    country. All hybrids were tested during four
    growing seasons (i.e. 2 spring and 2 autumn). On
    the average of three seasons SMH-0907 and
    SMH-0917 produced 3534 and 3115 kg/ha,
    respectively. Whereas, commercial imported
    hybrids, Hysun-33 and NK-S-278 produced 2856 and
    2998 kg/ha, respectively.
  • A total of 1.8 ton hybrid seed of two potential
    hybrids (SMH-0907 and SMH-0917) was produced
    during 2012 and distributed among farmers on 50
    less price than imported hybrid seed.
  • SMH-0907 has been recommended by the Variety
    Evaluation Committee for registration. However,
    advised to submit data of 2nd seasons on
    morphological description of hybrid and parent
    lines. Data have been recorded during spring,
    2013 and is in process of compilation. The hybrid
    has been renamed as PARSUN-3.
  • Canola
  • Performance of PARC Canola hybrid was almost
    equal (1992 kg/ha) as compared to check,
    Hyola-401 with 1995 kg/ha, However, SPS N7/28
    produced higher yield (2067 kg/ha).

43
  • Cont
  • Seed of parent lines of potential hybrid was
    multiplied at NARC and Kaghan. A total of 750 kg
    seed of PARC canola hybrid was also produced at
    NARC.
  • Screening of potential sunflower and canola
    hybrids against major insect pests and diseases .
  • Proposals for approval of PARC canola hybrid was
    presented before Variety Evaluation Committee
    (VEC), however, committee advised to present
    again due to some deficiencies. Therefore, the
    proposal will be presented again in August, 2013.
  • 30 tons of PARC Canola Hybrid seed has been
    marketed during 2011-12 and 2012-13 rabi seasons.
  • 1.2 ton of Sunflower Hybrid (PARSUN-3) has been
    marketed during spring, 2013.
  • Private sector has been involved in promotion of
    sunflower and canola hybrids through planting of
    demo plots of local hybrids on farmers, field.

44
  • Evaluation of Locally Developed Mandarin Hybrids
    in Potential Citrus Growing Areas (Phase-II) Mr.
    Mukhtar Ahmed, SSO, Fruit Crop Research
    Programme, HRI, NARC
  • Achievements
  • Two hybrids NARC 05-18 and NARC 05-17 seem a
    promising in respect of fruit production and
    quality parameter (weight, juice content, TSS and
    acidity).
  • NARC 05-18 and NARC 05-17 had the maximum average
    fruit weight of 178 gm and 170 gm as compare to
    Kinnow 164 gm.
  • Higher TSS was recorded in NARC 05-17 (11.86)
    followed by NARC 05-18 (11.80) compared Kinnow
    with TSS value of (11.30).
  • NARC 05-18 had acid content of 0.96 which was
    lower than Kinnow (1.04) fruit harvested on
    10-12-2012.
  •  Propagation
  •  300 plants of NARC 05-18 and NARC 05-17 were
    grafted
  •  Seedless/Less Seeded Mandarin Hybrids
  • 63 seedless/less seeded hybrids were developed
    through crosses between Kinnow xTarocco (orange),
    NARC 05-18 x Tarocco and NARC05-17x Sanguinello
    (orange) and planted in fruit area HRI, NARC for
    evaluation studies. The plants will start bearing
    fruit after 4 to 5 years.

45
  • Adoption and Commercialization of a Small-Scale
    Olive Oil Extraction Unit, Mr. Liaqat Ali Shahid,
    Principal Engineer, ABEI, NARC
  • One small scale olive oil extraction plant
    imported, tested/evaluated and demonstrated at
    NARC.
  • Demonstrations at farmers fields conducted with
    oil recovery of up to 19.38 observed, oil
    analysis done, overall processing capacity has
    been enhanced by modifying centrifuge. Operation
    cost found at Rs. 9.25/kg.
  • Improvements designed and MoU for local
    manufacturing with Pvt. sector signed with a firm
    in Faisalabad for commercialization. (M/s
    International Technology)
  • Demonstration of prototype has been carried out.

46
  • Improvement of Groundnut for short duration and
    yield in rainfed wheat cropping system Malik Shah
    Nawaz, PSO, Oilseed, CSI, NARC
  • Achievements
  • 134 short duration exotic / local lines were
    evaluated.
  • 1 short duration line PG-1058 of duration 110-120
    days has been developed.
  • 2 short duration lines PG-963 and PG-1133 of
    100-110 days have also been identified.
  • 22 desirable parental lines crossed and F2, F3
    and F4 generations are in field
  • Further short duration lines will be selected
  • Agronomic and on farm yield trials were
    conducted at NARC-Islamabad, Chakwal, and Attock.

47
  • Intra and inter-specific variation of oilseed
    brassicas using biochemical and molecular
    markers. Dr. Ashiq Rabbani, PSO, IABGR, NARC
  • Achievements
  • Acquired 2040 germplasm from abroad and 525 from
    Pakistan.
  • 22 elite lines of oilseed group and 13 vegetable
    type identified and seed multiplied.
  • 15 lines identified for high yield, oil content,
    low level of erucic acid, glucosinolates.
  • Screening of drought tolerance of 300 accessions.
  • 2 aphid resistant lines of brassica and 3
    non-shattering lines of canola also identified.

48
  • Marker assisted selection genomic studies on
    stress tolerance in wheat and rice.
  • Dr. Ghulam Muhmmad Ali
  • Achievements
  • Salt tolerant wheat and rice genotypes were
    identified at germination, vegetative growth
    stage and at maturity. These genotypes could be
    used for salt tolerance improvement in
    hybridization with elite cultivars using
    molecular markers aided selection using SSR
    markers identified in the project.
  • Lasani and Inqilab wheat varieties have been
    transformed with salt and drought tolerance gene.
    The transformed lines are at T2 stage. Bioassay
    of these lines have shown that these lines are
    relatively better tolerant to drought stress
    maintaining higher turgor and photosynthetic rate
    under drought.
  • JP-5 and KSK-282 rice varieties have been
    transformed with salt tolerant gene. The
    transformed lines are at T4 stage. Bioassay of
    these lines have proven their better salt
    tolerance ability.

49
  • Development of Sorghum Sudangrass hybrids for
    high forage yield and quality characters (2nd
    Phase) Dr. M. Shafiq Zahid, PSO, Fodder Program,
    NARC
  • Achievements
  • Two promising hybrids lines (A, B R) were
    planted at different locations for
    purification/maintenance purposes. About 464 kg
    pure seed of parental lines (A, B R) of two
    hybrids has been produced that is sufficient to
    develop hybrid seed over an area of 100-150.
  • Two hybrids (NARC hybrid-2 and NARC hybrid-4)
    were planted at 7 different locations. About 67
    kg seed was produced and has been utilized for
    adaptability, agronomic trials, demonstrations
    and registration purposes. The other most
    suitable sites are Faisalabad, Sargodha,
    Arifwala.
  • The data of 4 cuttings indicated that NARC
    Hybrid-2 and NARC Hybrid-4 were among the
    top-yielding hybrids by producing 138 129 t
    ha-1 green fodder yield, whereas it was 111t
    ha-1 in check (Pak-Sudan).
  • Correspondence work for registration is in
    progress with FSC RD. Seed of two registered
    hybrids and their parents were provided to FSCRD
    for 2 years for characterization and DUS data
    generation. Required data for characterization
    generated at NARC has been provided to FSCRD.

50
  • Characterization of sugarcane germplasm for
    flowering ability (NARC Unit) Dr. Muhammad
    Zubair, PSO, CSI, NARC
  • Achievements
  • 450 sugarcane germplasm lines were evaluated for
    flowering ability at three locations (Sugarcane
    breeding station-Murree, Sugarcane breeding
    station-Dargai, NSCRI-Thatta.
  • At SBS Murree and SBS, Dargai, 10-20 varieties
    flowered. Only thin varieties flowered with
    flowering intensity of 5-30. The fuzz (Sugarcase
    Seed) produced had very poor viability
    (0.1-1.0). These two stations are being run by
    provincial governments proved non-viable for
    sugarcane breeding under natural conditions.
  • At NSCRI-Thatta, 40 variety flowered with 40-80
    flowering intensity. Fuzz (Sugarcane seed)
    produced had 7-10 viability. Sugarcane breeding
    is possible at this location by providing
    photoperiod and temperature control chambers.

51
  • Identification/Selection of parental lines and
    hybrid development in tomato (PGRP) Dr. Abdul
    Ghafoor, PSO, IABGR, NARC
  • Achievements
  • Genetic resources increased from 100 to 875 in
    the gene bank.
  • Among the germplasm evaluated, 30 genotypes were
    identifed as high yielding and out of these 14
    were used in hybridization, and 130 combinations
    were obtained.
  • Four hybrids were observed promising and the
    genotypes used in these hybrids were provided to
    the vegetable program.
  • Thirty genotypes were screened against salinity
    under hydroponic and three genotypes (019291,
    017909, 017869) were observed tolerant at 100mM
    Na2Cl.
  • Bio-chemical and Molecular analysis conducted.
  • More than 600 genotypes of tomato germplasm were
    distributed to ten researchers for their research
    including vegetable program and NIAB, Faisalabad.
    The germplasm (gt 300 accessions) were provided to
    students for their degree research program.
  • One PhD, one M Sc, two internees were involved in
    the project period.
  •  

52
  • Identification/Selection of parental lines and
    hybrid development in tomato (Vegetable) Mr.
    Muhammad Farooq Chaudhry, PSO, HRI, NARC
  • Achievements
  • Developed four local hybrids with yield range
    (54.5-60.52 t/ha).
  • Developed technology for propagation of tomato
    hybrid plants through cuttings which gave good
    yield compared to plants through seedlings. Also
    a NPK dose (1507575) was standardized for their
    best performance.
  • Selected eleven parental lines for tomato hybrid
    development.
  • For commercialization of developed hybrids, MOU
    was signed between PATCO and 4-Brothers Private
    Company.

53
  • Accelerated Development of Hybrid Wheat, Rice,
    Cotton, Sunflower, Non Shattering Canola, Maize
    and Use of Innovative Technologies, Dr. Ahmed
    Bakhsh Mahar, PSO, CSI, NARC
  • Achievements
  • Cotton
  • 65 hybrid combinations developed and 5 high
    yielding selected. 2000 kg hybrid seed produced.
  • Among five Gametocides, 2-4-D induced male
    sterility with toxic effect at all 20
    concentrations (0.2 to 5.00 ppm).
  • CMS lines (G-97 G-72) identified from Chinese
    germplasm.
  • Seven Genetic Male Sterility (GMS) lines acquired
    from CCRI Multan and multiplied.
  • Wheat
  • Out of 96 cross combination three found
    promising.
  • BAU 9403 was identified as chemical hybridizing
    agent with a dose of 300 gm BAU per acre.
  • Increase in dose of BAU 9403 reduced seed setting
    on sterile plants. BAU caused delayed maturity.
  • Seed setting with BAU 9403 ranged from 33 to 61.
  • 0.5 tons hybrid seed was harvested with the
    application of BAU 9403.

54
  • Cont.
  • Embryo Rescue
  • Tissue culture protocols were optimized for
    growing immature embryos.
  • 15 days old embryos of wheat variety NARC 2009
    exhibited good regeneration response on MS medium
    under dark condition.
  • Embryos of maize gave good regeneration response
    on B-5 medium.
  • 30 days old hybrid canola embryos showed better
    regeneration activity.
  • Maize embryos started germination 4 days after
    culture.
  • In vitro root formation was achieved in
    sunflower, maize, brassica and tomato.
  • Cuttings of cotton mother plants failed to
    produce roots on various media.
  • Precocious germination of 20 days old cotton seed
    was recorded.

55
  • Sustainability Improvement of Tea Production
    Mr. Abdul Waheed, SSO, NTRI (PARC), Shinkiari,
    Mansehra
  • Achievements
  • Indigenous germplasm of 3 clones (P-8, P-9, and
    P-3 of North Carolina) from uni-lever acquired
    for evaluation. Quick selection and screening
    protocol of the clones.
  • Experiments on 64 elite tea bushes initiated.
  • Progeny garden of 12 cultivars established. Out
    of 12, 3 are disease resistance and other 9 are
    relatively susceptible.
  • 3-4 acres of tea garden is established at NTRI,
    Mansehra.
  • The developed material tested on high elevation
    i.e. AJK.
  • Experiments on inter cropping initiated.

56
  • Ginger and Turmeric Introduction, Acquisition,
    Kitchen gardening and Farm Production Technology
    Dr. Ghulam Mustafa Sajid, PSO, PGRP, IABGR, NARC
  • Achievements
  • 14 ginger and turmeric lines from Sri Lanka,
    China acquired.
  • These lines were cultivated in green house and
    open field. More than 300 ginger plants prepared
    in clay and plastic pots. Growth is good in clay
    pots for kitchen gardening. 2 training imparted
    for private farms and 2 for general public.
    Package of technology to develop with plants for
    farmers and kitchen gardens.
  • In-vitro culture established in lab, micro
    propagation started.

57
  • Fabrication of Liquid Bio-Herbicide Processing
    Unit Dr. Yousaf Hayat, PSO, EBI, NARC, Islamabad
  • Achievements
  • Bio-herbicide processing unit has been fabricated
    at NARC. Bio-herbicide is formulated from natural
    plants and weeds.
  • The product tested against different weeds and it
    has ability to control pre - emergence weeds.
    This is the new intervention for organic growers
    in the country. Some plants have been identified
    like Casia fistula , Lintana, Chinopodium,
    Calotropis, Euphorbia etc.

58
  • Fabrication of liquid bio-pesticide and micro
    nutrient formulation unit Mr. Azhar Javed, SSO,
    NARC
  • Achievements
  • Bio-pesticide and micro nutrient plants
    fabricated at BARD yard NARC for formulation of
    bio-pesticide and organic salt.
  • Different organic pesticide formulations have
    been tested against different insect pests
  • Products have been standardized and are being
    sold for insect pest control. The most effective
    bio-pesticides are derivatives / decoction of
    garlic, turmeric, hot chillies, ak and some
    others weeds .

59
  • Fate of pesticide residues in cotton
    agro-ecosystem and their impact on human health
    and livestock Dr. Karam Ahad, SSO IPEP, NARC
  • Achievements
  • 249 samples of different matrices like water,
    soil, vegetables, feed/fodder and milk analysed
    showed varying level of pesticide residues
  • During pre-cotton season 20 samples above
    MRL(0.5 microgram/l-EU) but during post cotton
    MRL in 95 water samples
  • In 91 soil samples the most abundant compound
    was fenpropathrin with mean value of 0.18 mg/kg .
    The pesticide load ranging from 0.02-3.70 mg/kg
  • Both pre and post-cotton season vegetables
    samples (89 and 92) were containing MRL above
    FAO/WHO codex. While 100 samples exceeds EU
    limits
  • During pre-cotton season only 6 milk samples
    were exceeding codex standard but post-cotton 50
    above codex and EU.
  • Muscular weaknesses, skin burn, coughing nausea
    and vomiting were quite high among cotton
    pickers
  • The pesticide exposure cause disturbance in the
    thyroid and reproductive hormones in agriculture
    workers

60
  • Development of Picking and Pre-cooling Technology
    for Mangoes. Dr. Muslim Abbas Zaidi, ABEI, NARC
  • Achievements
  • Survey on the current practices of picking and
    pre-cooling.
  • Mango picking machine designed and a prototype
    developed in collaboration with Pvt. sector.
  • Machine field tested and evaluated in previous
    mango season for improvements/ modifications. MOU
    signed with Pvt. firm for commercialization.
    (Javed Eng. Gujranwala)

61
  • Intervention for the Management of Mycotoxin in
    Maize and Groundnut Component-I (Mobile Flat-Bed
    Dryer) Dr. Munir Ahmad, CSO, ABEI, (FMI), NARC
  • Achievements
  • Dryer has been tested on ear-corn and further
    modifications are made for gas firing and
    increasing volume. For use of solar energy
    designing process is on.
  • Evaluated the adaptability of dryer for ground
    nut and ear corn and found suitable. Evaluation
    for autumn ear corn at Depalpur / Okara showed
    that moisture content of 4 ton ear corn dropped
    from 28.7 to 19.7 in 10 hours. The cost of
    drying one kg was Rs. 2.9. The cost of drying
    spring corn was Rs. 1.9 /Kg. Dryer can dry
    produce of one acre in a day.
  • Demonstration was carried at Pind Dadan Khan and
    number of farmers participated.
  • Mou has been signed with Malik Engineering
    Rawalpindi for commercialization.

62
  • Development of milking machine for water
    buffaloes and indigenization of milking machine
    for cows. Dr. Jandool Khan, P. Engineer, ABEI,
    NARC
  • Achievements
  • Developed a portable bucket milking machine of
    buffaloes and cows
  • Milking machine designed and fabricated in
    collaboration with Pvt. Firm (Al-Madina- Okara)
  • Tested the performance of machine at Pvt. farm
    Okara and NARC
  • Machine is commercialized by the private sector
    firm

63
NATURAL RESOURCES
64
  • A strategic approach of chemical and biological
    reclamation of salt affected soils in southern
    zone of KPK, Dr. Abdul Rashid, PSO, AZRI-D.I.
    Khan
  • Achievements
  • Three approaches studied at AZRI farm
    physical(MB, soil scraping and deep tillage)
    chemical amendments (gypsum, FYM, press mud and
    humic acid) and salt tolerant crops (kallar
    grass, Dhancha (green manure), guava, falsa. The
    trials at farm station indicated great
    improvements with various combination of these
    approaches/amendments and crop rotations. The
    salts have disappeared and crops in good and
    healthy condition. Test of these approaches to
    start at farmers fields.
  • Leafet in URDU/PUSHTO on technology package for
    farmers in printing. Project reviewed by
    Technical Division in Nov. 2010 and recommended
    for feld demonstration of technology package and
    its transfer to farming community.

65
  • Development of Bio Fertilizer Processing Plant
    Mr. Abdul Waheed Zafar, P. Engineer, ABEI, NARC.
  • Achievements
  • Organic /bio fertilizer processing unit has been
    fabricated and in operation at NARC. The
    processing unit has capacity to manufacture 200
    bags of organic fertilizers per day.
  • Organic fertilizer formulation are as under
  • 3122
  • 3153
  • The raw material used for organic fertilizer are
    farm yard manure, poultry manure, sugarcane press
    mud, rock phosphate, sulphar mud, blood and
    mountains coal.

66
  • Evaluation resource conservation technologies
    for improving water productivity in rice-wheat
    cropping system. Mr. Qurban Hussain, PSO, WRRI,
    NARC
  • Achievements
  • Crop water requirement for wheat and rice crop
    was determined as 383 and 600 mm respectively.
  • Double zero tillage increased water productivity
    by 28 for basmati rice as compared to
    conventional method.
  • Direct seeding increased water productivity by
    18 for Basmati rice as compare to conventional
    one.
  • Saturation and alternate wetting and drying
    irrigation strategies for rice increased water
    productivity by 22 and 19 respectively for
    basmati rice.
  • Bed planting with hybrid rice using mechanized
    process in district Kasur on farmers field saved
    35 water as compared to conventional method
    whereas, in Faisalabad hybrid rice on bed saved
    25 water with 15 increase in yield.

67
  • Water Productivity and Application Efficiency
    Evaluation under Trickle Irrigation System in
    Stress Environment of D.I Khan Engr. Noman Latif,
    D.I.Khan.
  • Achievements
  • Developed a package of technology for utilization
    of vulnerable fresh water in stressed areas of D.
    I. Khan.
  • Different locally fabricated Drip irrigation
    system installed on 21 acres of land in D.I Khan
  • Different locally manufactured drippers were
    tested and recommended for adoption
  • Uniformity of water application was 90 in case
    of micro omitters
  • Survival rate of fruit plant was higher (85) in
    drip irrigation as compared to surface
  • Water saving was 40 as compared to furrow
    irrigation
  • Water productivity was 35-40 higher in drip
    irrigation as compared to furrow irrigation system

68
  • Conservation of native flora of Cholistan through
    rejuvenation technique. Mr. Mumtaz Hussain, SO,
    AZRI, Bahawalpur
  • Achievements
  • The different ecological zones of various strata
    of desert plant species have been identified in
    lesser Cholistan desert of Bahawalpur for future
    seed collection and other activities of the
    project.
  •  Ten thousands stubbles/buds of eight different
    economic geophytes were collected from different
    areas of Cholistan desert and have been
    propagated at Cholistan farm of AZRI, Bahawalpur
    for multiplication.
  •  The seed of different desert trees particularly
    Acacia species were also obtained from Pakistan
    Forest Research Institute, Peshawar to establish
    plants in the nursery of AZRI, Bahawalpur and
    which are ready to provide to the farmers for
    their propogation in Cholistan

69
  • Cultivation of Biofuel Plants on Marginal Lands
    in Pakistan Dr. Rukhsana Anjum, Director AZRI,
    Bahawalpur (Umerkot, D. I. Khan, Karachi)
  • Achievements
  • Procured germplasm of Jatropha curcase from six
    different sources (Australia, India, Thailand,
    Malaysia, Africa ). Raised plants of Pnogamia
    pinnata (Sukh chain) from the locally available
    seed. Seed of Ricinus communis (Castorbean) was
    procured from Ayub Agricultural Research
    Institute, Faisalabad for its successful
    multiplication at AZRI, Bahawalpur. Seed has been
    multiplied a a large scale and is being sold to
    the local farmers.
  • More than 28,000 plants of Jatropha and 8,500
    plants of Castor and Sukh Chane have been
    transplanted on 5 acres at AZRI farm, Bahawalpur
    and 4 acres at Cholistan farm. Success rate is
    remarkably high.
  • The best sowing time (for bio fuel plants)
    identified is March, and September during the
    year.
  • Meanwhile, a very interesting experiment on the
    comparison of growth performance and yield
    potential of caster bean variety (DS-30) .

70
  • Production of Export Quality Honey and
    Establishment of Honey Analysis Laboratory for
    Promotion of Honey Export. Ms. Farida Iftikhar,
    SSO, HBRI, NARC
  • Achievements
  • Organized National Beekeeping Training courses of
    beekeepers for integrated control of American
    foul brood disease, mites, and use of modern
    methods for supplemental feeding to honeybees for
    production of honey free from antibiotics and
    pesticides residues. Trained 60 beekeepers.
  • The honey Analysis Lab. has been established. The
    honey analysis has been started.
  • Parameter tested are pH, total acidity,
    moisture, electrical conductivity, HMF, diastase
    enzyme, sucrose, total sugars and pollen analysis

71
  • Improvement in the production techniques of
    Royal, Jelly, Pollen, Propolis, Beeswax and their
    value added products for livelihood. Mr. Rashid
    Mehmood, SSO, HBRI, NARC.
  • Achievements
  • Newly devised pollen trap technique (Fixed) for
    collection of pollen without disturbing honeybees
    and demonstrated among beekeepers.
  • Developed bottom board pollen trap for beehives.
  • Standardized techniques for the production of
    royal jelly, pollen and successfully produced
    Royal jelly and collected pollen.
  • A limited quantity of bee pollen (Natural food
    supplement) and royal jelly with honey (tonic),
    candle wax sheets are available at Honey Bee
    Research Institute, NARC for sale
  • Initiated Apiculture in Sakardu and training of
    staff.
  • Organized training workshops on Management of
    Honeybees for the production of Royal jelly,
    Pollen, Propolis, Beeswax and their value added
    products.

72
  • Production technology of black cumin M. Qasim,
    SSO, MARC, Juglote, Gilgit
  • Achievements
  • Success fully Black Cumin through seeding and
    introduced in Astore valley of Gilgit Baltistan.
  •  Mountain Agricultural Research Centre (MARC),
    PARC developed production technologies for
    cultivation of Black cumin.
  •  The results of the investigation revealed that
    the Black cumin can be propagated through seeds
    and bulbs. Its excellent aroma fetches attractive
    and higher prices in the market.
  •  The technologies were introduced through
    trainings, filed visits and contacts with the
    farmers and extension departments

73
ANIMAL SCIENCES
74
  • Stair-step heifer development program for
    induction of early puberty. Dr. Imdad Hussain
    Mirza, Director, ASI, NARC
  • Achievements
  • With Stair-step feeding scheme , conception in
    Nili-Ravi buffalo and Sahiwal cattle heifers can
    be achieved in 18-24 month at comparatively
    lower cost without any performance loss
  • with the help of Stair Step Feeding Scheme (6
    phases of 4-2-4-2-4-2 months i.e. low energy diet
    (80 Metabolizable Energy of NRC) for 4 months
    (1st phase), followed by high energy diet (120
    ME of NRC) for 2 months (2nd phase), low energy
    diet for 4 months (3rd phase), high energy diet
    for 2 months (4th phase) and so on) as compared
    to NRC feeding requirements.
  • Average milk yield of Sahiwal heifer reared on
    Stair-step was 26 higher than normal diet
  • SSFS offered a simple, practical and cost
    effective method for raising dairy cattle and
    buffalo heifers.

75
  • Comparison of oestrous synchronization protocols
    to improve fertility in buffalo. Dr. M. Anwar,
    PSO, ASI, NARC
  • Achievements
  • 80 conception rate was achieved in buffaloes
    after estrus synchronization during peak breeding
    season under controlled conditions using Ovsynch
    protocol (GnRH, PGF20)
  • 54 conception rate was achieved in buffaloes
    after synchronizing estrus during peak breeding
    season under field conditions.
  • PGF 20 worked well to induce fruitful heat in
    buffalo during low breeding season (Feb-Jun)
  • More valuable finding was that 80 buffaloes
    showed estrus in low breeding season (summer)
    after the application of hormonal
    treatment/device and 40 treated animals became
    pregnant. In this way season ability of breeding
    was partially overcome in buffaloes.
  • So these treatments could be used to popularize
    artificial insemination in buffaloes.

76
  • Cryopreservation and evaluation of buffalo and
    goat semen. Dr. Abid Mehmood, PSO, ASI, NARC
  • Achievements
  • A simple milk based extender, which is easy to
    use, was standardized for the cryopreservation of
    buffalo semens.
  • Addition of antioxidant (Glutathionine) improved
    post-thaw ability of buffalo bull semen.
  • Kidding with artificial insemination (A.I) in
    heat synchronization does was achieved
    successfully for first time in Pakistan
  • A cost effective and short duration heat
    synchronization protocol with cloprosterol
    injection (synthetic prostaglandin) for fix time
    A.I. in goats was standardized

77
  • Study on Biology, Captive breeding aspects of
    indigenous endangered wild animals and birds Mr.
    Saleem Zahid, PSO, Poultry, ASI, NARC
  • Achievements
  • Facilities for breeding of endangered wild
    animals and birds were established at ASI, NARC.
    Currently 10 wild animals and 18 wild birds are
    maintained at this facility.
  • Breeding of Hog deer, Black Buck, Jungle fowl,
    Pheasants and Peafowl under captivity was
    achieved successfully for their conservation and
    further propagation.
  • Breeding results and data on captive breeding
    have been compiled to share with students,
    researchers, farmers and wildlife lovers.

78
  • Characterization of avian influenza FMD viruses
    and development of immunogenic vaccines. Dr.
    Khalid Naeem, CSI, NARC
  • Achievements
  • Bio-safe laboratory were established at NARC
  • Established cell culture (BHK-21) set up for
    growth of FMD viruses. The cell line was also
    shared with FMD research center, VRI, lahore for
    propagation of FMDV, which was replaced their 30
    years old cell line stock
  • Sequencing of 25 isolates of Avian influenza and
    2 FMD viruses was carried out and the sequence
    data was submitted to gene bank.
  • RT-PCR and Immuno-capture ELISA for FMD
    diagnosis was standardized.
  • A total of 16 isolates of FMDV serotype (A,O,
    Asia 1) were propagated successfully in BHK-21
    and lyophilized for future use in developing new
    FMD vaccines

79
  • Diagnosis and Control of Parasitic and Microbial
    Infestation in Exotic/ Indigenous Carp Cultured
    in Fish Farm of Punjab. Dr. Muhammad Afzal, SSO,
    Aquaculture Fishries Program, NARC
  • Achievements
  • Thirty one fish farm and hatcheries were visited
    in distt Faisalabd, Jhang and Toba Tek singh
  • Fish samples were collected randomly from
    selected farms for the disease diagnosis . The
    fish samples included the major and chinese carb
  • Sampled fish were examined externally for
    parasites and the fish samples were also brought
    back to NARC for bacterial studies
  • Lernea, Argulus, Dropsy, Gillrot, Finrot were
    found in 21 fish farms. The prevalence of disease
    in visited farms was found to be almost 69.
  • Saprolegniasis was also found in Satiana. Catla
    catla, Labeo rohita and H. molitrix were infected
    with bacterial disease, fin rot.
  • Lernea, Argulus, Dropsy, Gillrot, Finrot of fish
    in pond culture was treated with bath /treatment
    (KMNO4/Antibiotics and DOB)

80
  • Feed formulation and disease diagnostics studies
    of trout fish in northern areas, Mr. Muhammad
    Aziz, SSO, MARC, Gilgit
  • Achievements
  • Developed cost efficient and balance feed for
    raising fish . This increased the average growth
    rate 15-25 gm/month. Reduced feed cost up to 60
  • Seven bacterial diseases of trout fish were
    identified and successfully treated with 90
    recovery rate.
  • Reduced nutritional disease i.e. degeneration of
    liver, inflammation of intestine, with increase
    of vitamins in adult stages.
  • The research findings have been communicated
    through personal contacts to the local trout
    farmers and extension workers of G-B Fisheries
    Department.

81
SOCIAL SCIENCES
82
  • Political Economy of Sugar Industry in Pakistan,
    Dr. Ali Muhammad Khushk, PSO, TTI, ARI, Tandojam
  • Achievements
  • Previous sugar policies reviewed through
    literature review
  • Comprehensive data of all stake holders collected
    through interviews and analysed
  • A report on all actors involved in sugar industry
    from farmers to sugar mills with their weaknesses
    and strengths is prepared and several policy
    recommendations of all levels are made.
  • Bi-products and other sources of sugar/sweeteners
    identified and proposed to reduce pressure on
    cane.
  • The report and recommendations have been sent to
    MINFA, APCOM, TCP, Pak Sugar Mills Owner
    Association and growers association for their
    consideration/implementation.

83
  • Assessment of harvest and post-harvest losses in
    selected fruits in Pakistan, Mr. Mazher Abbas,
    SSO, TTI (PARC) AARI, Faisalabad
  • Achievements
  • Heavy losses reported in Mango (11.5) and citrus
    (8), with economic value of loss of Rs. 3735/t
    and Rs. 1842/t.
  • The losses estimated for mango, citrus and dates
    for year 2008-09, was Rs. 46.0 billion in
    monetary term.
  • Up-scaling capacity of farmers contractors,
    businessmen recommended.

84
  • Research Strategies for Agricultural Growth and
    Poverty Reduction Dr. Umar Farooq, CSO, SSD, PARC
  • Achievements
  • Poverty alleviation coefficient estimated for
    various factors contributing to poverty
    alleviation, which revealed a long term
    relationship between agriculture sector and
    poverty reduction.
  • Major crop rotations addressed on the water audit
    basis to save on water cost as well as water
    saving.
  • Zonal research system on the farming system
    research is found better option for research
    priorities as well as on product development with
    value addition. More profitable crops and
    products to be prioritised for improvement of
    research system.
  • More research system related reforms and options
    suggested.

85
  • An analysis of Food Consumption Diversification
    in Pakistan, Muhammad Ishaq, SSO, TTI, Ternab,
    Peshawar
  • Achievements
  • Consumption trends in Pakistan has shown
    considerable variation over time, there is
    considerable downward trend in consumption of
    wheat and pulses, but upward in other
    commodities.
  • Shift is from low value to high value food
    products.
  • The demand for all feed products is price
    inelastic.
  • Policy implication for planning to make available
    products ahead of time.

86
  • An Analysis of the Adoptation and Impact of
    Cultivation of new cotton varieties in Pakistan
    Mr. Muhammad Ibrahim Lashari, TTI, Tandojam,
    Sindh
  • Achievements
  • Survey completed comprising 440 Bt cotton growers
    using imported identification immune-strips.
  • First draft report on growers has been prepared
    and submitted. Results shared with higher
    authorities for taking necessary
    regulatory/policy actions.
  • 47.5 cotton area planted Bt Cotton in 2008,
    which increased to 63 in 2009.
  • Results indicate 23 GM/Bt cotton varieties were
    grown and 5-46 samples were detected as fake Bt
    cotton with Punjab 15, Sindh 17 and Baluchistan
    07.
  • The fake Bt cotton cost Rs. 345 million to
    farmers in 2009.

87
Ongoing Sub-Research Projects of RADP
88
CROP SCIENCES
89
  • Genetic Manipulation for Induction of Resistance
    against Fungal Disease and Potato seed Production
    Dr. Iqbal Hussain, SO, PBP, NARC
  • Achievements
  • Production of 40,000 virus free in vitro plants
    of four potato varieties (Kuroda, Desiree, Santey
    Hermes and Lady Rosetta).
  • Production of six ton virus free potato seed.
  • Virus free seed was disseminated among
    progressive farmers for further multiplication.
  • A part of seed is planted at Kaghan for further
    multiplication.
  • Collaborative seed multiplication is under way
    with Hazara Research Station Abottabad and Deptt
    of Agriculture Gilgit Baltistan.
  • Production transgenic potato having resistance
    against fungal diseases.
  • Human resource training in potato tissue culture
    from Northern areas and Ph.D/M. Phil. students.
  • Published research achievements in
    local/international journals.

90
  • Making NARC campus Rodent Free through
    operational research, Shahid Munir, SSO,
    VPMP/IPEP/NARC
  • Achievements
  • RODENT CONTROL AT NARC FARM
  • Total cultivated area of NARC was divided into 12
    zones where rodent control operation was carried
    out.
  • Infested areas especially wheat, fodder,
    sugarcane, vegetable fruit orchards were
    targeted to manage the rodent population in the
    first phase.
  • Control strategies comprised of application of
    fumigants, acute and chronic rodenticides.
  • A total of 18,611 burrows were treated in three
    steps. In first treatment fumigation with
    aluminium phosphide was carried out followed by
    application of different formulations of acute
    and chronic rodenticides.
  • Rodenticide baits were applied in PVC bait
    station and using underground baiting technique.
  • Overall 57.81 Kg Aluminium phosphide tablets,
    47.85 Kg Zinc phosphide grain bait and 117 Kg
    Coumatetralyl grain bait was used. Overall 80 to
    90 reduction in burrow activity was estimated by
    counting the pre and post-treatment number of
    burrows.
  • One day hands on training in rodent management in
    field crops was imparted to 35 field staff
    personnels and DPLS of different commodity
    programmes of NARC.
  • RODENT CONTROL IN STRUCTURES
  • A total of 423 PVC bait stations were installed
    in Offices, labs, Stores, and residential areas.
    Overall 47.9 Kg Coumatetralyl pellet bait was
    applied in PVC bait stations. No further damage
    was reported from the treated area.

91
  • Genetic diversity analysis of brassica oilseeds
    and adaptability testing of elite lines at
    different ecologies (follow up project) Abdul
    Ghafoor, PSO, IABGR, NARC
  • Achievements
  • Two hundred and forty two accessions of brassica
    germplasm were regenerated during 2012 for
    conservation and distribution to user
    communities. In addition, one hundred and ten
    accessions of brassica were collected from
    Bahawalpur and Mingora, Swat. During the project
    period (2 years), 490 accessions of brassica
    oilseeds were added in the genebank after
    regeneration under field conditions at NARC.
  • Thirteen promising genotypes (24866, 27388,
    27397, 27398, 27406, 27410, 27425, 27435, 27440,
    27443, 27444, 27445, 27450) of brassica from
    exotic origin were identified on the basis of
    their superior performance and these were planted
    under six locations to select the best one/s. The
    data is yet to be compiled. In addition to
    multilocational trials, 100 accessions of
    brassica were planted under field condition at
    NARC for characterization and evaluation purpose,
    and among these thirty eight accessions did not
    germinate or were damaged badly with hail storm,
    hence the data were recorded for sixty two
    accessions that will be presented in the report.
  • Seed proteins profiling of 200 accessions were
    conducted that indicated low genetic diversity
    among accessions within one species.
    Fingerprinting of cultivars was made on the basis
    of SSR markers. One hundred and eight SSR primer
    pairs were screened using two improved cultivars
    of Brassica carinata and most informative markers
    were identified for further use.

92
  • Achievements
  • DNA profiling of 100 accessions of Brassica was
    carried out using 52 SSR markers for diversity
    analysis.The SSR markers analysis will help us in
    variety protection, plant breeder rights and
    marker assisted breeding.
  • More than 500 accessions were analyzed for
    erucic acid and glucosinolates, and nine
    accessions (27380, 27382, 27391, 27392, 27394,
    27438, 27443, 26147, 26321) exhibited low erucic
    acid and glucosinolates. The seed of these
    accessions will be multiplied and will be shared
    with the researchers working on brassica
    improvement.
  • A promising line (26187) of Eruca sativa
    (Taramira) having yield potential of more than
    1200 kg/ha has been identified and it was planted
    at four location during 2012-13 and excelled as
    compared to the local cultivar.
  • One M.Phil and two Ph.D. students have already
    completed their dissertation research, while
    research work of three students is underway at
    the moment.

93
Determination of the severity of HLB and CTV in
citrus growing areas of Punjab and KPK, Dr.
Shahid Hameed, SSO, IPEP, NARC Achievements MONI
TORING OF CITRUS TRISTEZA VIRUS (CTV) IN
PUNJAB  Major growing areas of Punjab were
surveyed and 510 random citrus leaf samples were
collected. The areas surveyed include Sargodah,
Toba Tak Singh, and Faisalabad. The samples were
tested through DAS-ELISA against CTV. Incidence
range in the province was 10-40. Citrus Tristeza
Virus (CTV) diseases was prevalent in all area
surveyed. MONITORING OF CTV IN KHYBER
PAKHTUNKHAWA PROVINCE  Citrus growing areas of
Khyber Pakhtunkhawa province were surveyed and
300 random citrus leaf samples were collecteted.
The areas surveyed include Peshawar, Nowshera,
Mardan, Swabi, Swat, Malakand Agency and Dir. The
samples collected were tested against Citrus
Tristeza Virus (CTV) diseases through DAS-ELISA
for detection and identification in accordance to
find the incidence and prevalence of this
disease. Forty citrus samples were tested through
ELISA against Citrus Triteza Virus (CTV). The
result showed that all locations were found
infected with CTV with different infection rate.
Percent incidence of CTV in Peshawar,
94
Cont. Nowshera, Mardan, Swabi, Swat, Dir and
Malakand Agency were 50, 45, 28, 20, 16, 44
and 20 respectively. An average incidence of
43.33 was recorded in the KPK province.  GENETIC
DIVERSITY AMONG CTV ISOLATES Six Citrus Tristeza
virus (CTV) ELISA positive were amplified through
PCR and an expected product size of ca. 600 bp
was obtained. CITRUS GREENING The citrus plants
with HLB symptoms showed pale yellowing with
mosaic pattern or variegated type of chlorosis
and reduce in size. Based on these symptoms 35
samples of NARC citrus field were collected and
analyzed through PCR using five different methods
i.e. DNA zol, Plant Phine PcR Direct kit,,
Genomic DNA purification kit, CTAB method and DNA
NEASY Kit. The DNA efficacy was analyzed through
gel electrophoreses and was found maximum. Later
PCR was used to analyze by using three sets of
primers viz. A2/55, Oi1/Oi2C, and General Primers
for HLB. The first set Oi1/Oi2c is 16rDNA based
primer which is specific for detection of
Ca-L-asiaticus and Ca-L-Africanus yielding the
1160pb PCR product after amplification. Although
the infected trees showed clear symptoms but PCR
based detection has not yet achieved and is in
process.
95
  • Management of Viruses infecting Garlic in
    Pakistan, Talat Shaheen Gilani, SSO,IPEP, NARC
  • Achievements
  • Survey of four locations of Punjab Khanewal,
    Gujranwala ,Sialkot and Kasur was carried out
    and 290 samples were collected from different
    farmer fields and age infection recorded was
    33.44 through DAS ELISA against OYDV.
  • Survey of eight locations of Khyberpukhtunkhawa
    was carried out and 400 samples from farmer
    fields were collected. DAS ELISA results of
    samples showed that OYDV was prevalent at all
    locations with maximum 90 at Swabi and minimum
    of 20 at Malakand and Nowshera.
  •  Identification and indexing of garlic germplasm
    was carried out at NARC field for seven
    varieties of garlic cv Iranian, Italian, Chinese,
    NARC-09,MJ-84, JS-1 and Desi to find out the
    tolerant /resistant varieties.The age infection
    of OYDV was decreased due to the management
    practice i-e the sowing of virus free Elisa
    tested cloves in the field.
  • All plants of seven varieties tested through DAS
    ELISA showed different degrees of infection.The
    decrease of infection was due to the good
    management practices adopted durin experiment.
  •  OYDV and Leek Yellow Stripe Virus (LYSV) were
    recorded for the first time in Pakistan.

96
  • Establishment of Botanical Garden for Cultivation
    of wild plants of Pakistan to introduce
    non-conventional crops in cultivation for value
    addition, Dr. Rubina Akhtar, PSO, IABGR, NARC
  • Achievements
  • Land preparation, weed clearing, land levelling,
    irrigation channels done. Landscape design
    prepared. Seed sowing for spring planting done.
  • Bulbs and cuttings grown for spring season
    according to the requirement of plant species.

97
  • Extraction and analysis of essential oil from
    rose, jasmine and aromatic herbs, Dr. Muhammad
    Naeem Ullah, PSO, IABGR, NARC
  • Achievements
  • Clonal repository of medicinal plants including
    mint, basil, thyme, lavender, rosemary, oregano
    and other important plants is being maintained at
    Institute of agricultural Biotechnology Genetic
    Resources, NARC.
  • Mint germplasm was characterized for major
    polyphenols. Caffeic acid was present in highest
    amount (315.4 mg/g DW) in catnip mint followed by
    white mint (314.8 mg/g DW). Cool mint (M.
    spicata) was having highest amount of rosmarinic
    acid (298.2 mg/g DW). Ferrulic acid was also
    present in considerably high quantity suggesting
    the mint as potential source of dietary phenols.
  • Essential oil of 18 mint local and exotic
    accessions was analyzed for chemical
    constituents. A wide array of chemical compounds
    was detected. Highest menthol contents were
    detected in locally collected white and purple
    flower mint. Mentha longifolia. Nana Asavi
    introduced from Saudi Arabi
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