Biosensors Initiative Collage

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BiosensorsInitiative Collage
Ryszard Lec Professor of Biomedical Electrical
Engineering

• Drexel University
• Philadelphia, PA

www.biomed.drexel.edu
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Nano-Biosensor Science, Engineering and
Technology The Challenges

• Attain a fundamental understanding of nanoscale
  biosensing phenomena.
• Design and fabricate biologically active sensing
  interfaces DNA, proteins, cells, tissues, other.
• Design and fabricate solid-state based transducer
  structures capable of simultaneous detection of
  multiple biological substances and processes
  biosensor chips, biosensor arrays, other.
• Novel theoretical and experimental tools for a
  rapid development of the Nano-Biosensor
  technology.
• Integration of biological, physical (mechanical,
  optical, acoustic) and electronic components into
  multifunctional biosensor systems novel
  immobilization techniques solid-state transducer
  nano/microfabrication technologies microfluidic
  systems IC circuits for signal conditioning and
  processing smart biosensors and biosensor
  systems.

Endothelial Cell Proliferation
Sedimentation
Adhesion
Proliferation
Sedimentation, adhesion, and proliferation of
Endothelial Cell Proliferation
Deposition of super collagen on the gold surface
in 0.1 mol of HCl solution
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Nano-Biosensor Science, Engineering and
TechnologyObjective Development of
Piezoelectric Nano-Biosensor Technology
PlatformImportant Features
PNBS Frequency 1 MHz 100 MHz 1 GHz
980 nm 98 nm 28 nm
PNBS Frequency 500 MHz
Penetration Depth 37 nm

• Multidomain Piezoelectric Sensing Mechanisms
  mass,
• viscosity, elasticity, electric conductivity, and
  dielectric
• constants.
• Real-time Piezoelectric Monitoring of Interfacial
• Biological Phenomena the depth of monitoring
  ranges
• from a single to hundreds nanometers with the
  time resolution
• of milliseconds.
• Piezoelectric Biotransducer Technology IC
  compatible,
• MEMS/NEMS sensing and actuating
  multiple-sensing-
• wave transducers, piezo-bio-chips and arrays,
  other.
• Bio-Piezo-Interfaces design and synthesis of
  surfaces at the
• atomic level to produce sensing interfaces with
  desired
• properties and functions.
• Integrated Electronic Signal Processing and
  Display
• Technologies fast, miniature, inexpensive,
  reliable.

Shear-Mode Piezo-Biosensor (Fundamental)
Shear-Mode Piezo-Biosensor (Harmonics)
100 MHz
1 GHz
10 MHz
Piezo-Bio-Array
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Nano-Biosensor Science, Engineering and
TechnologyObjective Novel Applications of
Piezoelectric Nano-Biosensor Technology

• DNA sensors/chips genetic screening and
  diseases, drug testing, environmental monitoring,
  biowarfare, bioterrorism, other.
• Immunosensors HIV, hepatitis, other viral
  diseases, drug testing, environmental monitoring,
  biowarfare, bioterrorism, other.
•  
• Cell-based sensors functional sensors, drug
  testing, environmental monitoring, biowarfare,
  bioterrorism, other.
• Point-of-care sensors blood, urine,
  electrolytes, gases, steroids, drugs, hormones,
  proteins, other.
• Bacteria sensors (E-coli, streptococcus, other)
  food industry, medicine, environmental, other.
• Enzyme sensors diabetics, drug testing, other.
• Market clinical diagnostic, environmental
  monitoring, biotechnology, pharmaceutical
  industry, food analysis, cosmetic industry,
  other.
• Immunosensors about 1 billion annually
• DNA probes about 1.5 billion annually

Integrated Laboratory System for Testing and
Calibration of Piezoelectric Biosensors
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Nano-Biosensor Research Laboratory
Project Title Sensing Principles of
Piezoelectric Nano-Biochemical Sensors (PNBS)
Michael Francois (Ph.D), Sun Jong Kwoun (MS
Student) Advisors Dr. Ryszard M. Lec, Dr. Kambiz
Pourrezaei
PNBS Frequency 1 MHz 100 MHz 1 GHz
980 nm 98 nm 28 nm
PNBS Frequency 500 MHz
37 nm
Fig. 1. PNBS operating at the fundamental
frequency
Fig. 2. PNBS operating at the harmonic
frequencies
100 MHz
10 MHz
1 GHz
Penetration Depth nanometers
Fig. 3. Probing depth of the PNBS as a function
of frequency
Fig. 4. A PNBS Biochip
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Nano-Biosensor Research Laboratory
Project Title A Novel Polymer Nanofiber
Interface for Biochemical Sensor
Applications
Sun Jong Kwoun (MS Student) Advisors Dr. Ryszard
M. Lec (Biomed.), Dr. Frank K. Ko (Material Eng.
Dept.)
Fig. 2. SEM picture of the nanofiber film at
6000X magnifications.
Fig. 1. Photograph of the TSM sensor with
deposited nanofiber film.
Fig. 3. Endothelial cell attached to the
nanofiber matrix.
NF Sensor
Air
Fig. 4. The S21 amplitude of the nanofiber
deposited sensors in air as a function of
frequency for five different deposition time
(corresponding to different NF film thickness).
Fig. 3. Cell growth on nano fibrous matrix
(after 1 day).
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Nano-Biosensor Research Laboratory
Project Title Piezoelectric Biosensor for
Monitoring the Interaction of a single
cell with solid surfaces Endothelial Cell on
Gold Surface
Qiliang Zhang (Ph.D student) Advisors Dr.
Ryszard M. Lec (Biomed), Dr. Kambiz Pourrezaei
(ECE)
Nano-Microparticle-Cell on the surface of the
sensor
High Frequency Excitation
Piezoelectric Sensor
Piezoelectric Sensor
Piezoelectric Sensor
Nano-Microparticle-Cell m mass k effective
elasticity representing interfacial bonding
energy r dissipative losses
Amplitude
?f
k
r
Reference Sensor Response
Nano-Microparticle-Cell on the surface of the
sensor
Sensor Response with a Nanoparticle
Piezoelectric Sensor M mass k elasticity r
dissipative losses
Piezoelectric Sensor
R
10.10000
Frequency (MHz)
10.00000
Equivalent Electromechanical Circuit
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Nano-Biosensor Research LaboratoryProject
Title Piezoelectric Nano-Biosensor for
Monitoring Interfacial Cell Processes
Endothelial Cell Properties such as
sedimentation, adhesion, proliferation and
fixation Students Vadivel Devaraju, (PhD)
and Joe Sorial, (MS)Faculty Dr. Ryszard M. Lec
and Dr. Kenneth Barbee
Fixation
Sedimentation
5MHz
Attachment
Proliferation
Fixation
15MHz
Figure 1. (a) Sedimentation, adhesion and
proliferation profile of endothelial cells as a
function of time measured
using 25 MHz piezoelectric resonant
sensor the caption for the second picture
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Nano-Biosensor Research LaboratoryProject
Title Piezoelectric Nano-Biosensor for
Monitoring Interfacial Protein Processes
Student Vadivel Devaraju, (PhD)
Faculty Dr. Ryszard M. Lec and
Dr. Andrew Fertala
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School of Biomedical Engineering,Science
Health Systems
WWW.BIOMED.DREXEL.EDU

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