BEL7402

1
S1
BEL7402
Huh7
C Tet Rapa C Tet Rapa
LC3-I
LC3-II
GAPDH
Figure S1 Detecting Tetrandrine-induced
autophagy in Huh7 and BEL7402 cell lines.
Rapamycin treatment was used as a positive
control. These cells were treated with 5µM
Tetrandrine or 100nM rapamycin for 24 hours. Cell
lysis were subjected to Western blot analysis to
detect LC3 expression level.
2
BEL7402
HepG2
Huh7
S2
Figure S2 Acridine orange staining. (top) Huh7,
BEL7402 and HepG2 cells were incubated with 5
µmol/L Tetrandrine for designated time points,
and then stained with acridine orange (1 µg/mL),
and examined by a fluorescence microscope.
Magnification, 40. (bottom) The ratio of cells
containing acidic vesicular organelles (AVOs).
Columns, mean percentage of autophagy cells
bars, SD. At least 300 cells from each treatment
group were observed.
3
S3
Huh7
Tet - - 3-MA - -
LC3 II
GAPDH
Figure S3 Huh7 cells were pretreated with 2 mM
3-MA for 1 h, and then with or without 5 µM
Tetrandrine for 24 hours, and the expression
levels of LC3 were detected.
4
S4
Figure S4 Huh7 cells were treated with 5 µM
Tetrandrine for indicated time. Cell lysis were
collacted and subjected to Caspase 3 activity
analysis. Level of untreated Huh7s caspase 3
activity was set as 100.
5
S5
Figure S5 Nude mice bearing Huh7 cell tumor
xenografts were randomly distributed into two
groups (n7) and administered either the vehicle
only (Vehicle) 25 or 50 mg/kg body weight
tetrandrine by gavage for 20 days. The body
weight of nude mice were measured every several
days.