Gas Chromatography, GC

1
? 20 ? ? ? ? ? ?

• Gas Chromatography, GC

2
?20? ?????
????
3
?20? ?????
20.1 ?????
20.1 ?????
?????????????? ???????????
4
?20? ?????
20.5 ????????
????????
5
?20? ?????
20.7 ?????????

• ?????????

• ????/???? GC/MS
• GC/MS?????????????????????????
• 2. ????/????????? GC/IR
• ?GC????????IR????????????,????????????

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?20? ?????
20.7 ?????????

• GC/MS???

(1)??????? (2)????? (3)??? (4)?????????????? (5)?
?????
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?20? ?????
20.7 ?????????
?20-16 ???????? ???????(total ionic
chromatogram,TIC)??????????????????????????????
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?20? ?????
20.7 ?????????
C14-C22???????TIC?M-CH3?MC (p510) ????????????
?,?????????,?????????,???????????????????,?????,??
?????,???????(mass chromatogram,MC),??????????
9
?20? ?????
20.7 ?????????
?20-18 ???????????? ?GC/MC???,???????????????????
??????????????,??????????????,??????????(mass
fragmentogram,MF),??????????
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? 21 ? ???????

• High-Performance Liquid Chromatography,HPLC

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?21? ???????
HPLC??
(1)???? (2)???? ??HPLC
???C18?C8???
????-????-?? (3)??????(IEC) (4)??????(SEC) (5)???
?(AC)
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?21? ???????
HPLC??
??????????????????????
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? 22 ? ??????

• Capillary Electrophoresis, CE

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?22? ??????
CE v.s. ??
???????? ???? ????? ???????
????
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?22? ??????
CE v.s. ????
(1)????? (2)????? (3)????? (4)?????? (5)????? ????
?????
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?22? ??????
22.1 ????????
??????
CE???????????????????
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?22? ??????
22.1 ????????
????????
?? ????????,????????? ???????? ??
????????,?????????? ??????????????
?????????????????
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?22? ??????
22.1 ????????
??? v.s. HPLC??
????HPLC???(?)????????(?)
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?22? ??????
22.1 ????????
?22-3 ????????????????? (a)???????
(b)?????????????????,?????0 (c)????????????????
?,??????
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?22? ??????
22.1 ????????

• ??????

• ?????,???????
• ????????????5-7?
• ????????????????????????,??????,??????????????????
  ????
• ????????????????
• ???????????,???????
• ??????????????

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?22? ??????
22.1 ????????

• ????????
• ????????
• ?????????? Zeta??
• ?????pH
• ?????
• ???? ??

?-????
?????
22
????
?22? ??????
22.2 ????
1 ??????? CZE 2 ????????? MEKC 3 ??????? CGF 4
??????? CITP 5 ??????? CIEF 6 ?????? CEC
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?22? ??????
22.3 ?????
????