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International Hands-on workshop on genotoxiciy monitoring bioassays for environmental pollution

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International Hands-on workshop on genotoxiciy monitoring bioassays for environmental pollution. University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland – PowerPoint PPT presentation

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Title: International Hands-on workshop on genotoxiciy monitoring bioassays for environmental pollution


1
International Hands-on workshop on genotoxiciy
monitoring bioassays for environmental pollution
  • University of Silesia, Jagiellonska 28,
  • 40-032 Katowice, Poland
  • Directed by
  • Prof. Jolanta Maluszynska, Department of Plant
    Anatomy and Cytology, University of Silesia,
    Katowice, Poland
  • Prof. Siegfried Knasmueller, Institute for Cancer
    Research, The Medical University of Vienna,
    Vienna, Austria
  • Prof. Michael Plewa, University of Illinois,
    Champaign-Urbana, IL, USA

2
International workshop on genotoxicity monitoring
bioassays for environmental pollution
Group photo of the participants
3
Prof. Knasmueller (left) and Prof. Maluszynska
(right) discuss workshop plan
4
Prof. Jolanta Maluszynska giving instructions at
the workshop, demonstrating Fluorescent in situ
hybridization (FISH) assay (photomicrograph was
not available)
5
Instructor of the workshop giving a lecture to
the participants.
6
Prof. Michael Plewa of the University of Illinois
demonstrating the slide preparation for Comet
test for clastogens
7
Photomicrograph of a comet tail as the result of
DNA damage by clastogens
8
Mr. Bernhard Majer of the medical University of
Vienna prepares Allium roots and Tradescantia
cuttings for chromosome aberration and
micronucleus assays .
9
Tradescantia plants bearing young inflorescences
provide the test material for both Tradescantia-
Stamen-Hair (Trad-SHM) and Tradescantia-Micronucle
us (Trad-MCN) assays
10
Photomicrograph of Sister-Chromatid-Exchange
(SCE) in Tradescantia root tip cell
Photomicrograph of Sister-Chromatid-Exchange
(SCE) in Tradescantia root tip cell cell.
11
Dissection of flower buds in preparation of
Trad-MCN slides from the meiotic microsporocytes
12
Preparing stamen hairs of Tradescantia flower
under a dissecting microscope for scoring
frequencies of pink mutation events in the
Trad-SHM assay.
13
MCN
MCN
Photomicrograph showing micronuclei (MCN) among
the resting stage root tip cells in the
subsequent generation of treated nuclei
14
MCN
MCN
MCN
Photomicrograph of the tetrad stage of
microsporocyte of Tradescantia showing
micronuclei (MCN) among the synchronized tetrads
in Trad-MCN assay
15
Scoring pink mutation events under the dissecting
microscope in the Tad-SHM assay
16
Tradescantia flowers ready for scoring pink
mutation events after a 16- minute refrigeration
in the Trad-SHM assay
17
blue
pink
blue
pink
Photomicrograph of orderly aligned stamen hairs
showing 3 pink mutation events in this field of
view in the Trad-SHM assay
18
Collecting data for frequencies of mutation and
micronueli in treated and control groups of
Trad-SHM and Trad-MCN assays
19
DATA analysis using computer software and print
out for report write up
20
After a days hard work, participants took a tour
around town
21
Relaxing and shopping in the Mall in the city of
Katowice, Poland
22
Lets have some coffee and snack
23
A walk outside to have some fresh air
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