International Hands-on workshop on genotoxiciy monitoring bioassays for environmental pollution

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International Hands-on workshop on genotoxiciy
monitoring bioassays for environmental pollution

• University of Silesia, Jagiellonska 28,
• 40-032 Katowice, Poland
• Directed by
• Prof. Jolanta Maluszynska, Department of Plant
  Anatomy and Cytology, University of Silesia,
  Katowice, Poland
• Prof. Siegfried Knasmueller, Institute for Cancer
  Research, The Medical University of Vienna,
  Vienna, Austria
• Prof. Michael Plewa, University of Illinois,
  Champaign-Urbana, IL, USA

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International workshop on genotoxicity monitoring
bioassays for environmental pollution
Group photo of the participants
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Prof. Knasmueller (left) and Prof. Maluszynska
(right) discuss workshop plan
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Prof. Jolanta Maluszynska giving instructions at
the workshop, demonstrating Fluorescent in situ
hybridization (FISH) assay (photomicrograph was
not available)
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Instructor of the workshop giving a lecture to
the participants.
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Prof. Michael Plewa of the University of Illinois
demonstrating the slide preparation for Comet
test for clastogens
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Photomicrograph of a comet tail as the result of
DNA damage by clastogens
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Mr. Bernhard Majer of the medical University of
Vienna prepares Allium roots and Tradescantia
cuttings for chromosome aberration and
micronucleus assays .
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Tradescantia plants bearing young inflorescences
provide the test material for both Tradescantia-
Stamen-Hair (Trad-SHM) and Tradescantia-Micronucle
us (Trad-MCN) assays
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Photomicrograph of Sister-Chromatid-Exchange
(SCE) in Tradescantia root tip cell
Photomicrograph of Sister-Chromatid-Exchange
(SCE) in Tradescantia root tip cell cell.
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Dissection of flower buds in preparation of
Trad-MCN slides from the meiotic microsporocytes
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Preparing stamen hairs of Tradescantia flower
under a dissecting microscope for scoring
frequencies of pink mutation events in the
Trad-SHM assay.
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MCN
MCN
Photomicrograph showing micronuclei (MCN) among
the resting stage root tip cells in the
subsequent generation of treated nuclei
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MCN
MCN
MCN
Photomicrograph of the tetrad stage of
microsporocyte of Tradescantia showing
micronuclei (MCN) among the synchronized tetrads
in Trad-MCN assay
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Scoring pink mutation events under the dissecting
microscope in the Tad-SHM assay
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Tradescantia flowers ready for scoring pink
mutation events after a 16- minute refrigeration
in the Trad-SHM assay
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blue
pink
blue
pink
Photomicrograph of orderly aligned stamen hairs
showing 3 pink mutation events in this field of
view in the Trad-SHM assay
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Collecting data for frequencies of mutation and
micronueli in treated and control groups of
Trad-SHM and Trad-MCN assays
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DATA analysis using computer software and print
out for report write up
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After a days hard work, participants took a tour
around town
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Relaxing and shopping in the Mall in the city of
Katowice, Poland
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Lets have some coffee and snack
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A walk outside to have some fresh air