Biological Laboratory Safety - PowerPoint PPT Presentation

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Biological Laboratory Safety

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Title: Biological Laboratory Safety


1
Biological Laboratory Safety
  • Additional Information

2
Where to start
  • Before looking at this presentation, ensure that
    you have watched the one on General Laboratory
    Safety

3
What are the additional hazards from using
Biological Materials?
  • Infectious agents
  • Toxins
  • Prions
  • Bacteria
  • Viruses

4
What legislation applies?
  • The main pieces of legislation are
  • Genetically Modified Organisms (Contained Use)
    Regulations 2000
  • Control of Substances Hazardous to Health Regs
    2004
  • Each will be summarised separately

5
Control of Substances Hazardous to Health Regs
2004
  • Four Hazard Groups
  • No hazard group 4 (highest risk) work in
    Sheffield
  • Work on hazard group 3 requires special training
    and Level 3 containment facilities
  • Risk assessments to be submitted to local LGMSC
    for scrutiny for all GM work and work with
    dangerous pathogens
  • Appropriate waste segregation required

6
Genetically Modified Organisms (Contained Use)
Regulations 2000
  • All work to be risk assessed using the HSE risk
    assessment form and be submitted to the Local
    Genetic Modification Safety Committee before work
    starts
  • Approval notification procedures
  • Workers to submit health declarations to
    Occupational Health
  • Appropriate waste segregation

7
Protective Clothing
  • An appropriate laboratory coat must always be
    worn, fastened up and covering all normal
    clothing
  • Lab coats must be decontaminated before
    laundering
  • Appropriate eye protection must be worn
  • Gloves must be worn for work and must be removed
    when handling taps, note books etc and before
    leaving the laboratory

8
Blood bodily fluids
  • Use screened animal blood, rather than human
    blood if possible
  • Failing this use only screened human blood
  • All blood and bodily fluids should be considered
    potentially infectious and treated with due care
  • Injuries involving body fluids must be reported
    to your supervisor IMMEDIATELY

9
Spillages
  • Report
  • Wear protective clothing
  • Clean up
  • Decontaminate ensure that you always have
    material available to do so
  • Ensure waste is disposed of properly
  • You spill you clean up!

10
Laminar Flow Cabinets
  • Laminar flow cabinets are designed to provide a
    clean-air environment to protect the work
  • They DO NOT provide protection for the operator

11
Biological Safety Cabinets
  • Class 1
  • Some protection to user environment
  • No protection to work
  • Class 2
  • Good protection to user, the environment and the
    work
  • Class 3
  • Total enclosure
  • You must choose the correct safety cabinet for
    the job you are doing. Seek advice from your
    Biological Safety Officer

12
Autoclaves
  • Potentially dangerous equipment
  • Require regular maintenance and an annual
    statutory examination
  • Discard autoclaves must be calibrated every 6
    months
  • You must be trained to use them

13
Disposal of Waste
  • Make sure you follow the University policy
    carefully
  • Ask your supervisor or a senior member of
    technical staff if you do not know the correct
    disposal method for the materials with which you
    are working

14
When in doubt ASK!!!
  • Do not carry out a new or unfamiliar procedure
    until you have been fully trained understand
    the precautions necessary for safe working
  • DO NOT GUESS!!!!
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