Bioproduct Refining

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Bioproduct Refining
Biocatalytic Nanostructure
Biocatalytic Microstructure
Enabling Toolbox
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Bioproduct Refinery
The ability of a biorefinery to achieve market
economics will depend significantly on
improvements in the accessibility of the C-6,
C-5 , and C-3 content of lignocellulose and
hemicellulose and the ability to produce
value-added products concurrently with ethanol,
hydrogen, and biodiesel.
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Bioproduct Modification Pre-Harvest 1.
Alteration of cellulose synthase 2. Replace
cellulose content with starch content 3. Reduce
lignin synthesis 4. Eliminate/reduce xylan side
chain biosynthesis 5. Reduce phenylpropanoid
biosynthesis 6. Eliminate reduce
phenylpropanoid crosslinking
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Bioproduct Modification-Post Harvest 1.
Improved cellulases xylanases 2. Seed-specific
expression of heat tolerant and acid tolerant
depolymerizing enzymes.
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Bioproduct Modification Post-Processing 1.
Catalytic alkylation 2. Selective
denitrification 3. Selective deoxygenation 4.
Selective dehydration 5. Carboxylate
reduction 6. Olefin oxidation 7. Olefin
metathesis 8. Controlled cracking 9.
Controlled reforming
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Biocatalytic Nanostructure
1. Stereoselectivity and regioselectivity vs
basic catalytic activity 2. Dynamic vs. static
structure features single molecule
spectroscopy 3. Enzymology at interfaces 4.
Metalloenzyme assembly 5. Prediction of protein
and enzyme structure 6. Discovery of new
enzymes 7. Improved enyzmes via rational design
and directed evolution
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Biocatalytic Microstructure
1. Transcriptome/proteome analysis 2.
Enzyme-enzyme, enzyme-membrane, enzyme-cell
wall, enzyme-solute matrix 3. Small molecule
import 4. Product export 5. Biocatalytic
platforms 6. Metabolomics
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Enabling Toolbox
1. Promoters inducers host range mRNA
Stability all or none 2. Transcription/Transla
tion codon usage folding proteolysis 3. Gene
shuffling genomic model assisted high
throughput analysis