Stem Cells

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Stem Cells

• Andy Neal
• Gambro BCT Europe

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Questions?

• How many suitable organ donation cadavers are
  available per year?
• 750
• Total number of organs transplanted?
• 2150

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Stem Cells

• What are they?
• When are they used?
• How do you get them?
• What are the issues?

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Stem Cells-What are they?
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Stem Cells-What are they?

• Embryonic
• Harvested from Embryos
• Adult
• Harvested from Patient or Living Donors
• Via Bone Marrow or
• Peripheral Blood

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Stem Cells-What are they?

• Embryonic
• Pluripotent
• Indefinate Undifferentiated growth
• Adult
• Differentiated
• Limited Growth

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Stem Cells-What are they?

• Plasticity?

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Stem CellsWhat are they?
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Stem Cells When are they used?

• Muscle Regeneration
• Tissue Regeneration
• Nerve Regeneration
• Cell Regeneration
• Rescue Dose

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Stem Cells When are they used? -Rescue Dose
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Adult Stem Cells

• How do you get them?
• Bone Marrow Aspiration
• Peripheral Blood Apheresis

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Stem CellsHow do you get them?

• Bone Marrow Aspiration

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Stem CellsHow do you get them?

• Peripheral Blood Apheresis

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Stem CellsWhat are the issues?

• Donor/Patient
• PBSC vs BM
• Equipment types
• Regulatory

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Stem CellsWhat are the issues?Donor/Patient

• Autologous
• Donor availability
• Disease Presence
• Compromised Immune activity
• Allogeneic
• Graft versus Host Disease (GvHD)
• Uncontrolled Proliferation

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Stem CellsWhat are the issues?PBSC vs BM

• PBSC
• Requires Cell Mobilisation
• Donors require Growth Factor
• Cell Number dependent on response

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Stem CellsWhat are the issues?PBSC vs BM

• BM
• Requires Hospital Admission
• Donors require General Anaesthetic
• Just enough cells

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Stem CellsWhat are the issues?Equipment types

• PBSC
• Apheresis Device
• BM
• Apheresis or Centrifugal Device
• Storage
• Manipulation and Cryopreservation

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Stem CellsWhat are the issues?Regulatory

• Medicinal Product
• GMP/GTP
• EU/FDA accreditation

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Stem Cells-Conclusions

• Next Stage of Medicine
• Cellular Immunotherapy
• Foetal Stem cells
• Pure science, clinical application, strong
  emotion and human ethics
• Adult Stem Cells
• Science, Clinical application, Few ethical
  concerns