The%20Effects%20of%20Neo-synephrine%20on%20the%206-Day%20Chicken%20Embryo

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The Effects of Neo-synephrine on the 6-Day
Chicken Embryo

• Sonya Park, Veronica Kvarta,
• and Dr. Jacqueline McLaughlin

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Chicken Heart Development

• The chicken heart develops
• from a series of fusions and
• differentiations.
• Four regions of the heart
• Conotruncus (ct)
• Ventricle (v)
• Atrium (a)
• Sinus venosus (sv)

33 hour chicken embryo heart
72 hour chicken embryo heart
http//www.lv.psu.edu/jxm57/chicklab/outline.html
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Autonomic Nervous System (ANS)

• Involuntary
• Consists of two divisions
• Sympathetic
• Parasympathetic

www.driesen.com/autonomic_nervous_system.htm
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Neo-synephrine

• Typically used as a nasal decongestant
• Some effects of Neo-synephrine
• Arrhythmias
• Low blood pressure
• Tremors
• Restlessness
• Excitability
• Variation of potassium levels
• Neo-synephrine medical warning

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Phenylephrine HCl

• Active ingredient of
• Neo-synephrine
• Alpha adrenergic agonist in sympathetic nervous
  system

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Purpose

• To determine the effects of various
• Neo-synephrine solutions on a 6-day
• in vitro chicken embryo heart.
• Heart Rate (bpm)
• Visible Arrhythmias

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Hypothesis

• The in vitro chicken embryo heart will experience
  visible arrhythmias along with a decrease in
  heart rate (bpm).

6-day in vitro chicken embryo
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Methods

• Stock Solutions
• 0.125, 0.1, 0.075, 0.05, and 0.025 solutions
  of Neo-synephrine were made in chick saline
• Windowed Egg
• obtained control in vivo heart rate
• Extracted Egg
• obtained control in vitro heart rate
• Administered Stock Solutions
• obtained experimental heart rate and noted
  arrhythmias

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RESULTSIn Vivo Heart Rates
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RESULTSIn Vitro Heart Rates
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RESULTSHeart Rates - 0.025
Beats per Minute
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RESULTSHeart Rates - 0.05
Beats per Minute
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RESULTSHeart Rates - 0.075



Beats per Minute



Tachycardia visible Bradycardia visible
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RESULTSHeart Rates 0.10


Beats per Minute




Tachycardia visible Bradycardia visible
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RESULTSHeart Rates 0.125
When the 0.125 solution was administered to each
chicken embryo cardiac arrest occurred. Heart
Rate 0 bpm
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RESULTSAverage Heart Rates
Beats per Minute
In vivo
In vitro
0.025
0.05
0.075
0.10
0.125
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Conclusion

• Due to the fact that noticeable arrhythmias
  occurred in the embryos heart, and there was a
  visible decrease in the heart rate, our results
  support our hypothesis.
• Due to observations, it is obvious that
  Neo-synephrine must be carefully administered
  because high percentage dosages will cause
  dangerous arrhythmias in chicken embryos.
• Further experiments could include
• Test lower concentrations of neo-synephrine to
  see if results are still consistent.
• Administer an antiarrhythmic medication to see if
  arrhythmias occur and heart rate still decreases.

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Siamese Twins!!
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Q A

• Please ask questions during this time.
• Thank you for listening to our presentation!

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Reference

• Neo-synephrine. Medications.com. Skylabs. 4
  Feb. 2004
• lthttp//medications.com/go/drug/Neosynephrinegt.
• Armstrong, Lora L., Goldman, Morton P. et. al.
  Drug Information Handbook 2003-2004. Lexi-Comp,
  Incorporated, March 2003.
• Cruz, Y.P. 1993. Laboratory exercises in
  developmental biology. Academic Press, San Diego,
  California, 241 pages. ISBN 0-12-198390-0
  book