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Barrier Policies and Procedures

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Minimize pathogen exposure to animals held in the UCLA barrier facilities: ... Parasitology. Cell line and tumor testing (IMPACT) Clinical Chemistry. Hematology (CBC) ... – PowerPoint PPT presentation

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Title: Barrier Policies and Procedures


1
Barrier Policies and Procedures
  • Patrick Sharp, DVM
  • UCLA School of Medicine
  • Division of Laboratory Animal Medicine

2
Purpose
  • Minimize pathogen exposure to animals held in the
    UCLA barrier facilities
  • Mac Donald Research Laboratories (MRL)
  • Public Health (B floor)
  • The barrier is a community area
  • The outlined practices are designed to reduce the
    risk of pathogens entering and spreading within
    the facility

3
Scope
  • All investigators housing animals in the barrier
    and those sharing facilities, experimental
    equipment, laboratory space, and/or other such
    circumstances with investigators or individuals
    outside the barrier.

4
Consequences
  • UCLA currently receives over 300 million in
    contract and grant awards, placing us second only
    to Harvard
  • UCLA stands to lose significant resources
    financially and/or staff-wise due to an untoward
    pathogen outbreak
  • Please follow the outlined policies

5
Overview
  • Barrier Use
  • Barrier entry
  • Barrier practices
  • Barrier exit
  • Animal Research Committee (ARC) Concerns
  • Animal Transport in Public Areas
  • Animal Use in Public and Clinical Areas

6
Overview
  • Ordering Animals at UCLA
  • Exporting Animals at UCLA
  • Transferring Animals at UCLA
  • Sentinel Program
  • Division of Laboratory Animal Medicine
  • Technical Services
  • Pharmacy
  • Diagnostic Laboratory

7
Barrier Use
  • Barrier entry
  • Barrier practices
  • Barrier exit

8
Barrier Entry
  • Impacts four areas
  • Scientific personnel
  • Physical plant personnel
  • Animals
  • Equipment

9
Barrier Entry
  • Gowns
  • Booties
  • Cap
  • Mask
  • Gloves
  • Equipment

10
Barrier Entry
  • Gowns
  • Put on a new gown every time you enter the
    facility
  • Throw the gown away if it touches the floor
  • Additional gowns may be need to be worn in some
    rooms

11
Barrier Entry
  • Booties
  • CHS Foot bath
  • Step into the foot bath while donning the booties
  • MRL
  • Spray an area outside the red area with
    disinfectant
  • Step into this area donning the booties
  • Wipe your feet in the sprayed area
  • Additional booties may be need to be worn in some
    rooms

12
Gloves
  • Put on gloves before handling animals
  • Put on gloves before wiping down items which may
    be brought into the barrier
  • Glove etiquette

13
Equipment
  • Materials brought into the barrier must be
    contained within a plastic bag.
  • Generously spray down the plastic bag contained
    items with the disinfectant
  • Wipe the outside of the bag with your gloved
    hands
  • Open the closed plastic bag ONLY in the cage
    change station.

14
Equipment
  • Non-barrier hand carts do not enter the barrier
  • Barrier hand carts do not leave the barrier
  • Disinfect before each use
  • Disinfect after each use

15
Animals
  • Importing or exporting animals
  • Dierk Biggs 51826 ltdbiggs_at_mednet.ucla.edugt
  • Animal transfers
  • Ken Jones 57140 ltkjones_at_mednet.ucla.edugt
  • Cell lines and tumors passed in mice must be
    tested for murine pathogens
  • Jerry Werk 56497 ltjwerk_at_mednet.ucla.edugt

16
Special Housing/Husbandry
  • Biohazard
  • Radioactive materials

17
Other
  • Human cell lines
  • Human tumors

18
Barrier Practices
  • Keep your eyes open
  • Ask questions
  • Traffic
  • Bag-up items BEFORE entering the facility
  • Bag-up items BEFORE leaving an animal room
  • Work from cleanest to dirtiest
  • Minimize traffic within the facility

19
Barrier Practices
  • Cage change station etiquette
  • Turn on
  • Wipe down the hood bottom AND sides
  • Handle animals
  • with tongs if possible, disinfecting between
    cages AND when finished
  • gloved hands, disinfect between cages
  • Bag up supplies/animals before leaving the room
  • Wipe down the hood when finished bottom AND sides

20
Barrier Practices
  • Animals
  • Open cages within a cage change station or hood
  • Disinfect gloved hands between cages
  • Do not place animals on microisolator tops Wire
    tops are OK

21
Barrier Practices
  • Animal Escapes
  • Euthanize the animals
  • Identify cages as Change Last so these cages
    can be changed prior to the sentinels
  • Humane Animal Traps

22
Barrier Practices
  • When exiting your animal room
  • Follow the posted exit procedures
  • All clothing put on immediately prior to entering
    the animal room
  • Must be removed before exiting the room, and
  • Disposed of in the room you are exiting

23
Barrier Practices
  • Room lighting
  • Euthanizing animals
  • Clean cages
  • Pets Rodents, reptiles, etc.

24
Exiting the Barrier
  • Impacts four areas
  • Scientific personnel
  • Physical plant personnel
  • Animals
  • Equipment

25
Exiting the Barrier
  • Personnel
  • Do not wear barrier clothes outside the barrier
  • This includes gloves and booties
  • Plan on not returning to the barrier

26
Exiting the Barrier
  • Animals exiting the barrier must be appropriately
    handled
  • Covered by an opaque material (black garbage bag)
  • Cardboard transport box

27
Exiting the Barrier
  • Animals cannot be housed outside of the barrier
    facility over 12 hours without Animal Research
    Committee (ARC) approval
  • Contact the ARC for approval
  • 310.20.66308
  • ltwww.oprs.ucla.edu/animalgt

28
Exiting the Barrier
  • Animals leaving the barrier MUST NOT return to
    the barrier
  • They MUST be placed in the Animal Return Room
  • Matthew Dang 310.82.54080 ltmdang_at_mednet.ucla.edu
    gt
  • Ken Jones 310.82.57140
  • ltkjones_at_mednet.ucla.edugt

29
Animal Research Committee (ARC)
  • Location 2107 Ueberroth Building
  • Phone 310.20.66308
  • Fax 310.79.49565
  • Website www.oprs.ucla.edu/animal
  • Animal Transport Through Clinical and Public
    Areas
  • Animal Use in Clinical and Public Areas

30
Animal Transport Through Clinical and Public Areas
  • Rodents must be transported in covered shoebox
    cages or transport boxes
  • Double bag dead animals in an opaque durable
    plastic bag of appropriate size.

31
Animal Transport Through Clinical and Public Areas
  • Transporting animals in vehicles not specifically
    designated for animal transportation (e.g.
    personal or rented vehicles) is strongly
    discouraged
  • Please contact the Division of Laboratory Animal
    Medicine (x57281) for details concerning animal
    transport in vehicles

32
Animal Transport Through Clinical and Public Areas
  • Service elevators must be used when available
  • This includes the transportation of empty
    carriers, restraint devices, transport cages,
    shoebox cages, etc.

33
Animal Use in Clinical and Public Areas
  • Please follow the Animal Transport Through
    Clinical and Public Areas guidelines
  • Patients and non-essential employees must not be
    present
  • Employees participating in the procedure should
    be listed on the Animal Research Committee
    approved protocol

34
Animal Use in Clinical and Public Areas
  • Door and interior window shades in the procedure
    room should be closed to avoid view and/or
    intrusion
  • Countertops, floors, equipment, and other areas
    of the procedure room where animals were used
    should be thoroughly cleaned and disinfected
    after procedures have been performed

35
Animal Use in Clinical and Public Areas
  • Disposal of animal waste, trash, and debris shall
    comply with applicable federal, state, and local
    laws relating to protection of the environment
    and pollution control

36
Ordering Animals at UCLA
  • Animal sources
  • Order Online At www.dlam.ucla.edu/
  • Animals
  • Online Animal Order Form
  • Importing
  • Domestic
  • International
  • Transferring
  • Exporting
  • Domestic
  • International

37
Ordering Animals at UCLA
  • There are essentially three sources of research
    rodents at UCLA
  • Approved Sources,
  • Unapproved Sources, and
  • Internal Transfer

38
Ordering Animals at UCLA
  • Approved Sources include
  • Vendors
  • Harlan
  • Taconic, and others
  • Other organizations providing us regular sentinel
    reports
  • Contact Tensie Palmer
  • Orders MUST be received prior to 4 PM Tuesday
  • Phone 310.82.57281
  • E.mail lttpalmer_at_mednet.ucla.edugt
  • Animals are available the Monday after their
    arrival

39
Ordering Animals at UCLA
  • Unapproved Sources include any organization not
    providing UCLA regular sentinel information
  • Universities
  • Government
  • Others
  • Contact Jamie Tran
  • Phone 310.79.47931
  • E.mail ltjtran_at_mednet.ucla.edugt

40
Ordering Animals at UCLA
  • Ordering from Unapproved Sources
  • Give at least three weeks notice
  • Complete an Animal Shipping Record
  • ltwww.dlam.ucla.edugt
  • Click on Forms and Records
  • Select Animal Shipping Record
  • Online submission
  • Download the document

41
Ordering Animals at UCLA
  • Ordering from Unapproved Sources
  • Once a shipment has been approved it has one of
    three destinations
  • Special Quarantine
  • Deferred Quarantine
  • Biocontainment

42
Ordering Animals at UCLA
  • Special Quarantine
  • At least 6 weeks
  • Individual testing
  • Sentinel testing

43
Ordering Animals at UCLA
  • Deferred Quarantine
  • Animals must appear to be clean based on the
    sentinel information provided by the source
    institution
  • All animals used Monday 8 AM following their
    arrival at UCLA
  • Testing of some/all sacrificed animals

44
Ordering Animals at UCLA
  • Biocontainment
  • Short term
  • Source of infection of clean animals housed in
    barrier and defined flora facilities
  • Requires rederivation to enter the clean
    facilities
  • Cesarean
  • Embryo Transfer

45
International Imports
  • Must meet these agency requirements
  • US Treasury
  • US Customs
  • US Department of Agriculture (USDA)
  • Animal and Plant Health Inspection Service
    (APHIS)
  • US Department of Health and Human Services
  • Centers for Disease Control and Prevention (CDC)

46
Transferring Animals at UCLA
  • Internal Transfer
  • Complete an Animal Transfer Record
  • ltwww.dlam.ucla.edugt
  • Click on Forms and Records
  • Select Animal Transfer Record
  • Ken Jones
  • Phone 310.82.57140
  • CHS 1V-203
  • Document animal traffic between rooms,
    facilities, investigators, and protocol numbers
  • Six week quarantine
  • Adds validity to the Sentinel Program

47
Exporting Animals From UCLA
  • Complete an Animal Shipping Record
  • ltwww.dlam.ucla.edugt
  • Click on Forms and Records
  • Select Animal Shipping Record
  • Online submission
  • Download the document
  • The Division will provide the receiving
    institution with the previous 9 months of
    sentinel information
  • Please DO NOT provide the sentinel information to
    reduce confusion

48
Exporting Animals From UCLA
  • The receiving institution may do one of the
    following
  • Accept the shipment
  • Immediate export
  • Delayed (space availability)
  • Reject the shipment
  • Health
  • Space availability

49
Exporting Animals From UCLA
  • Upon accepting the shipment the receiving
    institution will provide the Division with a
    shipping address
  • The animals will need to be inspected with in 10
    days of departure, and a USDA Health Certificate
    may need to be issued

50
Exporting Animals From UCLA
  • The UCLA investigator is currently responsible
    for making the shipping arrangements
  • Animals are to be shipped on a Monday, Tuesday,
    or Wednesday of a normal week

51
Exporting Animals From UCLA
  • Three pieces of information must be collected
    from the transporting agency
  • Courier
  • Tracking number
  • Expected date of arrival
  • The Division will provide this shipping
    information to the Receiving Institution

52
Exporting Animals From UCLA
  • Ship ONLY the number of animals indicated on the
    Animal Shipping Record

53
International Exports
  • Must meet these requirements
  • USDA
  • Health Inspection Valid for 10 days
  • Endorsed Health Certificate Valid for 30 days
  • Country of Destination
  • International Air Transport Association

54
Sentinel Program
  • High-risk animals
  • Monitoring
  • Gross lesions
  • Microscopic lesions
  • Serology ELISA, IFA, HAI
  • Molecular

55
The UCLA Rodent Sentinel Program
  • Animals housed on a sample of dirty bedding from
    all other rodents on a given rack
  • Research animals must not be moved from one rack
    to another
  • Marked with a Sentinel sticker on the cage card
  • Do not use or euthanize these animals

56
The UCLA Rodent Sentinel Program
  • The animals are necropsied and blood is collected
    for serology
  • If gross lesions are observed then histopathology
    is performed
  • All serologies from a given sentinel quarter are
    sent to the same diagnostic laboratory for
    consistency

57
The UCLA Rodent Sentinel Program
  • Ectromelia
  • Epizootic Diarrhea of Infant Mice (EDIM)
  • Lymphocytic Choriomeningitis (LCM)
  • Minute Virus of Mice (MVM)
  • Mouse Hepatitis Virus (MHV)
  • Mouse Parvovirus
  • Mycoplasma pulmonis
  • Pneumonia Virus of Mice (PVM)
  • Reovirus
  • Sendai
  • Theilers Murine Encephalomyelitis (TMEM)
  • Endoparasites Pinworms
  • Ectoparasites Mites

58
The UCLA Rodent Sentinel Program
  • Additional serology panels are available
  • Cost/Sample requirements
  • Contact Jerry Werk
  • Phone 310.82.56497
  • E.mail ltjwerk_at_mednet.ucla.edugt

59
Division of Laboratory Animal Medicine Services
  • Training
  • Technical services
  • Pharmaceutical and research supplies
  • Division website www.dlam.ucla.edu
  • Pathology and research support services

60
Division of Laboratory Animal Medicine
  • Training FREE
  • Technical service
  • Fee for service basis
  • 6.00/15 minutes (AHT)

61
Division of Laboratory Animal Medicine
  • Pharmaceuticals, equipment, and supplies for
    animal research may be purchased through the
    Divisions Clinic
  • Controlled substances can be purchased from the
    hospital pharmacy
  • Contact Talia Acosta
  • Phone 310.79.44819
  • E.mail lttacosta_at_mednet.ucla.edugt

62
Division of Laboratory Animal Medicine
  • Website
  • ltwww.dlam.ucla.edugt
  • Let us know if something should be added

63
Division of Laboratory Animal Medicine
  • Necropsy
  • Serology
  • Bacteriology
  • Parasitology
  • Cell line and tumor testing (IMPACT)
  • Clinical Chemistry
  • Hematology (CBC)
  • Urinalysis
  • Histopathology
  • Characterizing KO/Tg animals

64
Division of Laboratory Animal Medicine Veterinary
Faculty
  • Timothy Lawson, DVM
  • 310.82.53178 lttlawson_at_mednet.ucla.edugt
  • Director, Division of Laboratory Animal Medicine
  • Diplomate, American College of Laboratory Animal
    Medicine

65
Division of Laboratory Animal Medicine Veterinary
Faculty
  • Patrick Sharp, DVM
  • 310.82.52776 ltpsharp_at_mednet.ucla.edugt
  • Associate Director, Division of Laboratory Animal
    Medicine
  • Diplomate, American College of Laboratory Animal
    Medicine

66
Division of Laboratory Animal Medicine Veterinary
Faculty
  • Rebecca Schwiebert, DVM, PhD
  • Phone 310.82.56240
  • E.mail rschwiebert_at_mednet.ucla.edu
  • Director, Primate, Minor Species, and Clinical
    Training Services
  • Diplomate, American College of Laboratory Animal
    Medicine

67
Division of Laboratory Animal Medicine Veterinary
Faculty
  • JanLee Jensen, DVM
  • Phone 310.20.66786
  • E.mail jjensen_at_mednet.ucla.edu
  • Director, Surgical Services
  • Diplomate, American College of Veterinary
    Internal Medicine

68
Division of Laboratory Animal Medicine Veterinary
Staff
  • Gregory Lawson, DVM, PhD
  • Phone 310.82.58514
  • E.mail glawson_at_mednet.ucla.edu
  • Director, Diagnostic and Rodent Services
  • Eligible, American College of Veterinary Pathology
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