EVC

1
Sort ReportAmara Lab / Pragati NigamCD3 CD4
CD25 subsets from non-infected rhesus macaque

• EVC / Liza Bronner / lbronne_at_emory.edu / Primary
  Operator
• EVC / Suzanne Mertens / saguila_at_emory.edu /
  Supervisor
• DiVa Experiment filename 080116 RAA-Pragati
  naive

2
The Results in Numbers
Target population CD4- CD25- CD4- CD25 CD4 CD25 CD4 CD25-
Starting frequencies 20.7 0.9 5.2 16.9
Starting cell count 78 x 10e6 78 x 10e6 78 x 10e6 78 x 10e6
Final Purity 100 96 98 100
Total sorted events 5,101,638 211,019 1,222,885 3,812,751
Total threshold events 41,265,473 41,265,473 41,265,473 41,265,473
Sort gating This will exclude dead cells and
any non-singlet cells. Total sort count
Accumulated number of events passing the last
sensor before the collection tube. This number is
the highest possible number of cells in the sort
collection tube. Discrepancies higher than 20
between this number and any subsequent cell count
by researcher may indicate loss due to improper
sort stream alignment, subsequent spin-down of
cells out of sort buffer, subsequent post-sort
preparations, or cell shattering upon impact with
sort collection tube.
Billable time 4.0 hours (exact times now
automatically collected by Diva login)
3
Pre SortCD3 uninfected rhesus macaque
PBMCfor CD4CD25CD4-CD25CD4 CD25-CD4-
CD25-
4
Post Sort
CD4- CD25-
5
Post Sort
CD4- CD25
6
Post Sort
CD4 CD25
7
Post Sort
CD4 CD25-
8
QC for all detectors
Manual QC has been replaced with CST. Reports
will be included for next sort, named as
Cytometer Performance Report in clients
FileServer folder for the specific sort in
question. This is the next generation in Aria
sorter manufacturers automation of quality
control testing. CST was made possible by EVC
Flow Cytometry Cores decision to participate in
beta testing of the manufacturers upcoming
software upgrade for the Aria.