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Lab 1' Microscopy

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Lab 1' Microscopy – PowerPoint PPT presentation

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Title: Lab 1' Microscopy


1
Lab 1. Microscopy
2
Microscope
  • Microbiology study organisms too small to be seen
    with the naked eye
  • Father of microscopy Anton van Leeuwenhoek
    (1632-1723)
  • First to describe bacteria
  • Any two dots that are closer together than 0.275
    micrometers will be seen as a single dot
    (resolution)
  • Any object smaller than 0.275um will be invisible
    (magnification)
  • 1000um 1mm

3
Microscope
  • Base
  • Stage (where you put the slide)
  • Arm
  • Light source, condenser
  • Diaphragm ? modify light going through contrast
  • Objectives lenses 4X, 10X, 40X, 100X
  • Ocular or eyepieces 10X
  • Objective X ocular total magnification

4
Microscope anatomy
http//shs.westport.k12.ct.us/mjvl/biology/microsc
ope/microscope.htm
5
How to use
  • Carry with both hands one hold the arm the other
    the base
  • Plug it in, turn on
  • Use the focus knob to take the stage away from
    the objective when you put the slide on
  • Secure the slide with the clamps
  • Look first using 4X objective
  • Focus using the large knob (coarse) and then the
    small knob (fine)

6
How to focus
  • Coarse adjustment at 4X using the coarse knob,
    fine knob for final touch
  • Once everything is in focus you can switch to
    higher objective
  • If lose focus go back to previous magnification
  • Troubleshooting cant focus
  • Lens dirty ? use lens cleaner paper
  • practice

7
.
  • Again always focus with low power first
  • Go from 4X to 10X to 40X to 100X
  • You need oil for 100X objectives (we will do this
    next week)
  • Turn the light off if you are not using the
    microscope

8
What not to do
  • Hold the microscope by the arm only
  • Try to focus at high power first
  • Use oil at 100X and then go back at 40X (oil get
    sucked up in the 40X lens and very hard to clean)
  • Put the slide on without lowering the stage

9
Wet mount
  • Suspend the yeast preparation swirl it gently
  • Transfer a small drop on the slide using the
    pipette
  • Place a cover slip on top
  • Put on the stage (coverslip up, slide underneath)
    ? observe
  • manipulate slide and cover slip by their
    sides

10
Wet mount
www.sporeworks.com/ labnotes/microscopytek.html
11
Yeast

http//www.lima.ohio-state.edu/academics/biology/i
mages/yeast.jpg
  • http//fig.cox.miami.edu/cmallery/255/255hist/yea
    st.jpg

12
Hanging drop slide
  • Place a ring of Vaseline around the edge of a
    coverslip
  • Using the pipette place a drop of the Artemia in
    the center of the coverslip
  • Place the depression slide on the coverslip
  • Turn the slide over

13
Hanging drop slide
Source Case, 2004
14
Remember the Objectives of the laboratory 1
  • Learn how to focus
  • Look at 1 histology slide ? draw your observation
    at 2 magnification
  • Make wet mount of yeast ? draw your observation
  • Hanging drop slide of water with artemia cyst ?
    draw your observation
  • Everybody has one microscope ? find a lab partner
    for next time
  • Record lab results in the notebook
  • Dont forget to sign the sheet about lab rules
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