This week (AM):

1

• This week (AM)
• Wednesday
• Repeat PCR of environmental isolates
• Make gels for next time
• Thursday
• Run gel of PCR
• PCR cleanup
• Sequencing reaction (see handout)
• Intro to BLAST

2

• This week (PM)Wednesday -Run gel of the last
  PCR (if you have one) -Sequence DNA (see
  sequencing handout)Thursday -sequencing
  cleanup -intro to BLAST