RAS

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RAS
?
RAL-GEF
PI3K
RAF
proliferation
U0126 PD98059 PD184352 (CI-1040)
MEK
AKT
ERK
proliferation survival
proliferation / differentiation survival migration
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The Cell Cycle
Mitosis M phase
Equal division of chromosomal content into two
daughter cells
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Growth Curves U0126 causes a reduc- tion in
cell number. Cells accumulate in G1.
days
days
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Enzyme catalysis What are enzymes really doing?
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Enzyme kinetics (Michaelis Menten)
Km Michaelis constant k1, k-1, k2 rate
constants for the individual steps
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1. Enzyme catalysis
2. Competitive inhibition
3. Noncompetitive inhibition
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Enzyme inhibition competitive vs noncompetitive