Do you have a gene target to TILL Heres what you need to know - PowerPoint PPT Presentation

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Do you have a gene target to TILL Heres what you need to know

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4 attempts to find primers for TILLING (most targets pass after 2 primer sets) ... 180 day confidentiality period. Sequence TILLed will be deposited into GenBank ... – PowerPoint PPT presentation

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Title: Do you have a gene target to TILL Heres what you need to know


1
Do you have a gene target to TILL? Heres what
you need to know
  • Fees policies
  • 750 fee per gene target (NSF subsidizes the
    rest).
  • 4 attempts to find primers for TILLING (most
    targets pass after 2 primer sets)
  • Allelic series at least one of the mutations
    has 95 probability of being damaging
  • If order is not completed with current
    population, we will keep your order active and
    screen additional lines as they become
    available.
  • Select Region to TILL using CODDLe (see laminated
    sheets and our website for a tutorial).
  • Primer Pre-screening (Updated protocols now on
    our website)
  • MTP will pre-screen all primers
  • Expedite your order by testing the primers in
    your lab first
  • Advantage you can test several primer sets at
    once
  • Mutation Delivered
  • E-mail with link to PARSESNP report that
    describes the potential effect of the mutation(s)
    as well as any restriction sites gained and lost
  • Link to a web-based form to order seed stock
  • 180 day confidentiality period
  • Sequence TILLed will be deposited into GenBank
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