Advance made here: cationic nanoparticles stabilize phospholipid vesicles up to dense volume fractions without fusion, allowing ligand-receptor binding.

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NIRT Actively Reconfigurable Nanostructured
Surfaces for the Improved Separation of
Biological Macromolecules Ravi Kane, Steve
Granick, and Sanat Kumar, CBET - 0608978
Particle binding induces charge separation and
slaved motion
Nanoparticle-stiffened phospholipid vesicles
Fluorescence autocorrelation functions showing
that streptavidin binds effectively to
vesicle-attached biotin even when nanoparticles
stabilize the liposomes to which biotin is
attached.
t0 min
t20 min
?p200 mM
?p100 mM
?p300 mM
?p400 mM
Epifluorescence images of naked GUVs (top) and
nanoparticle-stabilized GUVs (bottom) reveal
enhanced osmotic stress tolerance for the latter.
Enzyme Adsorption onto Raft-Like Domains
Increases Stability
Advance made here cationic nanoparticles
stabilize phospholipid vesicles up to dense
volume fractions without fusion, allowing
ligand-receptor binding. In progress
interactions with DNA, especially plasmid DNA.
DiI
FITC-SBP
Merged
Adsorption of SBP onto small domains (5 nm)
imparts greater stability than larger domains
(13 nm)
Heterogeneous (rapid quench) Heterogeneous (slow
quench) Homogeneous
Soft Matter 3, 551 (2007) J. Phys. Chem. C
111, 8233 (2007)
JACS 131, 7107 (2009)