Ling V. Sun

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PBmice and MP-PBmice A database system of
PiggyBac (PB) insertional mutations in mice and a
subsystem for mapping the mutations onto the
mouse genome with large-scale experimental data
Ling V. Sun Li Wang
Institute of Developmental Biology and Molecular
Medicine , Fudan University, and Shanghai Center
for Bioinformation Technology, China
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Transposons and Insertional Mutations
Transposons Mobile Genetic Elements
Transgenesis
Barbara McClintock
chromosome
Gene
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Advantages of Insertional Mutations

• can produce easily tractable mutations
• can produce large number of mutants at low cost
  and high speed

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PB(PiggyBac) a high efficient transposition
system in mammals
Moth piggyBac (Cary et al. Virology 1989)
Mammal PB system (Ding et al. Cell 2005)
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PB an ideal tool for insertional mutagenesis
Traditional Knock-out
PB insertional mutagenesis
Molecular cloning
ES
Reduce Cost Improve Efficiency
Screen cell lines
Micro-injection
IVF
Breeding
PB
Mutant
Mutant 1
Mutant 2
Mutant 3
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http//www.idmshanghai.cn/PBmice
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INSERT interface
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MP-PBmice system (insertional mutations mapping
system of PBmice

• Experimental Procedure
• Flow production

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From PBmice to MP-PBmice

• Integrated information of PB insertional
  mutations and their characterizations in mice
  PBmice (NAR, 2008 vol.36, Http//www.idmshanghai.c
  n/PBmice)
• Daily book-keeping and information exchange
  between experimental groups for the mapping
  procedure MP-PBmice

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• Design and implementation
• User Interfaces
• Features

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MP-PBmice

• Design and implementation
• User Interface
• Features

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Database Tables the relational data schema

• Access-control Table
• Experimental Stage Table
• Constant Table
• Status Table

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Model-View-Controller (MVC) Pattern
Struts-Spring-Hibernate (SSH) Framework
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MP-PBmice

• Design and implementation
• User Interfaces
• Features

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viewDetail
detailed information page
comment box
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MP-PBmice

• Design and implementation
• User Interface
• Features

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Strict Access Control
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Work Flow Control
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Expandability

• Suitable for any genome
• Suitable for any experimental mapping method

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Future Plan

• Improve PBmice Mapping Database
• Add Bioinformatics Tools Needed

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Acknowledgments
Wenwei Yang Min Han Yuan Zhuang Tian Xu Xiaohui
Wu Yun Zheng
Ke Jin Jigang Yang Yang Zhong
Xing Xie Dongsheng Li Ning Gu
For collaboration and further contact Dr. Ling
V. Sun (PI) Lingsun_at_fudan.edu.cn
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Thanks