OsO4 stain on polar surfaces shows up black with electron illumination

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OsO4 stain on polar surfaces shows up black with
electron illumination
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STEROLS in membranes
sitosterol
Ergosterol
stigmasterol
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Note absence of glycolipids (found in chloroplast
membranes)
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Fluid mosaic model
free lateral movement
restricted lateral movement
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Amphipathic Liquid Aggregates
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Erythrocyte membrane
Determined by treatment with phospholipase C
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Integral and peripheral proteins
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Tetrasaccharide Neu5Ac- Neu5Ac-Gal-GalNAc
Other oligosaccharide
Glycophorin- Integral membrane protein of
erythrocytes
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Hydropathy Indices G -0.4 A 1.8 P 1.6 V
4.2 L 3.8 I 4.5 M 1.9 F 2.8 Y -1.3 W
-0.9
S -0.8 T -0.7 C 2.5 N -3.5 Q -3.5 K
-3.9 H -3.2 R -4.5 D -3.5 E -3.5
Hydrophobicity of sequence estimated by rolling
average of hydropathy index of 7 residues
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W (Red) and Y (Orange) residues found at interface
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From E. Coli outer membrane
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